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Protein

Protein kinase C-like 2

Gene

pkc-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei377 – 3771ATPPROSITE-ProRule annotation
Active sitei472 – 4721Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 8851Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri103 – 15351Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi354 – 3629ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-dependent protein kinase C activity Source: WormBase
  • protein kinase C activity Source: WormBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • peptidyl-serine phosphorylation Source: WormBase
  • peptidyl-threonine phosphorylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-111933. Calmodulin induced events.
R-CEL-114516. Disinhibition of SNARE formation.
R-CEL-1169091. Activation of NF-kappaB in B cells.
R-CEL-2179392. EGFR Transactivation by Gastrin.
R-CEL-3000170. Syndecan interactions.
R-CEL-399997. Acetylcholine regulates insulin secretion.
R-CEL-4419969. Depolymerisation of the Nuclear Lamina.
R-CEL-450520. HuR (ELAVL1) binds and stabilizes mRNA.
R-CEL-5218921. VEGFR2 mediated cell proliferation.
R-CEL-76005. Response to elevated platelet cytosolic Ca2+.
SignaLinkiP90980.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C-like 2 (EC:2.7.11.13)
Short name:
PKC2
Gene namesi
Name:pkc-2
Synonyms:kin-11
ORF Names:E01H11.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiE01H11.1a; CE30927; WBGene00004033; pkc-2.
E01H11.1b; CE30928; WBGene00004033; pkc-2.
E01H11.1c; CE30929; WBGene00004033; pkc-2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: WormBase
  • membrane Source: WormBase
  • neuronal cell body Source: WormBase
  • neuron projection Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 682682Protein kinase C-like 2PRO_0000055736Add
BLAST

Proteomic databases

EPDiP90980.
PaxDbiP90980.
PRIDEiP90980.

Expressioni

Gene expression databases

ExpressionAtlasiP90980. baseline.

Interactioni

Protein-protein interaction databases

BioGridi46082. 7 interactions.
DIPiDIP-24386N.
MINTiMINT-1050273.
STRINGi6239.E01H11.1c.

Structurei

3D structure databases

ProteinModelPortaliP90980.
SMRiP90980. Positions 39-678.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini160 – 263104C2PROSITE-ProRule annotationAdd
BLAST
Domaini348 – 609262Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini610 – 68172AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 8851Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri103 – 15351Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233022.
InParanoidiP90980.
OMAiQSKKDMI.
OrthoDBiEOG77M8QM.
PhylomeDBiP90980.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000550. PKC_alpha. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b (identifier: P90980-1) [UniParc]FASTAAdd to basket

Also known as: PKC2B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLSTNSSVK EDEAQRIEGK AFVRRGALRQ KNVHEIKSHK FIARFFKQPT
60 70 80 90 100
FCSHCKDFLW GITKQGFQCQ VCTLVVHKRC HEFVNFACPG ADKGVDTDDP
110 120 130 140 150
RQQHKWKVQT YSSPTFCDHC GSLLYGILHQ GMKCQSCDTN VHHRCVKNVP
160 170 180 190 200
NMCGTDNTEK RGRLRIEAHI ENDQLTIKIL EAKNLIPMDP NGLSDPYVKC
210 220 230 240 250
KLIPEDSGCK SKQKTKTLRA TLNPQWNETF TYKLLPGDKD RRLSIEVWDW
260 270 280 290 300
DRTSRNDFMG SLSFGISELM KEAASGWYKL LSAEEGEFYN INITPEYDED
310 320 330 340 350
MEKVRKKMNE NFITRDNSSS KPKDPAAPRA STLPLGSSNH NVIKASDFNF
360 370 380 390 400
LTVLGKGSFG KVLLGEQKTT KELFAIKVLK KDVIIQDDDV ECTMTEKRVL
410 420 430 440 450
ALPEKPSFLV ALHSCFQTMD RLYFVMEFVN GGDLMYQIQQ VGKFKEPVAV
460 470 480 490 500
FYAAEIAVGL FFLHSKGIIY RDLKLDNVML ERDGHIKITD FGMCKENIFG
510 520 530 540 550
DATTKTFCGT PDYIAPEIIL YQPYGKSVDW WAYGVLLFEM LAGQPPFDGE
560 570 580 590 600
DEDELFTAIT EHNVSYPKSL SKEAVSLCKA LLIKNPSKRL GCTGDDESAS
610 620 630 640 650
RDIKEHPFFR RIDWFKIETR QIQPPFKPKL KTDRSTENFD HSFLKLPTKM
660 670 680
TPPDWEVLEN LKGDEFSNFS FVNPFYVKDV EP
Length:682
Mass (Da):78,000
Last modified:February 16, 2004 - v2
Checksum:iE41C5C12DA38ED3C
GO
Isoform a (identifier: P90980-2) [UniParc]FASTAAdd to basket

Also known as: PKC2A

The sequence of this isoform differs from the canonical sequence as follows:
     632-682: TDRSTENFDH...NPFYVKDVEP → SADDTSNFDS...FVNPEYVQEC

Show »
Length:680
Mass (Da):77,695
Checksum:i6CD177A781ED16C8
GO
Isoform c (identifier: P90980-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MSLSTNSSVKEDE → MSLRPKQKSS...RPLLKRLHEQ
     682-682: P → PHIFYVLFIF...FVNPEYVQEC

Note: No experimental confirmation available.
Show »
Length:936
Mass (Da):106,866
Checksum:i08B90BDED607DBF5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti200 – 2001C → W in AAB40868 (PubMed:9045693).Curated
Sequence conflicti200 – 2001C → W in AAB40869 (PubMed:9045693).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1313MSLST…VKEDE → MSLRPKQKSSSTSVFLKSHK FSLTSHILRKAKKRETMDSA ERRRSETDIGGGPRNSADAR PSLDLSSDPMLESNLALRKL SEAFAAAALISPVKNNSTGG GGNQQLCIRKSISNPSSLKV YAHHEAPGIIHFPRFEFSHR LSIRPLLKRLHEQ in isoform c. CuratedVSP_009369Add
BLAST
Alternative sequencei632 – 68251TDRST…KDVEP → SADDTSNFDSEFTHEVPKLT PIDRLFLMNLDQTEFEGFSF VNPEYVQEC in isoform a. 1 PublicationVSP_009370Add
BLAST
Alternative sequencei682 – 6821P → PHIFYVLFIFLILRPFLLLP YLSHLQLTLSAIHSPLLLLS ASVFFKFLFKNNFSQSPYNK IKVLLKSADDTSNFDSEFTH EVPKLTPIDRLFLMNLDQTE FEGFSFVNPEYVQEC in isoform c. CuratedVSP_009371

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82935 mRNA. Translation: AAB40868.1.
U82936 mRNA. Translation: AAB40869.1.
FO080629 Genomic DNA. Translation: CCD65287.1.
FO080629 Genomic DNA. Translation: CCD65288.1.
FO080629 Genomic DNA. Translation: CCD65289.1.
PIRiT15902.
RefSeqiNP_001024516.1. NM_001029345.3. [P90980-2]
NP_001024517.1. NM_001029346.2. [P90980-1]
NP_001024518.1. NM_001029347.1. [P90980-3]
UniGeneiCel.667.

Genome annotation databases

EnsemblMetazoaiE01H11.1b; E01H11.1b; WBGene00004033. [P90980-1]
GeneIDi181166.
UCSCiE01H11.1d. c. elegans. [P90980-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82935 mRNA. Translation: AAB40868.1.
U82936 mRNA. Translation: AAB40869.1.
FO080629 Genomic DNA. Translation: CCD65287.1.
FO080629 Genomic DNA. Translation: CCD65288.1.
FO080629 Genomic DNA. Translation: CCD65289.1.
PIRiT15902.
RefSeqiNP_001024516.1. NM_001029345.3. [P90980-2]
NP_001024517.1. NM_001029346.2. [P90980-1]
NP_001024518.1. NM_001029347.1. [P90980-3]
UniGeneiCel.667.

3D structure databases

ProteinModelPortaliP90980.
SMRiP90980. Positions 39-678.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi46082. 7 interactions.
DIPiDIP-24386N.
MINTiMINT-1050273.
STRINGi6239.E01H11.1c.

Proteomic databases

EPDiP90980.
PaxDbiP90980.
PRIDEiP90980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiE01H11.1b; E01H11.1b; WBGene00004033. [P90980-1]
GeneIDi181166.
UCSCiE01H11.1d. c. elegans. [P90980-1]

Organism-specific databases

CTDi181166.
WormBaseiE01H11.1a; CE30927; WBGene00004033; pkc-2.
E01H11.1b; CE30928; WBGene00004033; pkc-2.
E01H11.1c; CE30929; WBGene00004033; pkc-2.

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233022.
InParanoidiP90980.
OMAiQSKKDMI.
OrthoDBiEOG77M8QM.
PhylomeDBiP90980.

Enzyme and pathway databases

ReactomeiR-CEL-111933. Calmodulin induced events.
R-CEL-114516. Disinhibition of SNARE formation.
R-CEL-1169091. Activation of NF-kappaB in B cells.
R-CEL-2179392. EGFR Transactivation by Gastrin.
R-CEL-3000170. Syndecan interactions.
R-CEL-399997. Acetylcholine regulates insulin secretion.
R-CEL-4419969. Depolymerisation of the Nuclear Lamina.
R-CEL-450520. HuR (ELAVL1) binds and stabilizes mRNA.
R-CEL-5218921. VEGFR2 mediated cell proliferation.
R-CEL-76005. Response to elevated platelet cytosolic Ca2+.
SignaLinkiP90980.

Miscellaneous databases

PROiP90980.

Gene expression databases

ExpressionAtlasiP90980. baseline.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000550. PKC_alpha. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of the Caenorhabditis elegans protein kinase C2 gene. Origins and regulated expression of a family of Ca2+-activated protein kinase C isoforms."
    Islas-Trejo A., Land M., Tcherepanova I., Freedman J.H., Rubin C.S.
    J. Biol. Chem. 272:6629-6640(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiKPC2_CAEEL
AccessioniPrimary (citable) accession number: P90980
Secondary accession number(s): P90981
, Q19024, Q8MQ87, Q8MQ88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: June 8, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.