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P91119 (PDE5_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 3',5'-cyclic phosphodiesterase pde-5

EC=3.1.4.17
Gene names
Name:pde-5
ORF Names:C32E12.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length728 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 1 GAF domain.

Ontologies

Keywords
   DomainCoiled coil
   LigandcGMP
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsignal transduction

Inferred from electronic annotation. Source: InterPro

   Molecular_function3',5'-cyclic-nucleotide phosphodiesterase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 728728Probable 3',5'-cyclic phosphodiesterase pde-5
PRO_0000198848

Regions

Domain214 – 371158GAF
Coiled coil691 – 72838 Potential

Sites

Active site4651Proton donor By similarity
Metal binding4691Divalent metal cation 1 By similarity
Metal binding5031Divalent metal cation 1 By similarity
Metal binding5041Divalent metal cation 1 By similarity
Metal binding5041Divalent metal cation 2 By similarity
Metal binding6141Divalent metal cation 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
P91119 [UniParc].

Last modified October 19, 2011. Version 3.
Checksum: 7C28EC4EEEC16D85

FASTA72883,227
        10         20         30         40         50         60 
MDDASVLKYL QENPKLVEDF VVSNEISPET FKRWAVRRTM KYKNVKNGTS GGTGAWTEPD 

        70         80         90        100        110        120 
LSMKRRVILE TSDNRTRILY EITQCCGQLI GTNSIELIVQ NDEGAFSCRK TENGELKLKK 

       130        140        150        160        170        180 
VKTSKSADYI QTIVNAGNQT IAEIHFYTQL DSTEKSIVNA VCTWAAATNY YSELYTHKQE 

       190        200        210        220        230        240 
GSDGQDIHEN IAKQRKLSNF LLDVARSIFH DIVSMDAVII KVMNFAQKLV DADRASLFLV 

       250        260        270        280        290        300 
DSKNAQIYAR IFDVGTGDEE HVRVNSEGQK EIRFDMSKGI AGYVASTGEG LNIENAYEDE 

       310        320        330        340        350        360 
RFNADVDSKT GYTTKTILCM PILIRGIVIG VVQMVNKHDG VFTRQDEDAF EIFAVYCGLA 

       370        380        390        400        410        420 
LHHAKLYDKI RRSEQKYRVA LEVLAYHSVC NADEVNKLKK IEINNRIVEL ETIDFNGMRL 

       430        440        450        460        470        480 
SELEKPLYAV YMFKTLFADT LRFDTEDLIR FVLTVRKNYR RVAYHNWAHG WSVAHAMFAT 

       490        500        510        520        530        540 
LMNSPDAFTK LEALALYVSC LCHDLDHRGK NNAYMKTMST PLASIYSTSV MERHHFNQTV 

       550        560        570        580        590        600 
TILQQDGHNI LKSLSSEDYK KTLSLIKHCI LATDLALFFS NKAKLNVILD NNTFDINRQE 

       610        620        630        640        650        660 
HRLLTQAVMM TGCDLVASAK PWNIQTETVK VIFEEFYDQG DAERLSGKEP IPMMDRQQAH 

       670        680        690        700        710        720 
MLPQMQVGFM RGICMPCYDL IARIFPKNDK MRERCEYNAK KWEELAEEQR KKQEALAQQN 


GEANETQE 

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References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO080144 Genomic DNA. Translation: CCD61583.1.
PIRT25590.
RefSeqNP_491544.3. NM_059143.3.
UniGeneCel.19170.

3D structure databases

ProteinModelPortalP91119.
SMRP91119. Positions 74-709.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6239.C32E12.2.

Proteomic databases

PaxDbP91119.
PRIDEP91119.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC32E12.2; C32E12.2; C32E12.2.
GeneID183125.
KEGGcel:CELE_C32E12.2.
UCSCC32E12.2. c. elegans.

Organism-specific databases

CTD183125.
WormBaseC32E12.2; CE45692; WBGene00016328; pde-5.

Phylogenomic databases

eggNOGNOG270709.
HOGENOMHOG000007068.
InParanoidP91119.
KOK01120.
OMAHENIAKQ.
OrthoDBEOG7WQ7RN.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
InterProIPR003018. GAF.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 1 hit.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 1 hit.
SM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio920014.
PROP91119.

Entry information

Entry namePDE5_CAEEL
AccessionPrimary (citable) accession number: P91119
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 19, 2011
Last modified: April 16, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase