P90866 (CDK8_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 8
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit cdk-8
Mediator of RNA polymerase II transcription subunit cdk-8

Gene names

Name:	cdk-8
ORF Names:	F39H11.3

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the Mediator complex By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G1/S transition of mitotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB body morphogenesis Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW reproduction Inferred from mutant phenotype. Source: WormBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Cyclin-dependent kinase 8		PRO_0000312931
Regions
	Domain	26 – 348	323	Protein kinase
	Nucleotide binding	32 – 40	9	ATP By similarity
	Compositional bias	374 – 585	212	Gln-rich
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	60	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P90866 [UniParc]. Last modified March 1, 2003. Version 4. Checksum: 7409832B107F6563	FASTA	588	67,785
	10 20 30 40 50 60 MTLMIDENFK KQLAQRRERV EDLFYFENSK EIGRGTYGLV YKAVPKKQNG QFPNKEYALK 70 80 90 100 110 120 MIEGQGFSMS ACREIALFRE LRHPNLICLQ RVFLTNEKKV WLLLDYAEHD LWHVIKHHRT 130 140 150 160 170 180 AKSKKVPIMV PRNMVKNILF QILSGMHYLH SNWVLHRDLK PANILLMGDG PPDMRGRVKI 190 200 210 220 230 240 ADLGFSRIYA NPLKPMAELD PVVVTFWYRA PELLLGAKHY TKAIDVWAIG CIFAELLTAE 250 260 270 280 290 300 PLFFCKEEDI KAQNPYHYDQ VKRIFHLLGY PSDADWPDMK KMPDHQRLLS DARNEGTPIQ 310 320 330 340 350 360 TFPNSLHRYF DKWKINSQSS PYRLLVKLLT VDPTKRVSCE EAMNDIYFRK MERPPRETDD 370 380 390 400 410 420 VFNKYPIPYA KKEQQMTVAP DQAQQQHQQQ QVQMQQQPQM GQQQMMGQPQ MVQPQMGQPP 430 440 450 460 470 480 MGGAHPGVVA PDGHPHQMMQ QQQHPQQHHM QYQGMHDPMQ GGMDEGPQAK MMRMGNVPVG 490 500 510 520 530 540 RYAPMPPPYG APQDYHPQQG PPMVQMMQQP GPSGYYPQRP GQPTGAVPGP GPQGYMNPQM 550 560 570 580 GMQMGMRAPG VPPQGYMPGR GMAPPQMGQQ QPGPNQQQQQ QWQQQYHR

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z81079 Genomic DNA. Translation: CAB03083.2.
PIR	T22001.
RefSeq	NP_492357.2. NM_059956.5.
UniGene	Cel.22900.
3D structure databases
HSSP	HSSP built from PDB template 1BI8 based on UniProtKB Q00534.
ProteinModelPortal	P90866.
SMR	P90866. Positions 22-354.
ModBase	Search...
Protein-protein interaction databases
STRING	P90866.
Proteomic databases
PRIDE	P90866.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F39H11.3; F39H11.3; F39H11.3.
GeneID	172677.
KEGG	cel:F39H11.3.
UCSC	F39H11.3. c. elegans.
Organism-specific databases
CTD	172677.
WormBase	F39H11.3; CE32409; WBGene00000409; cdk-8.
Phylogenomic databases
eggNOG	meNOG04705.
GeneTree	EMGT00050000001395.
HOGENOM	HBG755340.
InParanoid	P90866.
OMA	SDARNEG.
PhylomeDB	P90866.
Gene expression databases
ArrayExpress	P90866.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K02208.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	876533.

Entry information

Entry name

CDK8_CAEEL

Accession

Primary (citable) accession number: P90866

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2003
Last modified:	November 16, 2011
This is version 96 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project