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P90866

- CDK8_CAEEL

UniProt

P90866 - CDK8_CAEEL

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Protein

Cyclin-dependent kinase 8

Gene

cdk-8

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601ATPPROSITE-ProRule annotation
Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 409ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. G1/S transition of mitotic cell cycle Source: UniProtKB
  2. protein phosphorylation Source: UniProtKB
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_183534. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
REACT_269089. NOTCH1 Intracellular Domain Regulates Transcription.
REACT_270292. Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants.
REACT_270666. Constitutive Signaling by NOTCH1 PEST Domain Mutants.
SignaLinkiP90866.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 8 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit cdk-8
Mediator of RNA polymerase II transcription subunit cdk-8
Gene namesi
Name:cdk-8
ORF Names:F39H11.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiF39H11.3; CE32409; WBGene00000409; cdk-8.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 588588Cyclin-dependent kinase 8PRO_0000312931Add
BLAST

Proteomic databases

PaxDbiP90866.
PRIDEiP90866.

Interactioni

Subunit structurei

Component of the Mediator complex.By similarity

Protein-protein interaction databases

BioGridi38110. 2 interactions.
STRINGi6239.F39H11.3.

Structurei

3D structure databases

ProteinModelPortaliP90866.
SMRiP90866. Positions 5-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 348323Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi374 – 585212Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000064012.
InParanoidiP90866.
KOiK02208.
OMAiFPNSLHR.
OrthoDBiEOG76739K.
PhylomeDBiP90866.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P90866-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLMIDENFK KQLAQRRERV EDLFYFENSK EIGRGTYGLV YKAVPKKQNG
60 70 80 90 100
QFPNKEYALK MIEGQGFSMS ACREIALFRE LRHPNLICLQ RVFLTNEKKV
110 120 130 140 150
WLLLDYAEHD LWHVIKHHRT AKSKKVPIMV PRNMVKNILF QILSGMHYLH
160 170 180 190 200
SNWVLHRDLK PANILLMGDG PPDMRGRVKI ADLGFSRIYA NPLKPMAELD
210 220 230 240 250
PVVVTFWYRA PELLLGAKHY TKAIDVWAIG CIFAELLTAE PLFFCKEEDI
260 270 280 290 300
KAQNPYHYDQ VKRIFHLLGY PSDADWPDMK KMPDHQRLLS DARNEGTPIQ
310 320 330 340 350
TFPNSLHRYF DKWKINSQSS PYRLLVKLLT VDPTKRVSCE EAMNDIYFRK
360 370 380 390 400
MERPPRETDD VFNKYPIPYA KKEQQMTVAP DQAQQQHQQQ QVQMQQQPQM
410 420 430 440 450
GQQQMMGQPQ MVQPQMGQPP MGGAHPGVVA PDGHPHQMMQ QQQHPQQHHM
460 470 480 490 500
QYQGMHDPMQ GGMDEGPQAK MMRMGNVPVG RYAPMPPPYG APQDYHPQQG
510 520 530 540 550
PPMVQMMQQP GPSGYYPQRP GQPTGAVPGP GPQGYMNPQM GMQMGMRAPG
560 570 580
VPPQGYMPGR GMAPPQMGQQ QPGPNQQQQQ QWQQQYHR
Length:588
Mass (Da):67,785
Last modified:March 1, 2003 - v4
Checksum:i7409832B107F6563
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81079 Genomic DNA. Translation: CAB03083.2.
PIRiT22001.
RefSeqiNP_492357.2. NM_059956.6.
UniGeneiCel.22900.

Genome annotation databases

EnsemblMetazoaiF39H11.3; F39H11.3; WBGene00000409.
GeneIDi172677.
KEGGicel:CELE_F39H11.3.
UCSCiF39H11.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81079 Genomic DNA. Translation: CAB03083.2 .
PIRi T22001.
RefSeqi NP_492357.2. NM_059956.6.
UniGenei Cel.22900.

3D structure databases

ProteinModelPortali P90866.
SMRi P90866. Positions 5-373.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 38110. 2 interactions.
STRINGi 6239.F39H11.3.

Proteomic databases

PaxDbi P90866.
PRIDEi P90866.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai F39H11.3 ; F39H11.3 ; WBGene00000409 .
GeneIDi 172677.
KEGGi cel:CELE_F39H11.3.
UCSCi F39H11.3. c. elegans.

Organism-specific databases

CTDi 172677.
WormBasei F39H11.3 ; CE32409 ; WBGene00000409 ; cdk-8.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000064012.
InParanoidi P90866.
KOi K02208.
OMAi FPNSLHR.
OrthoDBi EOG76739K.
PhylomeDBi P90866.

Enzyme and pathway databases

Reactomei REACT_183534. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
REACT_269089. NOTCH1 Intracellular Domain Regulates Transcription.
REACT_270292. Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants.
REACT_270666. Constitutive Signaling by NOTCH1 PEST Domain Mutants.
SignaLinki P90866.

Miscellaneous databases

NextBioi 876533.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiCDK8_CAEEL
AccessioniPrimary (citable) accession number: P90866
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 119 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3