Reviewed, UniProtKB/Swiss-Prot P90522 (ALG1_DICDI)

Names and origin

Protein names

Recommended name:
    Chitobiosyldiphosphodolichol beta-mannosyltransferase
    EC=2.4.1.142
Alternative name(s):
    GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
    GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
    Beta-1,4-mannosyltransferase
    Asparagine-linked glycosylation protein 1 homolog

Gene names

Name:	alg1
Synonyms:	mntA
ORF Names:	DDB_G0286011

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER.
Catalytic activity	GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Developmental stage	In bacterially grown celles expressed at 2 hours and 12 hours of development. In axenically grown cells expressed at 2 hours and 8/9 hours of development. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitobiosyldiphosphodolichol beta-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Chitobiosyldiphosphodolichol beta-mannosyltransferase		PRO_0000326038
Regions
	Topological domain	1 – 71	71	Cytoplasmic Potential
	Transmembrane	72 – 92	21	Signal-anchor for type II membrane protein Potential
	Topological domain	93 – 493	401	Extracellular Potential
	Compositional bias	465 – 490	26	Ser-rich
Amino acid modifications
	Glycosylation	359	1	N-linked (GlcNAc...) Potential
	Glycosylation	421	1	N-linked (GlcNAc...) Potential
	Glycosylation	441	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P90522-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 86279C007578BC02	FASTA	493	56,010
	10 20 30 40 50 60 MNRVAVVVLG DIGRSPRMQY HSMSLSKLEN TKVTLIGYRE SEPHPQIVNN DSITIEPLKP 70 80 90 100 110 120 FPISMSNSFK KIPLISIFMW PLLAICKVLF QIIQLMYVLL VKVPSPLNTI LVQSPPAIPT 130 140 150 160 170 180 IFVMQIVCWI RGVHLVIDWH NLGYTLLKLS LSKSDNHPII RLAKFIERYF AKNAYAHLFV 190 200 210 220 230 240 TNEMKIQLVR DWNLKGKTFV FHDKASPIFK SLTDREQEEF LKTFINKYSI KGEDKVYIES 250 260 270 280 290 300 VISKKSIRNP KQQTSIIISS TSWTQDEDFS ILLDAIVKYD IEHAINNNNN KVEEAQDESV 310 320 330 340 350 360 VLAENLLFII TGKGPQKEYY QEKINSLSLK KSRIITVWLD SEDYPKLLAC CDLGVSLHNS 370 380 390 400 410 420 SSGIDLPMKV VDMFGCCLPV LAIDFKCIGE LVKVNYNGFL FKDSDQLHQL LNQLFTHPTN 430 440 450 460 470 480 NNTITNTNNN KNLILEKMRK NLTKDRETDT WESNWLTIKP LFIPSSSSSS SSSSSSSSSS 490 SSSSSSNSKS KKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Dictyostelium discoideum beta-1,4-mannosyltransferase gene, mntA, has two periods of developmental expression." Lee S.-K., Li G., Yu S.-L., Alexander H., Alexander S. Gene 204:251-258(1997) [PubMed: 9434191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U65018 Genomic DNA. Translation: AAC47828.1. AAFI02000085 Genomic DNA. Translation: EAL64384.1.
RefSeq	XP_637897.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P90522.
Protein family/group databases
CAZy	GT33. Glycosyltransferase Family 33.
Genome annotation databases
GeneID	8625407.
KEGG	ddi:DDB_0191150.
Organism-specific databases
dictyBase	DDB_G0286011. alg1.
Phylogenomic databases
eggNOG	KOG2941.
HOGENOM	HBG319770.
OMA	TEISSEG.
PhylomeDB	P90522.
Enzyme and pathway databases
BRENDA	2.4.1.142. 424.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG1_DICDI

Accession

Primary (citable) accession number: P90522
Secondary accession number(s): Q54MD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 1, 1997
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)