P91119 (PDE5_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3',5'-cyclic phosphodiesterase pde-5
EC=3.1.4.17

Gene names

Name:	pde-5
ORF Names:	C32E12.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 1 GAF domain.

Ontologies

Keywords
Domain	Coiled coil
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	reproduction Inferred from mutant phenotype. Source: WormBase signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Probable 3',5'-cyclic phosphodiesterase pde-5		PRO_0000198848
Regions
	Domain	214 – 371	158	GAF
	Coiled coil	691 – 728	38	Potential
Sites
	Active site	465	1	Proton donor By similarity
	Metal binding	469	1	Divalent metal cation 1 By similarity
	Metal binding	503	1	Divalent metal cation 1 By similarity
	Metal binding	504	1	Divalent metal cation 1 By similarity
	Metal binding	504	1	Divalent metal cation 2 By similarity
	Metal binding	614	1	Divalent metal cation 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P91119 [UniParc]. Last modified October 19, 2011. Version 3. Checksum: 7C28EC4EEEC16D85	FASTA	728	83,227
	10 20 30 40 50 60 MDDASVLKYL QENPKLVEDF VVSNEISPET FKRWAVRRTM KYKNVKNGTS GGTGAWTEPD 70 80 90 100 110 120 LSMKRRVILE TSDNRTRILY EITQCCGQLI GTNSIELIVQ NDEGAFSCRK TENGELKLKK 130 140 150 160 170 180 VKTSKSADYI QTIVNAGNQT IAEIHFYTQL DSTEKSIVNA VCTWAAATNY YSELYTHKQE 190 200 210 220 230 240 GSDGQDIHEN IAKQRKLSNF LLDVARSIFH DIVSMDAVII KVMNFAQKLV DADRASLFLV 250 260 270 280 290 300 DSKNAQIYAR IFDVGTGDEE HVRVNSEGQK EIRFDMSKGI AGYVASTGEG LNIENAYEDE 310 320 330 340 350 360 RFNADVDSKT GYTTKTILCM PILIRGIVIG VVQMVNKHDG VFTRQDEDAF EIFAVYCGLA 370 380 390 400 410 420 LHHAKLYDKI RRSEQKYRVA LEVLAYHSVC NADEVNKLKK IEINNRIVEL ETIDFNGMRL 430 440 450 460 470 480 SELEKPLYAV YMFKTLFADT LRFDTEDLIR FVLTVRKNYR RVAYHNWAHG WSVAHAMFAT 490 500 510 520 530 540 LMNSPDAFTK LEALALYVSC LCHDLDHRGK NNAYMKTMST PLASIYSTSV MERHHFNQTV 550 560 570 580 590 600 TILQQDGHNI LKSLSSEDYK KTLSLIKHCI LATDLALFFS NKAKLNVILD NNTFDINRQE 610 620 630 640 650 660 HRLLTQAVMM TGCDLVASAK PWNIQTETVK VIFEEFYDQG DAERLSGKEP IPMMDRQQAH 670 680 690 700 710 720 MLPQMQVGFM RGICMPCYDL IARIFPKNDK MRERCEYNAK KWEELAEEQR KKQEALAQQN GEANETQE

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080144 Genomic DNA. Translation: CCD61583.1. FO080752 Genomic DNA. Translation: CCD66434.1.
PIR	T25590.
UniGene	Cel.19170.
3D structure databases
ProteinModelPortal	P91119.
ModBase	Search...
Protein-protein interaction databases
STRING	P91119.
Proteomic databases
PRIDE	P91119.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	C32E12.2. c. elegans.
Organism-specific databases
CTD	183125.
WormBase	C32E12.2; CE45692; WBGene00016328; pde-5.
Phylogenomic databases
eggNOG	meNOG04250.
GeneTree	EMGT00050000002162.
HOGENOM	HBG378345.
InParanoid	P91119.
PhylomeDB	P91119.
Gene expression databases
ArrayExpress	P91119.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 1 hit.
Pfam	PF01590. GAF. 1 hit. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 1 hit. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	920014.

Entry information

Entry name

PDE5_CAEEL

Accession

Primary (citable) accession number: P91119

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	October 19, 2011
Last modified:	January 25, 2012
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project