P90740 (FAN1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fanconi-associated nuclease 1 homolog
EC=3.1.-.-

Gene names

Name:	fan-1
ORF Names:	C01G5.8

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	865 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nuclease involved in DNA repair of DNA interstrand cross-links (ICL). Ref.2 Ref.3 Ref.4
Cofactor	Divalent metal cations. Mg2+ is the most probable By similarity.
Subcellular location	Nucleus By similarity.
Disruption phenotype	No visible phenotype in normal conditions. Strong increase of embryonic lethality following cisplatin, nitrogen mustard or mitomycin-C (MMC) treatment. Ref.2 Ref.3 Ref.4
Sequence similarities	Belongs to the FAN1 family. Contains 1 VRR-NUC domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from mutant phenotype Ref.2Ref.3. Source: UniProtKB
Cellular component	nucleus Inferred from direct assay Ref.3. Source: WormBase
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nuclease activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
smo-1	P55853	3	EBI-313626,EBI-313647

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 865	865	Fanconi-associated nuclease 1 homolog		PRO_0000398622
Regions
	Domain	744 – 857	114	VRR-NUC

Sequences

	Sequence		Length	Mass (Da)
	P90740 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 3F11059CEB769B50	FASTA	865	99,406
	10 20 30 40 50 60 MKKAKKEKKE KIIKPDGPIN FNRSIMAAFE KQSRGKICPL CETKFSLASY KSHMNTCNVA 70 80 90 100 110 120 DDDEEIEVIA TYTRDEAILM RAGPEIILGD ASFSDKSENP TKRRKTDERE VPSEDDIVPE 130 140 150 160 170 180 VPGPSGIVKN HEMPSESLDV TEISENIEKV IKKSPEWINH RRRSSRLLQN SQKDQADNAN 190 200 210 220 230 240 KEDPVKKETA TISEVLQAIE RFEQRVSGPE QTWPYYIKIT IKIMKRVIST EKFDGTFYAD 250 260 270 280 290 300 DFWLPSDIIT FYRFVELLSE GAKCLLVRLF IRKPAWYNLE KLEQKYPEIP NIKEAVSELA 310 320 330 340 350 360 KGHFIDDDSS MKTLDEALQI SDVVALKNVT KKFKLDGTKN RQELIQSLRK FAQSQQSIFG 370 380 390 400 410 420 GTGNVEKSIL KSLKQELGPC VRVRGGFVDL FKCLFTIYCP VTTNSANVID NPSTTNVYQD 430 440 450 460 470 480 LLYLMLSVAN GTVQFPAPNP CPIIASFYKN RNMLQDYMIS KSLEIAIVSQ MSNGNLDAAL 490 500 510 520 530 540 DLAIDAKEFI EQMSDDDKRY YESLEIHERK FTSIWVFTRC CGHASSILER QKKYGMAVEW 550 560 570 580 590 600 QKDLLITNKD IQSYCIDSRG IWWDRMLLNL DSHLKEKKEC AKMIQIALQD PSILEKELLM 610 620 630 640 650 660 IQDRALKLKE MPADFVTPIN IGNPEKKTIT ANVITKSLGD GRINRFMIRD HETDDDVECS 670 680 690 700 710 720 VEEVTRRHYL ENEGFSTGVH DEGSTWHTLF GLFFYDVIFA TDESVESTWL SELQDCPSDL 730 740 750 760 770 780 SNTLYSKRKE KFEDRFVWLE EAEQELIEEN IRKIWDLKHN ETNRECSWKQ FPMGAEDCVS 790 800 810 820 830 840 FFQCIPRPAL ILILRRLAEN YRNSRSGFPD LTLWNPETKR VAVVEVKGPG DRLSTKQRLW 850 860 LAIFADSGIR AEVCHVAAQN SRLLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Identification of KIAA1018/FAN1, a DNA repair nuclease recruited to DNA damage by monoubiquitinated FANCD2." MacKay C., Declais A.C., Lundin C., Agostinho A., Deans A.J., MacArtney T.J., Hofmann K., Gartner A., West S.C., Helleday T., Lilley D.M., Rouse J. Cell 142:65-76(2010) [PubMed: 20603015] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[3]	"Deficiency of FANCD2-associated nuclease KIAA1018/FAN1 sensitizes cells to interstrand crosslinking agents." Kratz K., Schopf B., Kaden S., Sendoel A., Eberhard R., Lademann C., Cannavo E., Sartori A.A., Hengartner M.O., Jiricny J. Cell 142:77-88(2010) [PubMed: 20603016] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[4]	"A genetic screen identifies FAN1, a Fanconi anemia-associated nuclease necessary for DNA interstrand crosslink repair." Smogorzewska A., Desetty R., Saito T.T., Schlabach M., Lach F.P., Sowa M.E., Clark A.B., Kunkel T.A., Harper J.W., Colaiacovo M.P., Elledge S.J. Mol. Cell 39:36-47(2010) [PubMed: 20603073] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080264 Genomic DNA. Translation: CCD62449.1.
PIR	T30998.
RefSeq	NP_500997.1. NM_068596.3.
UniGene	Cel.6011.
3D structure databases
ProteinModelPortal	P90740.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24423N.
IntAct	P90740. 3 interactions.
MINT	MINT-1069731.
STRING	P90740.
Proteomic databases
PRIDE	P90740.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C01G5.8; C01G5.8; C01G5.8.
GeneID	177415.
KEGG	cel:C01G5.8.
NMPDR	fig|6239.3.peg.13688.
UCSC	C01G5.8. c. elegans.
Organism-specific databases
CTD	177415.
WormBase	C01G5.8; CE07800; WBGene00015310; fan-1.
Phylogenomic databases
eggNOG	meNOG11142.
GeneTree	EMGT00050000013694.
HOGENOM	HBG383373.
InParanoid	P90740.
OMA	DAKVQGK.
PhylomeDB	P90740.
Gene expression databases
ArrayExpress	P90740.
Family and domain databases
InterPro	IPR014883. VRR_NUC. [Graphical view]
KO	K15363.
Pfam	PF08774. VRR_NUC. 1 hit. [Graphical view]
SMART	SM00990. VRR_NUC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	896716.

Entry information

Entry name

FAN1_CAEEL

Accession

Primary (citable) accession number: P90740

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project