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Protein

Proteasome subunit beta type-2

Gene

pbs-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_101459. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_183279. ER-Phagosome pathway.
REACT_183284. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_183296. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_184635. Orc1 removal from chromatin.
REACT_205379. SCF-beta-TrCP mediated degradation of Emi1.
REACT_215136. Asymmetric localization of PCP proteins.
REACT_243979. CDK-mediated phosphorylation and removal of Cdc6.
REACT_249811. Degradation of beta-catenin by the destruction complex.
REACT_254516. SCF(Skp2)-mediated degradation of p27/p21.
REACT_255237. degradation of DVL.
REACT_261912. Ubiquitin-dependent degradation of Cyclin D1.
REACT_263250. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_263687. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Protein family/group databases

MEROPSiT01.984.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-2 (EC:3.4.25.1)
Alternative name(s):
Proteasome subunit beta 4
Gene namesi
Name:pbs-4
ORF Names:T20F5.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiT20F5.2; CE13818; WBGene00003950; pbs-4.

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. proteasome core complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Proteasome subunit beta type-2PRO_0000148046Add
BLAST

Proteomic databases

PaxDbiP91477.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).By similarity

Protein-protein interaction databases

STRINGi6239.T20F5.2.

Structurei

3D structure databases

ProteinModelPortaliP91477.
SMRiP91477. Positions 1-196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000188743.
InParanoidiP91477.
KOiK02734.
PhylomeDBiP91477.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P91477-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHFLVGISTE NYVILAADKA TFAYGAILAD SENDKEYRLG KKLTMMCIGE
60 70 80 90 100
EGDVAQFGDW TKRNLQLYSV RNGYEVSPSC AHHFVRRSIA EGLRSQDHYT
110 120 130 140 150
VDVLIGGYDD KEDKAFLGSV DYLANGLGQQ PYLFRGFCGR FCYAIMDREY
160 170 180 190
KKDMTEAEGL ALMNKCIGEA KRRFVANIPG YKVVIIDKKG YRKLDDVLF
Length:199
Mass (Da):22,549
Last modified:October 18, 2001 - v2
Checksum:i27510AF7CBA8DEBF
GO

Sequence cautioni

The sequence CCD67779.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080916 Genomic DNA. Translation: CCD67779.1. Different initiation.
PIRiT29206.
RefSeqiNP_491261.1. NM_058860.6.
UniGeneiCel.20577.

Genome annotation databases

GeneIDi171975.
KEGGicel:CELE_T20F5.2.
UCSCiT20F5.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080916 Genomic DNA. Translation: CCD67779.1. Different initiation.
PIRiT29206.
RefSeqiNP_491261.1. NM_058860.6.
UniGeneiCel.20577.

3D structure databases

ProteinModelPortaliP91477.
SMRiP91477. Positions 1-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.T20F5.2.

Protein family/group databases

MEROPSiT01.984.

Proteomic databases

PaxDbiP91477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171975.
KEGGicel:CELE_T20F5.2.
UCSCiT20F5.2. c. elegans.

Organism-specific databases

CTDi171975.
WormBaseiT20F5.2; CE13818; WBGene00003950; pbs-4.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000188743.
InParanoidiP91477.
KOiK02734.
PhylomeDBiP91477.

Enzyme and pathway databases

ReactomeiREACT_101459. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_183279. ER-Phagosome pathway.
REACT_183284. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_183296. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_184635. Orc1 removal from chromatin.
REACT_205379. SCF-beta-TrCP mediated degradation of Emi1.
REACT_215136. Asymmetric localization of PCP proteins.
REACT_243979. CDK-mediated phosphorylation and removal of Cdc6.
REACT_249811. Degradation of beta-catenin by the destruction complex.
REACT_254516. SCF(Skp2)-mediated degradation of p27/p21.
REACT_255237. degradation of DVL.
REACT_261912. Ubiquitin-dependent degradation of Cyclin D1.
REACT_263250. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_263687. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Miscellaneous databases

NextBioi873489.
PROiP91477.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiPSB2_CAEEL
AccessioniPrimary (citable) accession number: P91477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 18, 2001
Last modified: January 7, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.