Reviewed, UniProtKB/Swiss-Prot P91119 (PDE5_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable 3',5'-cyclic phosphodiesterase pde-5
    EC=3.1.4.17

Gene names

Name:	pde-5
ORF Names:	C32E12.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	710 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.
Sequence caution	The sequence AAB53879.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	reproduction Inferred from mutant phenotype. Source: WormBase signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 710	710	Probable 3',5'-cyclic phosphodiesterase pde-5		PRO_0000198848
Sites
	Active site	485	1	Proton donor By similarity
	Metal binding	489	1	Divalent metal cation 1 By similarity
	Metal binding	523	1	Divalent metal cation 1 By similarity
	Metal binding	524	1	Divalent metal cation 1 By similarity
	Metal binding	524	1	Divalent metal cation 2 By similarity
	Metal binding	634	1	Divalent metal cation 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P91119-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 508D8916E0C7FF0D	FASTA	710	81,136
	10 20 30 40 50 60 MDDASVLKYL QENPKLVEDF VVSNEISPET FKRWAVRRTM KYKNVKNGTS GGTGAWTEPD 70 80 90 100 110 120 LSMKRRVILE TSDNRTRILY EITQCCGQLI GTNSIELIVQ NDEGAFSCRK TENGELKLKK 130 140 150 160 170 180 VKTSKSADYI QTIVNAGNQT IAEIHFYTQL DSTEKSIVNA VCTWAAATNY YSEVRKLQKH 190 200 210 220 230 240 ITCSFLKPKQ SFQLYTHKQE GSDGQDIHEN IAKQRKLSNF LLDVARSIFH DIVSMDAVII 250 260 270 280 290 300 KVMNFAQKLV DADRASLFLV DSKNAQIYAR IFDVGTGDEE HVRVNSEGQK EIRFDMSKGI 310 320 330 340 350 360 AGYVASTGEG LNIENAYEDE RFNADVDSKT GYTTKTILCM PILIRGIVIG VVQMVNKHDG 370 380 390 400 410 420 VFTRQDEDAF EIFAVYCGLA LHHAKLYDKI RRSEQKYRVA LEVLAYHSVC NADEVNKLKK 430 440 450 460 470 480 IEINNRIVEL ETIDFNGMRL SELEKPLYAV YMFKTLFADT LRFDTEDLIR FVLTVRKNYR 490 500 510 520 530 540 RVAYHNWAHG WSVAHAMFAT LMNSPDAFTK LEALALYVSC LCHDLDHRGK NNAYMKTMST 550 560 570 580 590 600 PLASIYSTSV MERHHFNQTV TILQQDGHNI LKSLSSEDYK KTLSLIKHCI LATDLALFFS 610 620 630 640 650 660 NKAKLNVILD NNTFDINRQE HRLLTQAVMM TGCDLVASAK PWNIQTETVK VIFEEFYDQG 670 680 690 700 710 DAERLSGKEP IPMMDRQQAH MLPQMQASRL HAWDLYALLR LNRANIPEKR

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U80032 Genomic DNA. Translation: AAB53879.2. Sequence problems. U80446 Genomic DNA. No translation available.
PIR	T25590.
RefSeq	NP_491544.2.
UniGene	Cel.19170
3D structure databases
SMR	P91119. Positions 218-382, 428-691.
ModBase	Search...
Protein-protein interaction databases
STRING	P91119.
Genome annotation databases
Ensembl	C32E12.2; C32E12.2; C32E12.2; Caenorhabditis elegans. [Genome view]
GeneID	183125.
KEGG	cel:C32E12.2.
UCSC	C32E12.2. c. elegans.
Organism-specific databases
CTD	183125.
WormBase	WBGene00016328. pde-5.
WormPep	C32E12.2. CE40672. [WorfDB]
Phylogenomic databases
eggNOG	meNOG04250.
HOGENOM	HBG378345.
InParanoid	P91119.
PhylomeDB	P91119.
Enzyme and pathway databases
BRENDA	3.1.4.17. 672.
Gene expression databases
ArrayExpress	P91119.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 1 hit. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 1 hit. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	920014.

Entry information

Entry name

PDE5_CAEEL

Accession

Primary (citable) accession number: P91119

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 10, 2007
Last modified:	February 9, 2010
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project