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P90521

- PFKA_DICDI

UniProt

P90521 - PFKA_DICDI

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, pfk, DDB_G0274111
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.2 Publications

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 Publication

Cofactori

Magnesium.1 Publication

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Kineticsi

  1. KM=16 µM for ATP1 Publication
  2. KM=22 µM for fructose 6-phosphate

Vmax=89 µmol/min/mg enzyme

pH dependencei

Optimum pH is 7.6.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621ATP; via amide nitrogen By similarity
Metal bindingi154 – 1541Magnesium; catalytic By similarity
Active sitei201 – 2011Proton acceptor By similarity
Binding sitei236 – 2361Substrate; shared with dimeric partner By similarity
Binding sitei299 – 2991Substrate By similarity
Binding sitei326 – 3261Substrate; shared with dimeric partner By similarity
Binding sitei507 – 5071Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei603 – 6031Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei666 – 6661Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei692 – 6921Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei764 – 7641Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi123 – 1242ATP By similarity
Nucleotide bindingi153 – 1564ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: dictyBase
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. tubulin binding Source: dictyBase

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: dictyBase
  3. glucose catabolic process Source: dictyBase
  4. glycolytic process Source: UniProtKB-UniPathway
  5. negative regulation of microtubule polymerization Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.11. 1939.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Synonyms:pfk
ORF Names:DDB_G0274111
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274111. pfkA.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: dictyBase
  2. cytosol Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 834834ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000112030Add
BLAST

Post-translational modificationi

The N-terminus is blocked.UniRule annotation

Proteomic databases

PRIDEiP90521.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB_0191364.

Structurei

3D structure databases

ProteinModelPortaliP90521.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 426426N-terminal catalytic PFK domain 1UniRule annotationAdd
BLAST
Regioni199 – 2013Substrate binding By similarity
Regioni243 – 2453Substrate binding By similarity
Regioni332 – 3354Substrate binding By similarity
Regioni427 – 43711Interdomain linkerUniRule annotationAdd
BLAST
Regioni438 – 834397C-terminal regulatory PFK domain 2UniRule annotationAdd
BLAST
Regioni566 – 5705Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni610 – 6123Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni698 – 7014Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
KOiK00850.
OMAiERSYIME.
PhylomeDBiP90521.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P90521-1 [UniParc]FASTAAdd to Basket

« Hide

MTTTSKIIND GEGEDVKGNK NINKKSLIDE NRLDEKKDLL SDKVESKTHC    50
SVKRMAVLTS GGDSSGMNPA IRAFARQVML KGAKVFAVRE GYNGLVNDSI 100
VPLNWGSVAG IISRGGTIIG TARSAEFRTR EGRKRAVFNL VKNRIDNLLV 150
IGGDGSLTGA NLLRTEWCSL LEELVKDGKL TLDVMEHFPI LSIAGIVGSI 200
DNDMCGTDLT VGADTATKRI LEAIDSILST AVSHQRSFVI EVMGRNCGWL 250
ALASGVATGA DYILIPESPP DDGWEQTMAD NLERGRLSGR RCSLVIVSEG 300
AIDRQGKPIT SAYVRQFLED KGHDARITIL GHVQRGGTPT FLDRYIATRM 350
GIEAANYFYD STIEQLKQPV LIGMSGMDTI RSPLMECVQK TQSIASLIKE 400
RRFNEVVDVR GGMFKEFYEI FIACSNLHRR KVESKGMGVL ILHSGGPSPG 450
MNPCVRAFTR LGIDHGYTMY GCFNGFGGLA LGEIEQLHWM TVNGWSVMGG 500
AELGTNRSIP NDSNIEAIIA TLERFKINAI LMFGGFNGYL GIAKLYEYRE 550
KYQQLKRISI IGAPGTIANN VPGTNISIGS DTSLNNTLDA LDKIKQSAVA 600
SRRLFVVEVM GAHCGYLAAM SSLTSGAERS YIMERGITLN TLTKDLEMFV 650
ERFKREHRIG LIIKSELASN TYSTHFIYSL FKEEGKHLFD VRESILGHLQ 700
QGGTPSAIDR IFSTRLMNHY YQFLENDLKE HGHLQMNGCI GFIDGGIHYT 750
PMQEMIEEMS DKFRRPRSQW WMDLVETSQN ISVFPLDDPS STNFEGCNSN 800
LSEQDRPIKK SDISSPTSYS QKTFDPNVNP QFTL 834
Length:834
Mass (Da):92,235
Last modified:December 4, 2007 - v2
Checksum:iF9E4F2DF798FE78B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1123GII → WLY in CAA61438. 1 Publication
Sequence conflicti110 – 1123GII → WLY in CAA64065. 1 Publication
Sequence conflicti302 – 3032ID → MH in CAA64065. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89039 mRNA. Translation: CAA61438.2.
X94340 mRNA. Translation: CAA64065.1.
AAFI02000012 Genomic DNA. Translation: EAL69948.1.
RefSeqiXP_644162.1. XM_639070.1.

Genome annotation databases

EnsemblProtistsiDDB0191364; DDB0191364; DDB_G0274111.
GeneIDi8619591.
KEGGiddi:DDB_G0274111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89039 mRNA. Translation: CAA61438.2 .
X94340 mRNA. Translation: CAA64065.1 .
AAFI02000012 Genomic DNA. Translation: EAL69948.1 .
RefSeqi XP_644162.1. XM_639070.1.

3D structure databases

ProteinModelPortali P90521.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191364.

Proteomic databases

PRIDEi P90521.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191364 ; DDB0191364 ; DDB_G0274111 .
GeneIDi 8619591.
KEGGi ddi:DDB_G0274111.

Organism-specific databases

dictyBasei DDB_G0274111. pfkA.

Phylogenomic databases

eggNOGi COG0205.
KOi K00850.
OMAi ERSYIME.
PhylomeDBi P90521.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BRENDAi 2.7.1.11. 1939.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and developmental expression of phosphofructokinase from Dictyostelium discoideum."
    Estevez A.M., Martinez-Costa O.H., Sanchez V., Aragon J.J.
    Eur. J. Biochem. 243:442-451(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AX3.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "Purification and properties of phosphofructokinase from Dictyostelium discoideum."
    Martinez-Costa O.H., Estevez A.M., Sanchez V., Aragon J.J.
    Eur. J. Biochem. 226:1007-1017(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, COFACTOR.
  5. "Distinct functional roles of the two terminal halves of eukaryotic phosphofructokinase."
    Martinez-Costa O.H., Sanchez V., Lazaro A., Hernandez E.D., Tornheim K., Aragon J.J.
    Biochem. J. 445:213-218(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPFKA_DICDI
AccessioniPrimary (citable) accession number: P90521
Secondary accession number(s): Q555B1, Q86HR6, Q95023
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi