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P90551 (GPDA_LEIME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], glycosomal

EC=1.1.1.8
Gene names
Name:GPD
OrganismLeishmania mexicana
Taxonomic identifier5665 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH. HAMAP-Rule MF_00394

Subunit structure

Homodimer. Ref.2

Subcellular location

Glycosome Ref.1.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366Glycerol-3-phosphate dehydrogenase [NAD(+)], glycosomal HAMAP-Rule MF_00394
PRO_0000262292

Regions

Nucleotide binding22 – 276NAD HAMAP-Rule MF_00394
Region274 – 2752Substrate binding HAMAP-Rule MF_00394
Motif364 – 3663Microbody targeting signal Potential

Sites

Active site2101Proton acceptor Probable
Binding site971NAD By similarity
Binding site1251NAD; via amide nitrogen
Binding site1251Substrate
Binding site1571NAD; via amide nitrogen
Binding site2741NAD By similarity
Binding site2981NAD; via amide nitrogen
Binding site3001NAD

Experimental info

Mutagenesis1251K → A or M: Loss of activity. Ref.4
Mutagenesis2101K → A or M: Loss of activity. Ref.4

Secondary structure

........................................................... 366
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P90551 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: E47594525FC0CF44

FASTA36639,272
        10         20         30         40         50         60 
MSTKQHSAKD ELLYLNKAVV FGSGAFGTAL AMVLSKKCRE VCVWHMNEEE VRLVNEKREN 

        70         80         90        100        110        120 
VLFLKGVQLA SNITFTSDVE KAYNGAEIIL FVIPTQFLRG FFEKSGGNLI AYAKEKQVPV 

       130        140        150        160        170        180 
LVCTKGIERS TLKFPAEIIG EFLPSPLLSV LAGPSFAIEV ATGVFTCVSI ASADINVARR 

       190        200        210        220        230        240 
LQRIMSTGDR SFVCWATTDT VGCEVASAVK NVLAIGSGVA NGLGMGLNAR AALIMRGLLE 

       250        260        270        280        290        300 
IRDLTAALGG DGSAVFGLAG LGDLQLTCSS ELSRNFTVGK KLGKGLPIEE IQRTSKAVAE 

       310        320        330        340        350        360 
GVATADPLMR LAKQLKVKMP LCHQIYEIVY KKKNPRDALA DLLSCGLQDE GLPPLFKRSA 


STPSKL 

« Hide

References

[1]"Cloning and characterization of the NAD-linked glycerol-3-phosphate dehydrogenases of Trypanosoma brucei brucei and Leishmania mexicana mexicana and expression of the trypanosome enzyme in Escherichia coli."
Kohl L., Drmota T., Thi C.-D., Callens M., van Beeumen J., Opperdoes F.R., Michels P.A.M.
Mol. Biochem. Parasitol. 76:159-173(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[2]"A potential target enzyme for trypanocidal drugs revealed by the crystal structure of NAD-dependent glycerol-3-phosphate dehydrogenase from Leishmania mexicana."
Suresh S., Turley S., Opperdoes F.R., Michels P.A.M., Hol W.G.J.
Structure 8:541-552(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) IN COMPLEX WITH NAD AND SUBSTRATE ANALOG, SUBUNIT.
[3]"Anomalous differences of light elements in determining precise binding modes of ligands to glycerol-3-phosphate dehydrogenase."
Choe J., Suresh S., Wisedchaisri G., Kennedy K.J., Gelb M.H., Hol W.G.J.
Chem. Biol. 9:1189-1197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH NAD ANALOG.
[4]"Leishmania mexicana glycerol-3-phosphate dehydrogenase showed conformational changes upon binding a bi-substrate adduct."
Choe J., Guerra D., Michels P.A.M., Hol W.G.J.
J. Mol. Biol. 329:335-349(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND NAD, MUTAGENESIS OF LYS-125 AND LYS-210.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89739 Genomic DNA. Translation: CAA61891.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EVYX-ray1.75A1-366[»]
1EVZX-ray2.80A1-366[»]
1JDJX-ray2.20A1-366[»]
1M66X-ray1.90A1-366[»]
1M67X-ray2.50A1-366[»]
1N1EX-ray1.90A/B1-366[»]
1N1GX-ray2.50A1-366[»]
ProteinModelPortalP90551.
SMRP90551. Positions 9-357.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP90551.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_00394. NAD_Glyc3P_dehydrog.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSPR00077. GPDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
PROSITEPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP90551.

Entry information

Entry nameGPDA_LEIME
AccessionPrimary (citable) accession number: P90551
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: May 1, 1997
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references