Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P90521

- PFKA_DICDI

UniProt

P90521 - PFKA_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.2 PublicationsUniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 PublicationUniRule annotation

Cofactori

Mg2+1 PublicationUniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Kineticsi

  1. KM=16 µM for ATP1 Publication
  2. KM=22 µM for fructose 6-phosphate1 Publication

Vmax=89 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.6.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621ATP; via amide nitrogenUniRule annotation
Metal bindingi154 – 1541Magnesium; catalyticUniRule annotation
Active sitei201 – 2011Proton acceptorUniRule annotation
Binding sitei236 – 2361Substrate; shared with dimeric partnerUniRule annotation
Binding sitei299 – 2991SubstrateUniRule annotation
Binding sitei326 – 3261Substrate; shared with dimeric partnerUniRule annotation
Binding sitei507 – 5071Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei603 – 6031Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei666 – 6661Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei692 – 6921Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei764 – 7641Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi123 – 1242ATPUniRule annotation
Nucleotide bindingi153 – 1564ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: dictyBase
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. tubulin binding Source: dictyBase

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: dictyBase
  3. glucose catabolic process Source: dictyBase
  4. glycolytic process Source: UniProtKB-UniPathway
  5. negative regulation of microtubule polymerization Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.11. 1939.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkA
Synonyms:pfk
ORF Names:DDB_G0274111
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274111. pfkA.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: dictyBase
  2. cytosol Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 834834ATP-dependent 6-phosphofructokinasePRO_0000112030Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PRIDEiP90521.

Interactioni

Subunit structurei

Homotetramer.1 PublicationUniRule annotation

Protein-protein interaction databases

STRINGi44689.DDB_0191364.

Structurei

3D structure databases

ProteinModelPortaliP90521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 426426N-terminal catalytic PFK domain 1Add
BLAST
Regioni199 – 2013Substrate bindingUniRule annotation
Regioni243 – 2453Substrate bindingUniRule annotation
Regioni332 – 3354Substrate bindingUniRule annotation
Regioni427 – 43711Interdomain linkerAdd
BLAST
Regioni438 – 834397C-terminal regulatory PFK domain 2Add
BLAST
Regioni566 – 5705Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni610 – 6123Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni698 – 7014Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
InParanoidiP90521.
KOiK00850.
OMAiERSYIME.
PhylomeDBiP90521.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P90521-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTTSKIIND GEGEDVKGNK NINKKSLIDE NRLDEKKDLL SDKVESKTHC
60 70 80 90 100
SVKRMAVLTS GGDSSGMNPA IRAFARQVML KGAKVFAVRE GYNGLVNDSI
110 120 130 140 150
VPLNWGSVAG IISRGGTIIG TARSAEFRTR EGRKRAVFNL VKNRIDNLLV
160 170 180 190 200
IGGDGSLTGA NLLRTEWCSL LEELVKDGKL TLDVMEHFPI LSIAGIVGSI
210 220 230 240 250
DNDMCGTDLT VGADTATKRI LEAIDSILST AVSHQRSFVI EVMGRNCGWL
260 270 280 290 300
ALASGVATGA DYILIPESPP DDGWEQTMAD NLERGRLSGR RCSLVIVSEG
310 320 330 340 350
AIDRQGKPIT SAYVRQFLED KGHDARITIL GHVQRGGTPT FLDRYIATRM
360 370 380 390 400
GIEAANYFYD STIEQLKQPV LIGMSGMDTI RSPLMECVQK TQSIASLIKE
410 420 430 440 450
RRFNEVVDVR GGMFKEFYEI FIACSNLHRR KVESKGMGVL ILHSGGPSPG
460 470 480 490 500
MNPCVRAFTR LGIDHGYTMY GCFNGFGGLA LGEIEQLHWM TVNGWSVMGG
510 520 530 540 550
AELGTNRSIP NDSNIEAIIA TLERFKINAI LMFGGFNGYL GIAKLYEYRE
560 570 580 590 600
KYQQLKRISI IGAPGTIANN VPGTNISIGS DTSLNNTLDA LDKIKQSAVA
610 620 630 640 650
SRRLFVVEVM GAHCGYLAAM SSLTSGAERS YIMERGITLN TLTKDLEMFV
660 670 680 690 700
ERFKREHRIG LIIKSELASN TYSTHFIYSL FKEEGKHLFD VRESILGHLQ
710 720 730 740 750
QGGTPSAIDR IFSTRLMNHY YQFLENDLKE HGHLQMNGCI GFIDGGIHYT
760 770 780 790 800
PMQEMIEEMS DKFRRPRSQW WMDLVETSQN ISVFPLDDPS STNFEGCNSN
810 820 830
LSEQDRPIKK SDISSPTSYS QKTFDPNVNP QFTL
Length:834
Mass (Da):92,235
Last modified:December 4, 2007 - v2
Checksum:iF9E4F2DF798FE78B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1123GII → WLY in CAA61438. (PubMed:9030771)Curated
Sequence conflicti110 – 1123GII → WLY in CAA64065. (PubMed:9030771)Curated
Sequence conflicti302 – 3032ID → MH in CAA64065. (PubMed:9030771)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89039 mRNA. Translation: CAA61438.2.
X94340 mRNA. Translation: CAA64065.1.
AAFI02000012 Genomic DNA. Translation: EAL69948.1.
RefSeqiXP_644162.1. XM_639070.1.

Genome annotation databases

EnsemblProtistsiDDB0191364; DDB0191364; DDB_G0274111.
GeneIDi8619591.
KEGGiddi:DDB_G0274111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89039 mRNA. Translation: CAA61438.2 .
X94340 mRNA. Translation: CAA64065.1 .
AAFI02000012 Genomic DNA. Translation: EAL69948.1 .
RefSeqi XP_644162.1. XM_639070.1.

3D structure databases

ProteinModelPortali P90521.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191364.

Proteomic databases

PRIDEi P90521.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191364 ; DDB0191364 ; DDB_G0274111 .
GeneIDi 8619591.
KEGGi ddi:DDB_G0274111.

Organism-specific databases

dictyBasei DDB_G0274111. pfkA.

Phylogenomic databases

eggNOGi COG0205.
InParanoidi P90521.
KOi K00850.
OMAi ERSYIME.
PhylomeDBi P90521.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BRENDAi 2.7.1.11. 1939.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and developmental expression of phosphofructokinase from Dictyostelium discoideum."
    Estevez A.M., Martinez-Costa O.H., Sanchez V., Aragon J.J.
    Eur. J. Biochem. 243:442-451(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AX3.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "Purification and properties of phosphofructokinase from Dictyostelium discoideum."
    Martinez-Costa O.H., Estevez A.M., Sanchez V., Aragon J.J.
    Eur. J. Biochem. 226:1007-1017(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, COFACTOR.
  5. "Distinct functional roles of the two terminal halves of eukaryotic phosphofructokinase."
    Martinez-Costa O.H., Sanchez V., Lazaro A., Hernandez E.D., Tornheim K., Aragon J.J.
    Biochem. J. 445:213-218(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPFKA_DICDI
AccessioniPrimary (citable) accession number: P90521
Secondary accession number(s): Q555B1, Q86HR6, Q95023
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 4, 2007
Last modified: November 26, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3