P90521 (K6PF_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Synonyms:	pfk
ORF Names:	DDB_G0274111

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from direct assay PubMed 7813455. Source: dictyBase glucose catabolic process Inferred from genetic interaction PubMed 7589492. Source: dictyBase glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway negative regulation of microtubule polymerization Inferred from direct assay PubMed 10026266. Source: dictyBase
Cellular_component	6-phosphofructokinase complex Inferred from direct assay PubMed 7813455. Source: dictyBase
Molecular_function	6-phosphofructokinase activity Inferred from direct assay PubMed 4300706PubMed 7813455. Source: dictyBase ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	6-phosphofructokinase		PRO_0000112030
Regions
	Nucleotide binding	72 – 76	5	ATP By similarity
	Nucleotide binding	228 – 232	5	ATP By similarity
	Nucleotide binding	245 – 261	17	ATP By similarity
Sites
	Active site	201	1	Proton acceptor By similarity
	Metal binding	259	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	236	1	Substrate By similarity
	Binding site	326	1	Substrate By similarity
	Binding site	332	1	Substrate By similarity
	Binding site	335	1	Substrate By similarity
Experimental info
	Sequence conflict	110 – 112	3	GII → WLY in CAA61438. Ref.1
	Sequence conflict	110 – 112	3	GII → WLY in CAA64065. Ref.1
	Sequence conflict	302 – 303	2	ID → MH in CAA64065. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P90521 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: F9E4F2DF798FE78B	FASTA	834	92,235
	10 20 30 40 50 60 MTTTSKIIND GEGEDVKGNK NINKKSLIDE NRLDEKKDLL SDKVESKTHC SVKRMAVLTS 70 80 90 100 110 120 GGDSSGMNPA IRAFARQVML KGAKVFAVRE GYNGLVNDSI VPLNWGSVAG IISRGGTIIG 130 140 150 160 170 180 TARSAEFRTR EGRKRAVFNL VKNRIDNLLV IGGDGSLTGA NLLRTEWCSL LEELVKDGKL 190 200 210 220 230 240 TLDVMEHFPI LSIAGIVGSI DNDMCGTDLT VGADTATKRI LEAIDSILST AVSHQRSFVI 250 260 270 280 290 300 EVMGRNCGWL ALASGVATGA DYILIPESPP DDGWEQTMAD NLERGRLSGR RCSLVIVSEG 310 320 330 340 350 360 AIDRQGKPIT SAYVRQFLED KGHDARITIL GHVQRGGTPT FLDRYIATRM GIEAANYFYD 370 380 390 400 410 420 STIEQLKQPV LIGMSGMDTI RSPLMECVQK TQSIASLIKE RRFNEVVDVR GGMFKEFYEI 430 440 450 460 470 480 FIACSNLHRR KVESKGMGVL ILHSGGPSPG MNPCVRAFTR LGIDHGYTMY GCFNGFGGLA 490 500 510 520 530 540 LGEIEQLHWM TVNGWSVMGG AELGTNRSIP NDSNIEAIIA TLERFKINAI LMFGGFNGYL 550 560 570 580 590 600 GIAKLYEYRE KYQQLKRISI IGAPGTIANN VPGTNISIGS DTSLNNTLDA LDKIKQSAVA 610 620 630 640 650 660 SRRLFVVEVM GAHCGYLAAM SSLTSGAERS YIMERGITLN TLTKDLEMFV ERFKREHRIG 670 680 690 700 710 720 LIIKSELASN TYSTHFIYSL FKEEGKHLFD VRESILGHLQ QGGTPSAIDR IFSTRLMNHY 730 740 750 760 770 780 YQFLENDLKE HGHLQMNGCI GFIDGGIHYT PMQEMIEEMS DKFRRPRSQW WMDLVETSQN 790 800 810 820 830 ISVFPLDDPS STNFEGCNSN LSEQDRPIKK SDISSPTSYS QKTFDPNVNP QFTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and developmental expression of phosphofructokinase from Dictyostelium discoideum." Estevez A.M., Martinez-Costa O.H., Sanchez V., Aragon J.J. Eur. J. Biochem. 243:442-451(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX3.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89039 mRNA. Translation: CAA61438.2. X94340 mRNA. Translation: CAA64065.1. AAFI02000012 Genomic DNA. Translation: EAL69948.1.
RefSeq	XP_644162.1. XM_639070.1.
3D structure databases
ProteinModelPortal	P90521.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191364.
Proteomic databases
PRIDE	P90521.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191364; DDB0191364; DDB_G0274111.
GeneID	8619591.
KEGG	ddi:DDB_G0274111.
Organism-specific databases
dictyBase	DDB_G0274111. pfkA.
Phylogenomic databases
eggNOG	COG0205.
KO	K00850.
OMA	RESILGH.
ProtClustDB	CLSZ2431034.
Enzyme and pathway databases
BRENDA	2.7.1.11. 1939.
UniPathway	UPA00109; UER00182.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_DICDI

Accession

Primary (citable) accession number: P90521
Secondary accession number(s): Q555B1, Q86HR6, Q95023

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)