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P91268 (PAL_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase F21F3.1

EC=4.3.2.5
Alternative name(s):
Probable peptidylamidoglycolate lyase
Short name=PAL
Gene names
ORF Names:F21F3.1
OrganismCaenorhabditis elegans
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable lyase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Mediates the dismutation of the unstable peptidyl(2-hydroxyglycine) intermediate to glyoxylate and the corresponding desglycine peptide amide. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity By similarity.

Catalytic activity

Peptidylamidoglycolate = peptidyl amide + glyoxylate.

Cofactor

Zinc By similarity.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.

Contains 4 NHL repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 350331Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase F21F3.1
PRO_0000248572

Regions

Repeat46 – 9045NHL 1
Repeat113 – 15442NHL 2
Repeat162 – 20645NHL 3
Repeat212 – 25645NHL 4

Amino acid modifications

Glycosylation1031N-linked (GlcNAc...) Ref.2
Disulfide bond176 ↔ 196 By similarity
Disulfide bond241 ↔ 252 By similarity

Sequences

Sequence LengthMass (Da)Tools
P91268 [UniParc].

Last modified March 1, 2003. Version 2.
Checksum: A1892DDAAF0756DB

FASTA35038,593
        10         20         30         40         50         60 
MRASTACLVA LLAPFYISAL PVEYFYGDEQ QPIEEGAENS AVFEQDRELI GLFNPSKEIG 

        70         80         90        100        110        120 
QVSGLAVNKN GHIVAFHRSG RVWDEKSFND HETFNKDLGV INNKTIAIIS REKKVIDEFG 

       130        140        150        160        170        180 
AGLFYMPHGL TIDNNGDYWV TDVGSHQVHK IDAKTQKIVM SLGEKMVPGE DQAHFCKPTD 

       190        200        210        220        230        240 
VAVAKNGHIF VADGYCNSRI LKFDAKGNLM AQINAATEEN QPSEFVVPHS LSLIEDMNIV 

       250        260        270        280        290        300 
CVADRENQRV QCFSAGLSEG DRTLPTGIPI TSATDIGRVF AIREREHYLI GVTGNSEDVE 

       310        320        330        340        350 
AQMFSIDMQT GKTETFAKGV RNTHALAIAA DGVMFVSQLE PSRILEIRLL 

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-103, MASS SPECTROMETRY.
Strain: Bristol N2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO081197 Genomic DNA. Translation: CCD69816.1.
PIRT25723.
RefSeqNP_491475.2. NM_059074.5.
UniGeneCel.36441.

3D structure databases

ProteinModelPortalP91268.
SMRP91268. Positions 54-349.
ModBaseSearch...

Protein-protein interaction databases

STRINGP91268.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF21F3.1.1; F21F3.1.1; F21F3.1.
F21F3.1.2; F21F3.1.2; F21F3.1.
GeneID172108.
KEGGcel:F21F3.1.
NMPDRfig|6239.3.peg.1034.
UCSCF21F3.1.1. c. elegans.

Organism-specific databases

CTD172108.
WormBaseF21F3.1; CE32870; WBGene00017671.

Phylogenomic databases

eggNOGmeNOG07371.
GeneTreeEMGT00050000007482.
HOGENOMHBG046476.
InParanoidP91268.
OMAVRGFTID.
PhylomeDBP91268.

Gene expression databases

ArrayExpressP91268.

Family and domain databases

InterProIPR011042. 6-blade_b-propeller_TolB-like.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR000720. Pep_amidat_mOase.
[Graphical view]
Gene3DG3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
PfamPF01436. NHL. 3 hits.
[Graphical view]
PRINTSPR00790. PAMONOXGNASE.
PROSITEPS51125. NHL. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio874043.

Entry information

Entry namePAL_CAEEL
AccessionPrimary (citable) accession number: P91268
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2003
Last modified: December 14, 2011
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families