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Protein

Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase pgal-1

Gene

pgal-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable lyase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Mediates the dismutation of the unstable peptidyl(2-hydroxyglycine) intermediate to glyoxylate and the corresponding desglycine peptide amide. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity (By similarity).By similarity

Catalytic activityi

Peptidylamidoglycolate = peptidyl amide + glyoxylate.

Cofactori

Zn2+By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase pgal-1Curated (EC:4.3.2.5)
Alternative name(s):
Probable peptidylamidoglycolate lyase
Short name:
PAL
Gene namesi
Name:pgal-1Imported
ORF Names:F21F3.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF21F3.1; CE32870; WBGene00017671; pgal-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 350331Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase pgal-1CuratedPRO_0000248572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi103 – 1031N-linked (GlcNAc...)1 Publication
Disulfide bondi176 ↔ 196By similarity
Disulfide bondi241 ↔ 252By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP91268.
PaxDbiP91268.

Interactioni

Protein-protein interaction databases

STRINGi6239.F21F3.1.1.

Structurei

3D structure databases

ProteinModelPortaliP91268.
SMRiP91268. Positions 60-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati46 – 9045NHL 1Add
BLAST
Repeati113 – 15442NHL 2Add
BLAST
Repeati162 – 20645NHL 3Add
BLAST
Repeati212 – 25645NHL 4Add
BLAST

Sequence similaritiesi

Contains 4 NHL repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3567. Eukaryota.
ENOG410XS0X. LUCA.
GeneTreeiENSGT00730000111058.
HOGENOMiHOG000045364.
InParanoidiP91268.
KOiK18200.
OMAiHAITLLE.
OrthoDBiEOG70ZZNT.
PhylomeDBiP91268.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR000720. PHM/PAL.
[Graphical view]
PfamiPF01436. NHL. 3 hits.
[Graphical view]
PRINTSiPR00790. PAMONOXGNASE.
PROSITEiPS51125. NHL. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P91268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRASTACLVA LLAPFYISAL PVEYFYGDEQ QPIEEGAENS AVFEQDRELI
60 70 80 90 100
GLFNPSKEIG QVSGLAVNKN GHIVAFHRSG RVWDEKSFND HETFNKDLGV
110 120 130 140 150
INNKTIAIIS REKKVIDEFG AGLFYMPHGL TIDNNGDYWV TDVGSHQVHK
160 170 180 190 200
IDAKTQKIVM SLGEKMVPGE DQAHFCKPTD VAVAKNGHIF VADGYCNSRI
210 220 230 240 250
LKFDAKGNLM AQINAATEEN QPSEFVVPHS LSLIEDMNIV CVADRENQRV
260 270 280 290 300
QCFSAGLSEG DRTLPTGIPI TSATDIGRVF AIREREHYLI GVTGNSEDVE
310 320 330 340 350
AQMFSIDMQT GKTETFAKGV RNTHALAIAA DGVMFVSQLE PSRILEIRLL
Length:350
Mass (Da):38,593
Last modified:March 1, 2003 - v2
Checksum:iA1892DDAAF0756DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081197 Genomic DNA. Translation: CCD69816.1.
PIRiT25723.
RefSeqiNP_491475.2. NM_059074.6.
UniGeneiCel.36441.

Genome annotation databases

EnsemblMetazoaiF21F3.1.1; F21F3.1.1; WBGene00017671.
F21F3.1.2; F21F3.1.2; WBGene00017671.
GeneIDi172108.
KEGGicel:CELE_F21F3.1.
UCSCiF21F3.1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081197 Genomic DNA. Translation: CCD69816.1.
PIRiT25723.
RefSeqiNP_491475.2. NM_059074.6.
UniGeneiCel.36441.

3D structure databases

ProteinModelPortaliP91268.
SMRiP91268. Positions 60-349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F21F3.1.1.

Proteomic databases

EPDiP91268.
PaxDbiP91268.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF21F3.1.1; F21F3.1.1; WBGene00017671.
F21F3.1.2; F21F3.1.2; WBGene00017671.
GeneIDi172108.
KEGGicel:CELE_F21F3.1.
UCSCiF21F3.1.1. c. elegans.

Organism-specific databases

CTDi172108.
WormBaseiF21F3.1; CE32870; WBGene00017671; pgal-1.

Phylogenomic databases

eggNOGiKOG3567. Eukaryota.
ENOG410XS0X. LUCA.
GeneTreeiENSGT00730000111058.
HOGENOMiHOG000045364.
InParanoidiP91268.
KOiK18200.
OMAiHAITLLE.
OrthoDBiEOG70ZZNT.
PhylomeDBiP91268.

Miscellaneous databases

PROiP91268.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR000720. PHM/PAL.
[Graphical view]
PfamiPF01436. NHL. 3 hits.
[Graphical view]
PRINTSiPR00790. PAMONOXGNASE.
PROSITEiPS51125. NHL. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-103, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiPGAL_CAEEL
AccessioniPrimary (citable) accession number: P91268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.