Reviewed, UniProtKB/Swiss-Prot P90986 (TY3H_CAEEL)

Names and origin

Protein names

Recommended name:
    Tyrosine 3-monooxygenase
    EC=1.14.16.2
Alternative name(s):
    Tyrosine 3-hydroxylase
      Short name=TH

Gene names

Name:	cat-2
ORF Names:	B0432.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion.
Pathway	Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
Biological process	Catecholamine biosynthesis Neurotransmitter biosynthesis
Coding sequence diversity	Alternative splicing
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family metabolic process Inferred from electronic annotation. Source: InterPro catecholamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform b (identifier: P90986-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confimation available.

Isoform a (identifier: P90986-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MFLRYMRRRGSGEDHEEEPRGVTVIATKVAENSKNPRRYSLVHQASCETQHHKGIRRQNTIQHRKQLTDQM
     324-454: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Tyrosine 3-monooxygenase		PRO_0000205565
Sites
	Metal binding	282	1	Iron By similarity
	Metal binding	287	1	Iron By similarity
	Metal binding	327	1	Iron By similarity
Natural variations
	Alternative sequence	1	1	M → MFLRYMRRRGSGEDHEEEPR GVTVIATKVAENSKNPRRYS LVHQASCETQHHKGIRRQNT IQHRKQLTDQM in isoform a.		VSP_013645
	Alternative sequence	324 – 454	131	Missing in isoform a.		VSP_013646

Sequences

	Sequence		Length	Mass (Da)
	Isoform b [UniParc]. Last modified May 10, 2005. Version 3. Checksum: 1AC61E1D39DBB283	FASTA	454	51,313
	10 20 30 40 50 60 MRCQKILQQL NDEGIEVIFT ANDVTPIEFS IILTSTDPTL SNFVSDILQN MSSAKVQICH 70 80 90 100 110 120 VETRGNEASH DVLLACKATK NQLIHSAELL TQNHVALTKF SIFAKKLSDE KNQSQIWFPR 130 140 150 160 170 180 HISELDQCSK CITKYEPTTD PRHPGHGDVA YIARRKFLND QALEFKFGDE IGYVDYTEEE 190 200 210 220 230 240 HATWKAVYEK LGDLHLSHTC AVYRQNLKIL QEEKVLTADR IPQIRDVNKF LQKKTGFELR 250 260 270 280 290 300 PCSGLLSARD FLASLAFRVF QTTTYLRHHK SPHHSPEPDL IHELLGHVPM FSDPLLAQMS 310 320 330 340 350 360 QDIGLMSLGA SDEHIEKLST VYWFIVEFGL CKEDGKLKAI GAGLLSAYGE LMHACSDAPE 370 380 390 400 410 420 HKDFDPAVTA VQKYEDDDYQ PLYFVADSIH DALAKLRKYA SSMDRPFSVV YDPFTKSIEA 430 440 450 IESSADLEKA FSRLSNDLSA ITHAADRMKI SITM
	Isoform a. Checksum: EB3FFD8636D2D003	FASTA	393	45,123
	10 20 30 40 50 60 MFLRYMRRRG SGEDHEEEPR GVTVIATKVA ENSKNPRRYS LVHQASCETQ HHKGIRRQNT 70 80 90 100 110 120 IQHRKQLTDQ MRCQKILQQL NDEGIEVIFT ANDVTPIEFS IILTSTDPTL SNFVSDILQN 130 140 150 160 170 180 MSSAKVQICH VETRGNEASH DVLLACKATK NQLIHSAELL TQNHVALTKF SIFAKKLSDE 190 200 210 220 230 240 KNQSQIWFPR HISELDQCSK CITKYEPTTD PRHPGHGDVA YIARRKFLND QALEFKFGDE 250 260 270 280 290 300 IGYVDYTEEE HATWKAVYEK LGDLHLSHTC AVYRQNLKIL QEEKVLTADR IPQIRDVNKF 310 320 330 340 350 360 LQKKTGFELR PCSGLLSARD FLASLAFRVF QTTTYLRHHK SPHHSPEPDL IHELLGHVPM 370 380 390 FSDPLLAQMS QDIGLMSLGA SDEHIEKLST VYW

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U80836 Genomic DNA. Translation: AAV58889.1. U80836 Genomic DNA. Translation: AAV58890.1.
PIR	T25453.
RefSeq	NP_493688.3. NP_871903.2.
UniGene	Cel.8024
3D structure databases
SMR	P90986. Positions 116-448.
ModBase	Search...
Protein-protein interaction databases
STRING	P90986.
Proteomic databases
PRIDE	P90986.
Genome annotation databases
Ensembl	B0432.5b; B0432.5b; B0432.5; Caenorhabditis elegans. [Genome view]
GeneID	173411.
KEGG	cel:B0432.5.
UCSC	B0432.5a. c. elegans.
Organism-specific databases
CTD	173411.
WormBase	WBGene00000296. cat-2.
WormPep	B0432.5a. CE32103. [WorfDB] B0432.5b. CE37742. [WorfDB]
Phylogenomic databases
eggNOG	meNOG04044.
HOGENOM	HBG484724.
InParanoid	P90986.
OMA	CSDAPEH.
PhylomeDB	P90986.
Enzyme and pathway databases
BRENDA	1.14.16.2. 672.
Gene expression databases
ArrayExpress	P90986.
Family and domain databases
InterPro	IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	879535.

Entry information

Entry name

TY3H_CAEEL

Accession

Primary (citable) accession number: P90986
Secondary accession number(s): Q5R3Y3, Q5R3Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 10, 2005
Last modified:	January 19, 2010
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project