Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P90866 (CDK8_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 8

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit cdk-8
Mediator of RNA polymerase II transcription subunit cdk-8
Gene names
Name:cdk-8
ORF Names:F39H11.3
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Component of the Mediator complex By similarity.

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 588588Cyclin-dependent kinase 8
PRO_0000312931

Regions

Domain26 – 348323Protein kinase
Nucleotide binding32 – 409ATP By similarity
Compositional bias374 – 585212Gln-rich

Sites

Active site1581Proton acceptor By similarity
Binding site601ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P90866 [UniParc].

Last modified March 1, 2003. Version 4.
Checksum: 7409832B107F6563

FASTA58867,785
        10         20         30         40         50         60 
MTLMIDENFK KQLAQRRERV EDLFYFENSK EIGRGTYGLV YKAVPKKQNG QFPNKEYALK 

        70         80         90        100        110        120 
MIEGQGFSMS ACREIALFRE LRHPNLICLQ RVFLTNEKKV WLLLDYAEHD LWHVIKHHRT 

       130        140        150        160        170        180 
AKSKKVPIMV PRNMVKNILF QILSGMHYLH SNWVLHRDLK PANILLMGDG PPDMRGRVKI 

       190        200        210        220        230        240 
ADLGFSRIYA NPLKPMAELD PVVVTFWYRA PELLLGAKHY TKAIDVWAIG CIFAELLTAE 

       250        260        270        280        290        300 
PLFFCKEEDI KAQNPYHYDQ VKRIFHLLGY PSDADWPDMK KMPDHQRLLS DARNEGTPIQ 

       310        320        330        340        350        360 
TFPNSLHRYF DKWKINSQSS PYRLLVKLLT VDPTKRVSCE EAMNDIYFRK MERPPRETDD 

       370        380        390        400        410        420 
VFNKYPIPYA KKEQQMTVAP DQAQQQHQQQ QVQMQQQPQM GQQQMMGQPQ MVQPQMGQPP 

       430        440        450        460        470        480 
MGGAHPGVVA PDGHPHQMMQ QQQHPQQHHM QYQGMHDPMQ GGMDEGPQAK MMRMGNVPVG 

       490        500        510        520        530        540 
RYAPMPPPYG APQDYHPQQG PPMVQMMQQP GPSGYYPQRP GQPTGAVPGP GPQGYMNPQM 

       550        560        570        580 
GMQMGMRAPG VPPQGYMPGR GMAPPQMGQQ QPGPNQQQQQ QWQQQYHR 

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z81079 Genomic DNA. Translation: CAB03083.2.
PIRT22001.
RefSeqNP_492357.2. NM_059956.6.
UniGeneCel.22900.

3D structure databases

ProteinModelPortalP90866.
SMRP90866. Positions 5-373.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid38110. 2 interactions.
STRING6239.F39H11.3.

Proteomic databases

PaxDbP90866.
PRIDEP90866.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF39H11.3; F39H11.3; WBGene00000409.
GeneID172677.
KEGGcel:CELE_F39H11.3.
UCSCF39H11.3. c. elegans.

Organism-specific databases

CTD172677.
WormBaseF39H11.3; CE32409; WBGene00000409; cdk-8.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00530000064012.
InParanoidP90866.
KOK02208.
OMAFPNSLHR.
OrthoDBEOG76739K.
PhylomeDBP90866.

Enzyme and pathway databases

SignaLinkP90866.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio876533.

Entry information

Entry nameCDK8_CAEEL
AccessionPrimary (citable) accession number: P90866
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 116 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase