Reviewed, UniProtKB/Swiss-Prot P90925 (PH4H_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable phenylalanine-4-hydroxylase 1
      Short name=PAH
    EC=1.14.16.1
Alternative name(s):
    Phe-4-monooxygenase
    Biogenic amine synthesis protein 2

Gene names

Name:	pah-1
Synonyms:	bas-2
ORF Names:	K08F8.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-phenylalanine + tetrahydrobiopterin + O2 = L-tyrosine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 1/6.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Phenylalanine catabolism
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine 4-monooxygenase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9XX47	1	EBI-318020,EBI-330289
bath-42	P34371	1	EBI-318020,EBI-315422
Y116A8C.26	Q9U2U6	1	EBI-318020,EBI-329992

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Probable phenylalanine-4-hydroxylase 1		PRO_0000205551
Regions
	Domain	30 – 107	78	ACT
Sites
	Metal binding	285	1	Iron By similarity
	Metal binding	290	1	Iron By similarity
	Metal binding	330	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P90925-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 68365836DFEC8D4F	FASTA	457	52,129
	10 20 30 40 50 60 MPPAGQDDLD FLKYAMESYV ADVNADIGKT TIVFTLREKA GALAETLKLF QAHDVNLSHI 70 80 90 100 110 120 ESRPSKTHEG CYEVLVEFAE AEDHRKIEGV IEHFQQKAEK KVLVQDWNTK NKQNKDSVPW 130 140 150 160 170 180 FPQKINDIDQ FANRILSYGA ELDADHPGFK DMTYRERRKF FADIAFNFKH GDKIPTITYT 190 200 210 220 230 240 DEEIATWRTV YNELTVMYPK NACQEFNYIF PLLQQNCGFG PDRIPQLQDV SDFLKDCTGY 250 260 270 280 290 300 TIRPVAGLLS SRDFLAGLAF RVFHSTQYIR HHSAPKYTPE PDICHELLGH VPLFADVEFA 310 320 330 340 350 360 QFSQEIGLAS LGAPDDVIEK LATLYWFTIE FGICQQDGEK KAYGAGLLSS FGELQYALSD 370 380 390 400 410 420 KPEVVDFDPA VCCVTKYPIT EYQPKYFLAE SFASAKNKLK SWAATINRPF QIRYNAYTQR 430 440 450 VEILDKVAAL QRLARDIRSD ISTLEEALGK VNNLKMK

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z66497 Genomic DNA. Translation: CAA91286.1.
PIR	T23494.
RefSeq	NP_495863.1.
UniGene	Cel.19578
3D structure databases
HSSP	HSSP built from PDB template 1PHZ based on UniProtKB P04176.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:25264N.
IntAct	P90925. 5 interactions.
Genome annotation databases
Ensembl	K08F8.4. Caenorhabditis elegans. [Contig view]
GeneID	174401.
KEGG	cel:K08F8.4.
NMPDR	fig|6239.3.peg.6723.
Organism-specific databases
WormBase	WBGene00000240. pah-1.
WormPep	K08F8.4. CE21050. [WorfDB]
Phylogenomic databases
OMA	P90925. QETSYIE.
Enzyme and pathway databases
BRENDA	1.14.16.1. 672.
Gene expression databases
ArrayExpress	P90925.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005961. Phe-4-hydroxylase_tetra. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01268. Phe4hydrox_tetr. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	883882.

Entry information

Entry name

PH4H_CAEEL

Accession

Primary (citable) accession number: P90925

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project