P90795 (GPDM_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glycerol-3-phosphate dehydrogenase, mitochondrial
Short name=GPD-M
Short name=GPDH-M
EC=1.1.5.3

Gene names

ORF Names:

T25G3.4

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	722 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.
Cofactor	FAD By similarity.
Enzyme regulation	Calcium-binding enhances the activity of the enzyme By similarity.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	Calcium FAD Flavoprotein Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycerol catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Mitochondrion
	Chain	44 – 722	679	Probable glycerol-3-phosphate dehydrogenase, mitochondrial		PRO_0000010432
Regions
	Domain	624 – 659	36	EF-hand 1
	Domain	660 – 695	36	EF-hand 2
	Nucleotide binding	76 – 104	29	FAD Potential
	Calcium binding	673 – 684	12	Potential

Sequences

	Sequence		Length	Mass (Da)
	P90795 [UniParc]. Last modified November 1, 1998. Version 2. Checksum: 9E8BF74246480E30	FASTA	722	80,807
	10 20 30 40 50 60 MSWVRFTKTG VAVVATSAAA VLALDMTNER RFQRQVKDHF RTVHADRLAE LNKRAPSALP 70 80 90 100 110 120 TRKDILTNLS KGEEFDVLII GGGATGAGVA LDAQTRGLKT ALVELDDFSS GTSSRSTKLI 130 140 150 160 170 180 HGGVRYLQAA IMKLDLEQYR MVKEALFERH NLLEIAPHLS SPLPIMLPIY KLWQVPYYWS 190 200 210 220 230 240 GIKAYDFVSG KRVLKNSFFI NKSQALERFP MLRNESLKGA LIYYDGQHND ARMNLAIILT 250 260 270 280 290 300 AIRHGAACAN HVRVEKLNKD ETGKVIGAHV RDMVTGGEWD IKAKAVINAT GPFTDSIRLM 310 320 330 340 350 360 GDPETARPIC APSSGVHITL PGYYSPSNTG LLDPDTSDGR VIFFLPWERM TIAGTTDAPS 370 380 390 400 410 420 DVTLSPQPTD HDIEFILQEI RGYLSKDVSV RRGDVMSAWS GLRPLVRDPN KKDTKSLARN 430 440 450 460 470 480 HIIEVGKSGL ITIAGGKWTT YRHMAEETVD RVVEVHGLKT ENGCVTPGLL LEGAHDWNSL 490 500 510 520 530 540 QYIHLVQDYG MEVDVAQHLS NTYGDRAFVV ARMCKMTGKR WPIVGQRLHP EFPYLDAEVR 550 560 570 580 590 600 YAVREYACTA IDVIARRMRL AFLNTYAAHE VLPDVVRVMG QELGWSSAEQ RAQLEKARTF 610 620 630 640 650 660 IDMEMGQNAK QTAVSNVALN LTKEEMQRAK ERFQQLDKDR KGHITVNDLR KHFREHNQKI 670 680 690 700 710 720 DERVLHELLN EVDLNKNGEI EIAEFFQLYS GLKGGQLTGN RLVGYLDEIH GTPSVNRACG GI

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72516, Z73906 Genomic DNA. Translation: CAA96690.1.
PIR	T20362.
RefSeq	NP_492115.1. NM_059714.4.
3D structure databases
ProteinModelPortal	P90795.
SMR	P90795. Positions 73-590, 621-690.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.T25G3.4.
Proteomic databases
PaxDb	P90795.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T25G3.4.1; T25G3.4.1; T25G3.4. T25G3.4.2; T25G3.4.2; T25G3.4.
GeneID	172509.
KEGG	cel:CELE_T25G3.4.
UCSC	T25G3.4. c. elegans.
Organism-specific databases
CTD	172509.
WormBase	T25G3.4; CE14180; WBGene00012031.
Phylogenomic databases
eggNOG	COG0578.
GeneTree	ENSGT00390000001718.
HOGENOM	HOG000004813.
InParanoid	P90795.
KO	K00111.
OMA	DSIRKMD.
Enzyme and pathway databases
UniPathway	UPA00618; UER00673.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR006076. FAD-dep_OxRdtase. IPR000447. G3P_DH_FAD-dep. [Graphical view]
Pfam	PF01266. DAO. 1 hit. PF13499. EF_hand_5. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	875825.

Entry information

Entry name

GPDM_CAEEL

Accession

Primary (citable) accession number: P90795
Secondary accession number(s): Q22793

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project