Reviewed, UniProtKB/Swiss-Prot P91268 (PAL_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase F21F3.1
    EC=4.3.2.5
Alternative name(s):
    Probable peptidylamidoglycolate lyase
      Short name=PAL

Gene names

ORF Names:

F21F3.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable lyase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Mediates the dismutation of the unstable peptidyl(2-hydroxyglycine) intermediate to glyoxylate and the corresponding desglycine peptide amide. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity By similarity.
Catalytic activity	Peptidylamidoglycolate = peptidyl amide + glyoxylate.
Cofactor	Zinc By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family. Contains 4 NHL repeats.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro peptidylamidoglycolate lyase activity Inferred from electronic annotation. Source: EC peptidylglycine monooxygenase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 350	331	Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase F21F3.1		PRO_0000248572
Regions
	Repeat	46 – 90	45	NHL 1
	Repeat	113 – 154	42	NHL 2
	Repeat	162 – 206	45	NHL 3
	Repeat	212 – 256	45	NHL 4
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Ref.2
	Disulfide bond	176 ↔ 196		By similarity
	Disulfide bond	241 ↔ 252		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P91268-1 [UniParc]. Last modified March 1, 2003. Version 2. Checksum: A1892DDAAF0756DB	FASTA	350	38,593
	10 20 30 40 50 60 MRASTACLVA LLAPFYISAL PVEYFYGDEQ QPIEEGAENS AVFEQDRELI GLFNPSKEIG 70 80 90 100 110 120 QVSGLAVNKN GHIVAFHRSG RVWDEKSFND HETFNKDLGV INNKTIAIIS REKKVIDEFG 130 140 150 160 170 180 AGLFYMPHGL TIDNNGDYWV TDVGSHQVHK IDAKTQKIVM SLGEKMVPGE DQAHFCKPTD 190 200 210 220 230 240 VAVAKNGHIF VADGYCNSRI LKFDAKGNLM AQINAATEEN QPSEFVVPHS LSLIEDMNIV 250 260 270 280 290 300 CVADRENQRV QCFSAGLSEG DRTLPTGIPI TSATDIGRVF AIREREHYLI GVTGNSEDVE 310 320 330 340 350 AQMFSIDMQT GKTETFAKGV RNTHALAIAA DGVMFVSQLE PSRILEIRLL

References

[1]

"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

[2]

"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-103, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U88175 Genomic DNA. Translation: AAB42278.2.
PIR	T25723.
RefSeq	NP_491475.2.
UniGene	Cel.36441
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	F21F3.1. Caenorhabditis elegans. [Contig view]
GeneID	172108.
KEGG	cel:F21F3.1.
NMPDR	fig|6239.3.peg.1034.
Organism-specific databases
WormBase	WBGene00017671. F21F3.1.
WormPep	F21F3.1. CE32870. [WorfDB]
Phylogenomic databases
OMA	P91268. GFTIDPR.
Enzyme and pathway databases
BRENDA	4.3.2.5. 672.
Gene expression databases
ArrayExpress	P91268.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR001258. NHL_repeat. IPR013017. NHL_repeat_subgr. IPR000720. Pep_amidat_mOase. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
Pfam	PF01436. NHL. 3 hits. [Graphical view]
PRINTS	PR00790. PAMONOXGNASE.
PROSITE	PS51125. NHL. 4 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	874043.

Entry information

Entry name

PAL_CAEEL

Accession

Primary (citable) accession number: P91268

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project