P91119 (PDE5_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3',5'-cyclic phosphodiesterase pde-5
EC=3.1.4.17

Gene names

Name:	pde-5
ORF Names:	C32E12.2

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 1 GAF domain.

Ontologies

Keywords
Domain	Coiled coil
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Probable 3',5'-cyclic phosphodiesterase pde-5		PRO_0000198848
Regions
	Domain	214 – 371	158	GAF
	Coiled coil	691 – 728	38	Potential
Sites
	Active site	465	1	Proton donor By similarity
	Metal binding	469	1	Divalent metal cation 1 By similarity
	Metal binding	503	1	Divalent metal cation 1 By similarity
	Metal binding	504	1	Divalent metal cation 1 By similarity
	Metal binding	504	1	Divalent metal cation 2 By similarity
	Metal binding	614	1	Divalent metal cation 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P91119 [UniParc]. Last modified October 19, 2011. Version 3. Checksum: 7C28EC4EEEC16D85	FASTA	728	83,227
	10 20 30 40 50 60 MDDASVLKYL QENPKLVEDF VVSNEISPET FKRWAVRRTM KYKNVKNGTS GGTGAWTEPD 70 80 90 100 110 120 LSMKRRVILE TSDNRTRILY EITQCCGQLI GTNSIELIVQ NDEGAFSCRK TENGELKLKK 130 140 150 160 170 180 VKTSKSADYI QTIVNAGNQT IAEIHFYTQL DSTEKSIVNA VCTWAAATNY YSELYTHKQE 190 200 210 220 230 240 GSDGQDIHEN IAKQRKLSNF LLDVARSIFH DIVSMDAVII KVMNFAQKLV DADRASLFLV 250 260 270 280 290 300 DSKNAQIYAR IFDVGTGDEE HVRVNSEGQK EIRFDMSKGI AGYVASTGEG LNIENAYEDE 310 320 330 340 350 360 RFNADVDSKT GYTTKTILCM PILIRGIVIG VVQMVNKHDG VFTRQDEDAF EIFAVYCGLA 370 380 390 400 410 420 LHHAKLYDKI RRSEQKYRVA LEVLAYHSVC NADEVNKLKK IEINNRIVEL ETIDFNGMRL 430 440 450 460 470 480 SELEKPLYAV YMFKTLFADT LRFDTEDLIR FVLTVRKNYR RVAYHNWAHG WSVAHAMFAT 490 500 510 520 530 540 LMNSPDAFTK LEALALYVSC LCHDLDHRGK NNAYMKTMST PLASIYSTSV MERHHFNQTV 550 560 570 580 590 600 TILQQDGHNI LKSLSSEDYK KTLSLIKHCI LATDLALFFS NKAKLNVILD NNTFDINRQE 610 620 630 640 650 660 HRLLTQAVMM TGCDLVASAK PWNIQTETVK VIFEEFYDQG DAERLSGKEP IPMMDRQQAH 670 680 690 700 710 720 MLPQMQVGFM RGICMPCYDL IARIFPKNDK MRERCEYNAK KWEELAEEQR KKQEALAQQN GEANETQE

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080144 Genomic DNA. Translation: CCD61583.1.
PIR	T25590.
RefSeq	NP_491544.3. NM_059143.3.
3D structure databases
ProteinModelPortal	P91119.
SMR	P91119. Positions 194-709.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.C32E12.2.
Proteomic databases
PaxDb	P91119.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C32E12.2; C32E12.2; C32E12.2.
GeneID	183125.
KEGG	cel:CELE_C32E12.2.
UCSC	C32E12.2. c. elegans.
Organism-specific databases
CTD	183125.
WormBase	C32E12.2; CE45692; WBGene00016328; pde-5.
Phylogenomic databases
eggNOG	NOG270709.
GeneTree	ENSGT00550000074280.
HOGENOM	HOG000007068.
InParanoid	P91119.
OMA	HENIAKQ.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003018. GAF. IPR003607. HD/PDEase_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Pfam	PF01590. GAF. 1 hit. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 1 hit. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	920014.

Entry information

Entry name

PDE5_CAEEL

Accession

Primary (citable) accession number: P91119

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	October 19, 2011
Last modified:	May 1, 2013
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project