P91427 (PGK_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk-1
ORF Names:	T03F1.3

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB phosphoglycerate kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Probable phosphoglycerate kinase		PRO_0000145844
Regions
	Nucleotide binding	372 – 375	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	63 – 66	4	Substrate binding By similarity
Sites
	Binding site	39	1	Substrate By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	170	1	Substrate By similarity
	Binding site	219	1	ATP By similarity
	Binding site	312	1	ATP; via carbonyl oxygen By similarity
	Binding site	343	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P91427 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: CB0369287AFE5AC4	FASTA	417	44,113
	10 20 30 40 50 60 MSSLNKLAID QLNLAGKRVL IRVDFNVPLK DGKITNNQRI AAAVPTIQHA LSNGAKSVVL 70 80 90 100 110 120 MSHLGRPDGR RQDKYTLKPV AEELKALLKK DVLFLDDCVG SEVEAACADP APGSVILLEN 130 140 150 160 170 180 LRYHLEEEGK GVDASGAKVK ADSAAVKKFR ESLTKLGDIY VNDAFGTAHR AHSSMVGVEH 190 200 210 220 230 240 SQRASGFLLK NELSYFSKAL DNPARPFLAI LGGAKVADKI QLIKNLLDKV NEMIIGGGMA 250 260 270 280 290 300 YTFLKVAQGV KIGNSLYDEE GAKIVNELLE AAKAKGVQIH LPVDFVIADK FAEDATSKTV 310 320 330 340 350 360 TAEEGVPDGH MGLDVGPESS KIFAAAIQRA KTIVWNGPAG VFEFDKFATG TKSLMDEVVK 370 380 390 400 410 ATAAGAITII GGGDTATAAK KYNTEDKVSH VSTGGGASLE LLEGKVLPGV DALSPAQ

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080917 Genomic DNA. Translation: CCD67788.1.
PIR	T29198.
RefSeq	NP_491245.1. NM_058844.4.
3D structure databases
ProteinModelPortal	P91427.
SMR	P91427. Positions 5-414.
ModBase	Search...
Protein-protein interaction databases
IntAct	P91427. 1 interaction.
MINT	MINT-226425.
STRING	6239.T03F1.3.
2D gel databases
World-2DPAGE	0020:P91427.
Proteomic databases
PaxDb	P91427.
PRIDE	P91427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T03F1.3.1; T03F1.3.1; T03F1.3. T03F1.3.2; T03F1.3.2; T03F1.3.
GeneID	171965.
KEGG	cel:CELE_T03F1.3.
UCSC	T03F1.3. c. elegans.
Organism-specific databases
CTD	171965.
WormBase	T03F1.3; CE13100; WBGene00020185; pgk-1.
Phylogenomic databases
eggNOG	COG0126.
GeneTree	ENSGT00390000008820.
HOGENOM	HOG000227107.
InParanoid	P91427.
KO	K00927.
OMA	NSCIGDE.
Enzyme and pathway databases
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	873451.

Entry information

Entry name

PGK_CAEEL

Accession

Primary (citable) accession number: P91427

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project