P91134 (PURA_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4

Alternative name(s):
IMP--aspartate ligase

Gene names

ORF Names:

C37H5.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity.
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative initiation
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase negative regulation of vulval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW receptor-mediated endocytosis Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform a (identifier: P91134-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P91134-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Adenylosuccinate synthetase		PRO_0000095134
Regions
	Nucleotide binding	40 – 46	7	GTP By similarity
	Nucleotide binding	70 – 72	3	GTP By similarity
	Nucleotide binding	362 – 364	3	GTP By similarity
	Nucleotide binding	444 – 446	3	GTP By similarity
	Region	41 – 44	4	IMP binding By similarity
	Region	68 – 71	4	IMP binding By similarity
	Region	330 – 336	7	Substrate binding By similarity
Sites
	Active site	41	1	Proton acceptor By similarity
	Active site	71	1	Proton donor By similarity
	Metal binding	41	1	Magnesium By similarity
	Metal binding	70	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	161	1	IMP By similarity
	Binding site	175	1	IMP; shared with dimeric partner By similarity
	Binding site	255	1	IMP By similarity
	Binding site	270	1	IMP By similarity
	Binding site	334	1	IMP By similarity
	Binding site	336	1	GTP By similarity
Natural variations
	Alternative sequence	1 – 23	23	Missing in isoform b.		VSP_039823

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified October 5, 2010. Version 3. Checksum: 3B2B7D5A11925E3E	FASTA	457	50,161
	10 20 30 40 50 60 MGSVTSKTND ELESFLNKRL GGSMTSSNKT VSVLLGAQWG DEGKGKIIDY LIENHKINVT 70 80 90 100 110 120 ARCQGGNNAG HTVVANGRKY DFHILPSGII SPTCFNVIGN GVVVNLDAFF SELAHNGILE 130 140 150 160 170 180 ESGWEKRIMI SSEAHLVFGV HSQVDGRQED SLAAKNKIGT TNRGIGPTYS SKCFRNGIRV 190 200 210 220 230 240 ADLMADFEEF SEKYRRLVEH YKKQFPSIEV NVDEELAKFK QHREKLAELK LVGDTVGFIH 250 260 270 280 290 300 EQRNAGKQVL VEGANGALLD IDFGTYPYVT SSNSTVGGAC TGIGVPPTAV GNVIGVVKAY 310 320 330 340 350 360 QTRVGTGPFP TELFDSDGEK LQTIGKEVGV TTGRKRRCGW IDLFLLRRSA MINGYTAIAL 370 380 390 400 410 420 TKLDILDTFP TIKVAVGYKL NGQVLSSPPA QANAWGAIEV EYKEFEGWNE PTVGVRKFED 430 440 450 LPEKCRQYVK FIEDFIKVPI VYIGVGAERE SLIVRQQ
	Isoform b [UniParc]. Checksum: E9FFEB5E3F2E784F	FASTA	434	47,708
	10 20 30 40 50 60 MTSSNKTVSV LLGAQWGDEG KGKIIDYLIE NHKINVTARC QGGNNAGHTV VANGRKYDFH 70 80 90 100 110 120 ILPSGIISPT CFNVIGNGVV VNLDAFFSEL AHNGILEESG WEKRIMISSE AHLVFGVHSQ 130 140 150 160 170 180 VDGRQEDSLA AKNKIGTTNR GIGPTYSSKC FRNGIRVADL MADFEEFSEK YRRLVEHYKK 190 200 210 220 230 240 QFPSIEVNVD EELAKFKQHR EKLAELKLVG DTVGFIHEQR NAGKQVLVEG ANGALLDIDF 250 260 270 280 290 300 GTYPYVTSSN STVGGACTGI GVPPTAVGNV IGVVKAYQTR VGTGPFPTEL FDSDGEKLQT 310 320 330 340 350 360 IGKEVGVTTG RKRRCGWIDL FLLRRSAMIN GYTAIALTKL DILDTFPTIK VAVGYKLNGQ 370 380 390 400 410 420 VLSSPPAQAN AWGAIEVEYK EFEGWNEPTV GVRKFEDLPE KCRQYVKFIE DFIKVPIVYI 430 GVGAERESLI VRQQ

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080813 Genomic DNA. Translation: CCD66985.1. FO080813 Genomic DNA. Translation: CCD66986.1.
PIR	T25612.
RefSeq	NP_741529.1. NM_171450.1. NP_741530.1. NM_171451.2.
UniGene	Cel.17687.
3D structure databases
ProteinModelPortal	P91134.
SMR	P91134. Positions 25-455.
ModBase	Search...
Protein-protein interaction databases
STRING	P91134.
Proteomic databases
PRIDE	P91134.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C37H5.6a; C37H5.6a; C37H5.6.
GeneID	178872.
KEGG	cel:C37H5.6.
UCSC	C37H5.6b.1. c. elegans.
Organism-specific databases
CTD	178872.
WormBase	C37H5.6a; CE08630; WBGene00016509. C37H5.6b; CE29709; WBGene00016509.
Phylogenomic databases
eggNOG	meNOG04288.
GeneTree	EMGT00050000011166.
OMA	YVLGIIK.
PhylomeDB	P91134.
Gene expression databases
ArrayExpress	P91134.
Family and domain databases
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
KO	K01939.
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. PurA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	902916.

Entry information

Entry name

PURA_CAEEL

Accession

Primary (citable) accession number: P91134
Secondary accession number(s): Q8MNS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 5, 2010
Last modified:	December 14, 2011
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project