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Protein

Glutathione-independent glyoxalase DJR-1.1

Gene

djr-1.1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) or glyoxal (GO) to D-lactate or glycolic acid respectively in a single glutathione (GSH)-independent step. May play a role in detoxifying endogenously produced glyoxals. Involved in protection against glyoxal-induced cell death.1 Publication

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.1 Publication

Kineticsi

kcat is 13.8 min(-1) with methylglyoxal as substrate and 356.4 min(-1) with glyoxal as substrate.1 Publication

  1. KM=0.3 mM for methylglyoxal1 Publication
  2. KM=3.61 mM for glyoxal1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei19 – 191By similarity
    Active sitei106 – 1061By similarity
    Active sitei125 – 1251By similarity

    GO - Molecular functioni

    • glyoxalase (glycolic acid-forming) activity Source: ParkinsonsUK-UCL
    • glyoxalase III activity Source: ParkinsonsUK-UCL
    • transcription coactivator activity Source: GO_Central

    GO - Biological processi

    • cellular response to glyoxal Source: ParkinsonsUK-UCL
    • cellular response to hydrogen peroxide Source: GO_Central
    • cellular response to methylglyoxal Source: ParkinsonsUK-UCL
    • glycolate biosynthetic process Source: ParkinsonsUK-UCL
    • glyoxal catabolic process Source: ParkinsonsUK-UCL
    • methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: ParkinsonsUK-UCL
    • negative regulation of neuron death Source: ParkinsonsUK-UCL
    • regulation of transcription from RNA polymerase II promoter Source: GO_Central
    • response to toxic substance Source: WormBase
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione-independent glyoxalase DJR-1.11 Publication (EC:4.2.1.1301 Publication)
    Alternative name(s):
    Protein DJ-1 homolog 1
    Gene namesi
    Name:djr-1.1Imported
    ORF Names:B0432.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    Proteomesi
    • UP000001940 Componenti: Chromosome II

    Organism-specific databases

    WormBaseiB0432.2; CE07740; WBGene00015184; djr-1.1.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: WormBase
    • cytosol Source: GO_Central
    • nucleus Source: WormBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi106 – 1061C → S: Completely abolishes catalytic activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 187187Glutathione-independent glyoxalase DJR-1.1PRO_0000432108Add
    BLAST

    Proteomic databases

    EPDiP90994.
    PaxDbiP90994.

    Expressioni

    Tissue specificityi

    Expressed exclusively in the intestine.1 Publication

    Gene expression databases

    BgeeiWBGene00015184.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-24307N.
    MINTiMINT-1093979.
    STRINGi6239.B0432.2.2.

    Structurei

    3D structure databases

    ProteinModelPortaliP90994.
    SMRiP90994. Positions 4-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C56 family.Curated

    Phylogenomic databases

    eggNOGiKOG2764. Eukaryota.
    COG0693. LUCA.
    GeneTreeiENSGT00390000001231.
    HOGENOMiHOG000063194.
    InParanoidiP90994.
    KOiK05687.
    OMAiAICAAPY.
    OrthoDBiEOG091G12NS.
    PhylomeDBiP90994.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    IPR006287. DJ1.
    [Graphical view]
    PfamiPF01965. DJ-1_PfpI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01383. not_thiJ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P90994-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAQKSALIIL AAEGAEEMEV IITGDVLARG EIRVVYAGLD GAEPVKCARG
    60 70 80 90 100
    AHIVPDVKLE DVETEKFDIV ILPGGQPGSN TLAESLLVRD VLKSQVESGG
    110 120 130 140 150
    LIGAICAAPI ALLSHGVKAE LVTSHPSVKE KLEKGGYKYS EDRVVVSGKI
    160 170 180
    ITSRGPGTAF EFALKIVELL EGKDKATSLI APMLLKL
    Length:187
    Mass (Da):19,669
    Last modified:May 1, 1997 - v1
    Checksum:iA6D124C7E8BB1AF1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    FO080203 Genomic DNA. Translation: CCD61936.1.
    PIRiT25461.
    RefSeqiNP_493696.1. NM_061295.6.
    UniGeneiCel.16048.

    Genome annotation databases

    EnsemblMetazoaiB0432.2; B0432.2; WBGene00015184.
    GeneIDi173416.
    KEGGicel:CELE_B0432.2.
    UCSCiB0432.2. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    FO080203 Genomic DNA. Translation: CCD61936.1.
    PIRiT25461.
    RefSeqiNP_493696.1. NM_061295.6.
    UniGeneiCel.16048.

    3D structure databases

    ProteinModelPortaliP90994.
    SMRiP90994. Positions 4-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-24307N.
    MINTiMINT-1093979.
    STRINGi6239.B0432.2.2.

    Proteomic databases

    EPDiP90994.
    PaxDbiP90994.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblMetazoaiB0432.2; B0432.2; WBGene00015184.
    GeneIDi173416.
    KEGGicel:CELE_B0432.2.
    UCSCiB0432.2. c. elegans.

    Organism-specific databases

    CTDi173416.
    WormBaseiB0432.2; CE07740; WBGene00015184; djr-1.1.

    Phylogenomic databases

    eggNOGiKOG2764. Eukaryota.
    COG0693. LUCA.
    GeneTreeiENSGT00390000001231.
    HOGENOMiHOG000063194.
    InParanoidiP90994.
    KOiK05687.
    OMAiAICAAPY.
    OrthoDBiEOG091G12NS.
    PhylomeDBiP90994.

    Miscellaneous databases

    PROiP90994.

    Gene expression databases

    BgeeiWBGene00015184.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    IPR006287. DJ1.
    [Graphical view]
    PfamiPF01965. DJ-1_PfpI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01383. not_thiJ. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDJ11_CAEEL
    AccessioniPrimary (citable) accession number: P90994
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 4, 2015
    Last sequence update: May 1, 1997
    Last modified: September 7, 2016
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.