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Protein

Zinc metalloproteinase nas-9

Gene

nas-9

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Essential metalloprotease.1 Publication

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi401 – 4011Zinc; catalyticBy similarity
Active sitei402 – 4021By similarity
Metal bindingi405 – 4051Zinc; catalyticBy similarity
Metal bindingi411 – 4111Zinc; catalyticBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.A22.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-9 (EC:3.4.24.21)
Alternative name(s):
Nematode astacin 9
Gene namesi
Name:nas-9
ORF Names:C37H5.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC37H5.9a; CE08632; WBGene00003528; nas-9.
C37H5.9b; CE29710; WBGene00003528; nas-9.
C37H5.9c; CE32825; WBGene00003528; nas-9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Defects lead to embryonic lethality in 6% of population.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414Sequence analysisAdd
BLAST
Chaini15 – 546532Zinc metalloproteinase nas-9PRO_0000028914Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence analysis
Disulfide bondi510 ↔ 546PROSITE-ProRule annotation
Disulfide bondi517 ↔ 539PROSITE-ProRule annotation
Disulfide bondi526 ↔ 543PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP91137.
PaxDbiP91137.
PRIDEiP91137.

Interactioni

Protein-protein interaction databases

STRINGi6239.C37H5.9b.

Structurei

3D structure databases

ProteinModelPortaliP91137.
SMRiP91137. Positions 319-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini510 – 54637ShKTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 9270Gly-richAdd
BLAST
Compositional biasi289 – 2968Poly-Gly

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000018720.
InParanoidiP91137.
KOiK08076.
OMAiVAMHETM.
OrthoDBiEOG7VMP8W.
PhylomeDBiP91137.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR017368. Peptidase_M12A_astacin-9/10/11.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PIRSFiPIRSF038055. Nas9/Nas10/Nas11. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS51670. SHKT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b (identifier: P91137-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIFLLFVVFP FVYAQLLPEL LAGFQNGRFR GGPDGFNRGP GGFHRGPDGF
60 70 80 90 100
GGDPRGGVDL GHLIGNIAAN VGQEMGLNDA DVIGDLRGIS RGPRPSSMEW
110 120 130 140 150
GRRARHFCRR YPGHPKCQRG QLPQFTDVPT IINTIIYNAG DLLPRVPTLN
160 170 180 190 200
IHDPLAGLNS ELVGFIKSLQ SQFGQLSSQQ RNEIHDSCRS FKCDQQSPQN
210 220 230 240 250
TQAKQELLTK MLAFDQAVGG KAAPAHDKVN LRFDRTQQVK QALLKRANLS
260 270 280 290 300
HIIVPADNGV FDRDVLLTEH QANFLLNELG EAGRGADVGA GGGGGGRVPR
310 320 330 340 350
SGVFFQESAV QKWDIWKPIQ YTLDDSLEES DKKDIRDALH EISINTCILF
360 370 380 390 400
RYNATPKGYH LNYMKVDSTT FCGLSYVGRT DPANPIYLSF QCGDNRGVAM
410 420 430 440 450
HETMHALGVS HQHLRLDRDK YIKIDWSNID PQHYDTFAIS DAKLYTSYGT
460 470 480 490 500
KYAYDSIMHY NAYLGAKDPN KPTMIPLVNP QENTPKLGQR AKLTRGDIRL
510 520 530 540
LKKMYCRPGC DDQNVHCGTW ALHGYCKMKE QMKWMNENCK ASCDKC
Length:546
Mass (Da):60,977
Last modified:January 4, 2005 - v2
Checksum:iEAB496C2E69AA476
GO
Isoform a (identifier: P91137-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     395-423: Missing.

Note: No experimental confirmation available.
Show »
Length:517
Mass (Da):57,581
Checksum:i8D966934D68648BF
GO
Isoform c (identifier: P91137-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Note: No experimental confirmation available.
Show »
Length:472
Mass (Da):53,166
Checksum:i4A8D1F82B9AF5FD6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7474Missing in isoform c. CuratedVSP_012352Add
BLAST
Alternative sequencei395 – 42329Missing in isoform a. CuratedVSP_012353Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080813 Genomic DNA. Translation: CCD66988.1.
FO080813 Genomic DNA. Translation: CCD66989.1.
FO080813 Genomic DNA. Translation: CCD66990.1.
PIRiT25615.
RefSeqiNP_504293.2. NM_071892.8. [P91137-3]
NP_741531.1. NM_171452.1. [P91137-2]
NP_741532.1. NM_171927.1. [P91137-1]
UniGeneiCel.8793.

Genome annotation databases

EnsemblMetazoaiC37H5.9b; C37H5.9b; WBGene00003528. [P91137-1]
GeneIDi178875.
KEGGicel:CELE_C37H5.9.
UCSCiC37H5.9c.1. c. elegans. [P91137-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080813 Genomic DNA. Translation: CCD66988.1.
FO080813 Genomic DNA. Translation: CCD66989.1.
FO080813 Genomic DNA. Translation: CCD66990.1.
PIRiT25615.
RefSeqiNP_504293.2. NM_071892.8. [P91137-3]
NP_741531.1. NM_171452.1. [P91137-2]
NP_741532.1. NM_171927.1. [P91137-1]
UniGeneiCel.8793.

3D structure databases

ProteinModelPortaliP91137.
SMRiP91137. Positions 319-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C37H5.9b.

Protein family/group databases

MEROPSiM12.A22.

Proteomic databases

EPDiP91137.
PaxDbiP91137.
PRIDEiP91137.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC37H5.9b; C37H5.9b; WBGene00003528. [P91137-1]
GeneIDi178875.
KEGGicel:CELE_C37H5.9.
UCSCiC37H5.9c.1. c. elegans. [P91137-1]

Organism-specific databases

CTDi178875.
WormBaseiC37H5.9a; CE08632; WBGene00003528; nas-9.
C37H5.9b; CE29710; WBGene00003528; nas-9.
C37H5.9c; CE32825; WBGene00003528; nas-9.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000018720.
InParanoidiP91137.
KOiK08076.
OMAiVAMHETM.
OrthoDBiEOG7VMP8W.
PhylomeDBiP91137.

Miscellaneous databases

PROiP91137.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR017368. Peptidase_M12A_astacin-9/10/11.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PIRSFiPIRSF038055. Nas9/Nas10/Nas11. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS51670. SHKT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "The astacin protein family in Caenorhabditis elegans."
    Moehrlen F., Hutter H., Zwilling R.
    Eur. J. Biochem. 270:4909-4920(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NOMENCLATURE, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiNAS9_CAEEL
AccessioniPrimary (citable) accession number: P91137
Secondary accession number(s): Q8I7H6, Q95Q69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: June 8, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.