Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P90980 (KPC2_CAEEL)

Last modified November 3, 2009. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Protein kinase C-like 2
      Short name=PKC2
    EC=2.7.11.13
Gene names
Name: pkc-2
Synonyms: kin-11
ORF Names: E01H11.1
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Hide | Top

Protein attributes

Sequence length682 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Function

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Hide | Top

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform b (identifier: P90980-1)

Also known as: PKC2B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform a (identifier: P90980-2)

Also known as: PKC2A;

The sequence of this isoform differs from the canonical sequence as follows:
     632-682: TDRSTENFDH...NPFYVKDVEP → SADDTSNFDS...FVNPEYVQEC
Isoform c (identifier: P90980-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MSLSTNSSVKEDE → MSLRPKQKSS...RPLLKRLHEQ
     682-682: P → PHIFYVLFIF...FVNPEYVQEC
Note: No experimental confirmation available.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 682682Protein kinase C-like 2
PRO_0000055736

Regions

Domain160 – 263104C2
Domain348 – 609262Protein kinase
Domain610 – 68172AGC-kinase C-terminal
Zinc finger38 – 8851Phorbol-ester/DAG-type 1
Zinc finger103 – 15351Phorbol-ester/DAG-type 2
Nucleotide binding354 – 3629ATP By similarity

Sites

Active site4721Proton acceptor By similarity
Binding site3771ATP By similarity

Natural variations

Alternative sequence1 – 1313MSLST…VKEDE → MSLRPKQKSSSTSVFLKSHK FSLTSHILRKAKKRETMDSA ERRRSETDIGGGPRNSADAR PSLDLSSDPMLESNLALRKL SEAFAAAALISPVKNNSTGG GGNQQLCIRKSISNPSSLKV YAHHEAPGIIHFPRFEFSHR LSIRPLLKRLHEQ in isoform c.
VSP_009369
Alternative sequence632 – 68251TDRST…KDVEP → SADDTSNFDSEFTHEVPKLT PIDRLFLMNLDQTEFEGFSF VNPEYVQEC in isoform a.
VSP_009370
Alternative sequence6821P → PHIFYVLFIFLILRPFLLLP YLSHLQLTLSAIHSPLLLLS ASVFFKFLFKNNFSQSPYNK IKVLLKSADDTSNFDSEFTH EVPKLTPIDRLFLMNLDQTE FEGFSFVNPEYVQEC in isoform c.
VSP_009371

Experimental info

Sequence conflict2001C → W in AAB40868. Ref.1
Sequence conflict2001C → W in AAB40869. Ref.1

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Isoform b (PKC2B) [UniParc].

Last modified February 16, 2004. Version 2.
Checksum: E41C5C12DA38ED3C

FASTA68278,000
        10         20         30         40         50         60 
MSLSTNSSVK EDEAQRIEGK AFVRRGALRQ KNVHEIKSHK FIARFFKQPT FCSHCKDFLW 

        70         80         90        100        110        120 
GITKQGFQCQ VCTLVVHKRC HEFVNFACPG ADKGVDTDDP RQQHKWKVQT YSSPTFCDHC 

       130        140        150        160        170        180 
GSLLYGILHQ GMKCQSCDTN VHHRCVKNVP NMCGTDNTEK RGRLRIEAHI ENDQLTIKIL 

       190        200        210        220        230        240 
EAKNLIPMDP NGLSDPYVKC KLIPEDSGCK SKQKTKTLRA TLNPQWNETF TYKLLPGDKD 

       250        260        270        280        290        300 
RRLSIEVWDW DRTSRNDFMG SLSFGISELM KEAASGWYKL LSAEEGEFYN INITPEYDED 

       310        320        330        340        350        360 
MEKVRKKMNE NFITRDNSSS KPKDPAAPRA STLPLGSSNH NVIKASDFNF LTVLGKGSFG 

