Reviewed, UniProtKB/Swiss-Prot P90795 (GPDM_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable glycerol-3-phosphate dehydrogenase, mitochondrial
      Short name=GPDH-M
      Short name=GPD-M
    EC=1.1.5.3

Gene names

ORF Names:

T25G3.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	722 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.
Cofactor	FAD By similarity.
Enzyme regulation	Calcium-binding enhance the activity of the enzyme.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	Calcium FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Mitochondrion
	Chain	44 – 722	679	Probable glycerol-3-phosphate dehydrogenase, mitochondrial		PRO_0000010432
Regions
	Domain	624 – 659	36	EF-hand 1
	Domain	660 – 695	36	EF-hand 2
	Nucleotide binding	76 – 104	29	FAD Potential
	Calcium binding	673 – 684	12	Potential

Sequences

	Sequence		Length	Mass (Da)
	P90795-1 [UniParc]. Last modified November 1, 1998. Version 2. Checksum: 9E8BF74246480E30	FASTA	722	80,807
	10 20 30 40 50 60 MSWVRFTKTG VAVVATSAAA VLALDMTNER RFQRQVKDHF RTVHADRLAE LNKRAPSALP 70 80 90 100 110 120 TRKDILTNLS KGEEFDVLII GGGATGAGVA LDAQTRGLKT ALVELDDFSS GTSSRSTKLI 130 140 150 160 170 180 HGGVRYLQAA IMKLDLEQYR MVKEALFERH NLLEIAPHLS SPLPIMLPIY KLWQVPYYWS 190 200 210 220 230 240 GIKAYDFVSG KRVLKNSFFI NKSQALERFP MLRNESLKGA LIYYDGQHND ARMNLAIILT 250 260 270 280 290 300 AIRHGAACAN HVRVEKLNKD ETGKVIGAHV RDMVTGGEWD IKAKAVINAT GPFTDSIRLM 310 320 330 340 350 360 GDPETARPIC APSSGVHITL PGYYSPSNTG LLDPDTSDGR VIFFLPWERM TIAGTTDAPS 370 380 390 400 410 420 DVTLSPQPTD HDIEFILQEI RGYLSKDVSV RRGDVMSAWS GLRPLVRDPN KKDTKSLARN 430 440 450 460 470 480 HIIEVGKSGL ITIAGGKWTT YRHMAEETVD RVVEVHGLKT ENGCVTPGLL LEGAHDWNSL 490 500 510 520 530 540 QYIHLVQDYG MEVDVAQHLS NTYGDRAFVV ARMCKMTGKR WPIVGQRLHP EFPYLDAEVR 550 560 570 580 590 600 YAVREYACTA IDVIARRMRL AFLNTYAAHE VLPDVVRVMG QELGWSSAEQ RAQLEKARTF 610 620 630 640 650 660 IDMEMGQNAK QTAVSNVALN LTKEEMQRAK ERFQQLDKDR KGHITVNDLR KHFREHNQKI 670 680 690 700 710 720 DERVLHELLN EVDLNKNGEI EIAEFFQLYS GLKGGQLTGN RLVGYLDEIH GTPSVNRACG GI

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z73906, Z72516 Genomic DNA. Translation: CAA98123.1. Z72516, Z73906 Genomic DNA. Translation: CAA96690.1.
PIR	T20362.
RefSeq	NP_492115.1.
UniGene	Cel.7665
3D structure databases
HSSP	HSSP built from PDB template 1AK8 based on UniProtKB P02593.
ModBase	Search...
Genome annotation databases
Ensembl	T25G3.4. Caenorhabditis elegans. [Contig view]
GeneID	172509.
KEGG	cel:T25G3.4.
NMPDR	fig|6239.3.peg.1942.
Organism-specific databases
WormBase	WBGene00012031. T25G3.4.
WormPep	T25G3.4. CE14180. [WorfDB]
Phylogenomic databases
OMA	P90795. DSIRKMD.
Enzyme and pathway databases
BRENDA	1.1.99.5. 672.
Gene expression databases
ArrayExpress	P90795.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR018248. EF_hand. IPR018247. EF_HAND_1. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR000447. FAD-dep_Gly3P_DH. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF01266. DAO. 1 hit. PF00036. efhand. 2 hits. [Graphical view]
PRINTS	PR01001. FADG3PDH.
ProDom	PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	875825.

Entry information

Entry name

GPDM_CAEEL

Accession

Primary (citable) accession number: P90795
Secondary accession number(s): Q22793

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project