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Protein

Myosin heavy chain kinase B

Gene

mhkB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Phosphorylates threonine in the C-terminal tail region of myosin II heavy chain. This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament.

Catalytic activityi

ATP + [myosin heavy-chain] = ADP + [myosin heavy-chain] phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi298 – 303ATPSequence analysis6

GO - Molecular functioni

  • ATP binding Source: dictyBase
  • myosin heavy chain kinase activity Source: dictyBase
  • myosin II binding Source: dictyBase
  • protein serine/threonine kinase activity Source: dictyBase

GO - Biological processi

  • mitotic cytokinesis Source: dictyBase
  • myosin II filament disassembly Source: dictyBase
  • peptidyl-threonine phosphorylation Source: dictyBase
  • protein autophosphorylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.7. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin heavy chain kinase B (EC:2.7.11.7)
Short name:
MHCK-B
Gene namesi
Name:mhkB
Synonyms:mhckB
ORF Names:DDB_G0289115
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0289115. mhkB.

Subcellular locationi

GO - Cellular componenti

  • actomyosin contractile ring Source: dictyBase
  • cytoplasm Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510481 – 732Myosin heavy chain kinase BAdd BLAST732

Proteomic databases

PaxDbiP90648.

Interactioni

GO - Molecular functioni

  • myosin II binding Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0191333.

Structurei

3D structure databases

ProteinModelPortaliP90648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini124 – 328Alpha-type protein kinasePROSITE-ProRule annotationAdd BLAST205
Repeati458 – 486WD 1Add BLAST29
Repeati500 – 528WD 2Add BLAST29
Repeati540 – 568WD 3Add BLAST29
Repeati580 – 608WD 4Add BLAST29
Repeati620 – 648WD 5Add BLAST29
Repeati660 – 688WD 6Add BLAST29
Repeati700 – 730WD 7Add BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi355 – 381Poly-AsnAdd BLAST27

Sequence similaritiesi

Contains 1 alpha-type protein kinase domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IQC6. Eukaryota.
ENOG410YE3S. LUCA.
InParanoidiP90648.
OMAiNDERNTP.
PhylomeDBiP90648.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02816. Alpha_kinase. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00811. Alpha_kinase. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
PS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P90648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFKVWFSYE DEEVELSELT NDTTVSAIRK ILHEGKIFRF PYGTSQTDLQ
60 70 80 90 100
IGKMLPSGSG GGATADSKFE KFKARNTLAD IQYKVGDTLY VRVKKSKPTN
110 120 130 140 150
DSLLPTLNIA FLDGSERAIK WEYDPYTTTA QWTCTATLVK VEPVPFAEGA
160 170 180 190 200
FRKAYHTLDL SKSGASGRYV SKIGKKPTPR PSYFEDVKMQ MIAKKWADKY
210 220 230 240 250
NSFKPPKKIE FLQSCVLEFV DRTSSDLICG AEPYVEGQYR KYNNNSGFVS
260 270 280 290 300
NDERNTPQSF SHFTYEHSNH QLLIIDIQGV GDHYTDPQIH TYDGVGFGIG
310 320 330 340 350
NLGQKGFEKF LDTHKCNAIC QYLNLQSINP KSEKSDCGTV PRPDLIFPDT
360 370 380 390 400
SERDNNNNNN NNNNNNNNNN NNNSNNNNNN NSSISKSLVE ISSGSKERND
410 420 430 440 450
RDSPSRQLFV SNDGNTLNTN KERSKSKSID LEKPEILINN KKKESINLET
460 470 480 490 500
IKLIETIKGY HVTSHLCICD NLLFTGCSDN SIRVYDYKSQ NMECVQTLKG
510 520 530 540 550
HEGPVESICY NDQYLFSGSS DHSIKVWDLK KLRCIFTLEG HDKPVHTVLL
560 570 580 590 600
NDKYLFSGSS DKTIKVWDLK TLECKYTLES HARAVKTLCI SGQYLFSGSN
610 620 630 640 650
DKTIKVWDLK TFRCNYTLKG HTKWVTTICI LGTNLYSGSY DKTIRVWNLK
660 670 680 690 700
SLECSATLRG HDRWVEHMVI CDKLLFTASD DNTIKIWDLE TLRCNTTLEG
710 720 730
HNATVQCLAV WEDKKCVISC SHDQSIRVWG WN
Length:732
Mass (Da):83,168
Last modified:May 1, 1997 - v1
Checksum:iA7233C4BD56D4088
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90946 mRNA. Translation: AAB50136.1.
AAFI02000130 Genomic DNA. Translation: EAL62865.1.
RefSeqiXP_636368.1. XM_631276.1.

Genome annotation databases

EnsemblProtistsiEAL62865; EAL62865; DDB_G0289115.
GeneIDi8626969.
KEGGiddi:DDB_G0289115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90946 mRNA. Translation: AAB50136.1.
AAFI02000130 Genomic DNA. Translation: EAL62865.1.
RefSeqiXP_636368.1. XM_631276.1.

3D structure databases

ProteinModelPortaliP90648.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191333.

Proteomic databases

PaxDbiP90648.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL62865; EAL62865; DDB_G0289115.
GeneIDi8626969.
KEGGiddi:DDB_G0289115.

Organism-specific databases

dictyBaseiDDB_G0289115. mhkB.

Phylogenomic databases

eggNOGiENOG410IQC6. Eukaryota.
ENOG410YE3S. LUCA.
InParanoidiP90648.
OMAiNDERNTP.
PhylomeDBiP90648.

Enzyme and pathway databases

BRENDAi2.7.11.7. 1939.

Miscellaneous databases

PROiP90648.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02816. Alpha_kinase. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00811. Alpha_kinase. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
PS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMHCKB_DICDI
AccessioniPrimary (citable) accession number: P90648
Secondary accession number(s): Q54HZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.