       370        380        390        400        410        420 
KVLLGEQKTT KELFAIKVLK KDVIIQDDDV ECTMTEKRVL ALPEKPSFLV ALHSCFQTMD 

       430        440        450        460        470        480 
RLYFVMEFVN GGDLMYQIQQ VGKFKEPVAV FYAAEIAVGL FFLHSKGIIY RDLKLDNVML 

       490        500        510        520        530        540 
ERDGHIKITD FGMCKENIFG DATTKTFCGT PDYIAPEIIL YQPYGKSVDW WAYGVLLFEM 

       550        560        570        580        590        600 
LAGQPPFDGE DEDELFTAIT EHNVSYPKSL SKEAVSLCKA LLIKNPSKRL GCTGDDESAS 

       610        620        630        640        650        660 
RDIKEHPFFR RIDWFKIETR QIQPPFKPKL KTDRSTENFD HSFLKLPTKM TPPDWEVLEN 

       670        680 
LKGDEFSNFS FVNPFYVKDV EP

« Hide

Isoform a (PKC2A).

Checksum: 6CD177A781ED16C8
Show »

FASTA68077,695
Isoform c.

Checksum: 08B90BDED607DBF5
Show »

FASTA936106,866

Hide | Top

References

« Hide 'large scale' references
[1]"Structure and expression of the Caenorhabditis elegans protein kinase C2 gene. Origins and regulated expression of a family of Ca2+-activated protein kinase C isoforms."
Islas-Trejo A., Land M., Tcherepanova I., Freedman J.H., Rubin C.S.
J. Biol. Chem. 272:6629-6640(1997) [PubMed: 9045693] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

U82935 mRNA. Translation: AAB40868.1.
U82936 mRNA. Translation: AAB40869.1.
U29376 Genomic DNA. Translation: AAM51503.1.
U29376 Genomic DNA. Translation: AAM51504.1.
U29376 Genomic DNA. Translation: AAA68709.2.
PIRT15902.
RefSeqNP_001024516.1.
NP_001024517.1.
NP_001024518.1.
UniGeneCel.667

3D structure databases

HSSPHSSP built from PDB template 1DSY based on UniProtKB P05696.
ModBaseSearch...

Protein-protein interaction databases

IntActP90980. 7 interactions.

Proteomic databases

PRIDEP90980.

Genome annotation databases

EnsemblE01H11.1a; E01H11.1a; E01H11.1; Caenorhabditis elegans. [Genome view]
E01H11.1b; E01H11.1b; E01H11.1; Caenorhabditis elegans. [Genome view]
E01H11.1c; E01H11.1c; E01H11.1; Caenorhabditis elegans. [Genome view]
GeneID181166.
KEGGcel:E01H11.1.
UCSCE01H11.1d. c. elegans.

Organism-specific databases

CTD181166.
WormBaseWBGene00004033. pkc-2.
WormPepE01H11.1a. CE30927. [WorfDB]
E01H11.1b. CE30928. [WorfDB]
E01H11.1c. CE30929. [WorfDB]

Phylogenomic databases

OMAEEGEFYN.

Enzyme and pathway databases

BRENDA2.7.11.13. 672.

Gene expression databases

ArrayExpressP90980.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000008. C2_Ca-dep.
IPR018029. C2_membr_targeting.
IPR020477. C2_region.
IPR020454. DAG/PE_bd.
IPR015745. PKC.
IPR017892. Pkinase_C.
IPR002219. Prot_Kinase_C-like_PE/DAG_bd.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PANTHERPTHR22985:SF86. PKC. 1 hit.
PfamPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFPIRSF000550. PKC_alpha. 1 hit.
PRINTSPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio912688.

Hide | Top

Entry information

Entry nameKPC2_CAEEL
AccessionPrimary (citable) accession number: P90980
Secondary accession number(s): P90981 expand/collapse secondary AC list , Q19024, Q8MQ87, Q8MQ88
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: November 3, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

Hide | Top

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents