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Protein

Serine/threonine-protein phosphatase PP1-delta

Gene

gsp-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probable phosphatase which plays a redundant role with gsp-4 in spermatogenesis by regulating sister chromatid segregation during meiosis (PubMed:16943775, PubMed:22042574). In addition, involved in sperm motility by controlling the dynamic disassembly of major sperm proteins (MSP) in the spermatozoan pseudopodium (PubMed:22042574).2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Manganese 1By similarity
Metal bindingi64 – 641Manganese 1By similarity
Metal bindingi90 – 901Manganese 1By similarity
Metal bindingi90 – 901Manganese 2By similarity
Metal bindingi122 – 1221Manganese 2By similarity
Active sitei123 – 1231Proton donorBy similarity
Metal bindingi172 – 1721Manganese 2By similarity
Metal bindingi247 – 2471Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • male meiosis chromosome segregation Source: WormBase
  • oviposition Source: WormBase
  • regulation of pseudopodium assembly Source: WormBase
  • reproduction Source: WormBase
  • spermatogenesis Source: UniProtKB-KW
  • sperm motility Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-deltaCurated (EC:3.1.3.16UniRule annotation)
Alternative name(s):
CeGLC-7-deltaCurated
Glc seven-like phosphatase 4Imported
Gene namesi
Name:gsp-4Imported
ORF Names:T03F1.5Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiT03F1.5; CE13104; WBGene00020187; gsp-4.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: WormBase
  • cytoplasm Source: WormBase
  • nuclear chromatin Source: WormBase
  • pseudopodium Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Chromosome, Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype (PubMed:22042574). Simultaneous RNAi-mediated knockdown of gsp-3 and gsp-4 results in male sterility due to chromosome segregation defects during sperm meiosis (PubMed:16943775). gsp-3 and gsp-4 double mutant hermaphrodites have egg laying defects and no viable progeny. gsp-3, gsp-4 and him-8 triple mutants have incomplete separation of sister chromatids during the second meiotic segregation. Mislocalization of MSP in activated sperm. In addition, have partial reduction in mpk-1 phosphorylation and air-2 association with bivalent chromosomes in the most proximal oocyte in response to MSP (PubMed:22042574).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Serine/threonine-protein phosphatase PP1-deltaCuratedPRO_0000436389Add
BLAST

Proteomic databases

EPDiP91420.
PaxDbiP91420.

Expressioni

Tissue specificityi

Expressed in male germline including spermatocytes, spermatids and spermatozoa.2 Publications

Developmental stagei

Expressed at all stages of spermatocyte meiosis.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi6239.T03F1.5.1.

Structurei

3D structure databases

ProteinModelPortaliP91420.
SMRiP91420. Positions 5-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
InParanoidiP91420.
KOiK01090.
OMAiHETPAIN.
PhylomeDBiP91420.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P91420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATIDVDNL MSRLLNVGMS GGRLTTSVNE QELQTCCAVA KSVFASQASL
60 70 80 90 100
LEVEPPIIVC GDIHGQYSDL LRIFDKNGFP PDINFLFLGD YVDRGRQNIE
110 120 130 140 150
TICLMFCFKI KYPENFFMLR GNHECPAINR VYGFYEECNR RYKSTRLWSI
160 170 180 190 200
FQDTFNWMPL CGLIGSRILC MHGGLSPHLQ TLDQLRQLPR PQDPPNPSIG
210 220 230 240 250
IDLLWADPDQ WVKGWQANTR GVSYVFGQDV VADVCSRLDI DLVARAHQVV
260 270 280 290 300
QDGYEFFASK KMVTIFSAPH YCGQFDNSAA TMKVDENMVC TFVMYKPTPK

SLRKG
Length:305
Mass (Da):34,584
Last modified:May 1, 1997 - v1
Checksum:iF0EB55E20B6723E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD67789.1.
PIRiT29191.
RefSeqiNP_491237.1. NM_058836.5.

Genome annotation databases

EnsemblMetazoaiT03F1.5.1; T03F1.5.1; WBGene00020187.
T03F1.5.2; T03F1.5.2; WBGene00020187.
GeneIDi171960.
KEGGicel:CELE_T03F1.5.
UCSCiT03F1.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD67789.1.
PIRiT29191.
RefSeqiNP_491237.1. NM_058836.5.

3D structure databases

ProteinModelPortaliP91420.
SMRiP91420. Positions 5-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.T03F1.5.1.

Proteomic databases

EPDiP91420.
PaxDbiP91420.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT03F1.5.1; T03F1.5.1; WBGene00020187.
T03F1.5.2; T03F1.5.2; WBGene00020187.
GeneIDi171960.
KEGGicel:CELE_T03F1.5.
UCSCiT03F1.5. c. elegans.

Organism-specific databases

CTDi171960.
WormBaseiT03F1.5; CE13104; WBGene00020187; gsp-4.

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
InParanoidiP91420.
KOiK01090.
OMAiHETPAIN.
PhylomeDBiP91420.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.
  2. "Sperm chromatin proteomics identifies evolutionarily conserved fertility factors."
    Chu D.S., Liu H., Nix P., Wu T.F., Ralston E.J., Yates J.R. III, Meyer B.J.
    Nature 443:101-105(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  3. "Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans."
    Wu J.C., Go A.C., Samson M., Cintra T., Mirsoian S., Wu T.F., Jow M.M., Routman E.J., Chu D.S.
    Genetics 190:143-157(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiGLC7D_CAEEL
AccessioniPrimary (citable) accession number: P91420
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 8, 2016
Last sequence update: May 1, 1997
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Caution

Due to the high similarity between gsp-3 and gsp-4, the antibody used to study expression does not distinguish between the two proteins.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.