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P90521

- PFKA_DICDI

UniProt

P90521 - PFKA_DICDI

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.2 PublicationsUniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 PublicationUniRule annotation

    Cofactori

    Magnesium.1 PublicationUniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Kineticsi

    1. KM=16 µM for ATP1 Publication
    2. KM=22 µM for fructose 6-phosphate1 Publication

    Vmax=89 µmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 7.6.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei62 – 621ATP; via amide nitrogenUniRule annotation
    Metal bindingi154 – 1541Magnesium; catalyticUniRule annotation
    Active sitei201 – 2011Proton acceptorUniRule annotation
    Binding sitei236 – 2361Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei299 – 2991SubstrateUniRule annotation
    Binding sitei326 – 3261Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei507 – 5071Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei603 – 6031Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei666 – 6661Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei692 – 6921Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei764 – 7641Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi123 – 1242ATPUniRule annotation
    Nucleotide bindingi153 – 1564ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: dictyBase
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. tubulin binding Source: dictyBase

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 6-phosphate metabolic process Source: dictyBase
    3. glucose catabolic process Source: dictyBase
    4. glycolytic process Source: UniProtKB-UniPathway
    5. negative regulation of microtubule polymerization Source: dictyBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.11. 1939.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkA
    Synonyms:pfk
    ORF Names:DDB_G0274111
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0274111. pfkA.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: dictyBase
    2. cytosol Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 834834ATP-dependent 6-phosphofructokinasePRO_0000112030Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    PRIDEiP90521.

    Interactioni

    Subunit structurei

    Homotetramer.1 PublicationUniRule annotation

    Protein-protein interaction databases

    STRINGi44689.DDB_0191364.

    Structurei

    3D structure databases

    ProteinModelPortaliP90521.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 426426N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni199 – 2013Substrate bindingUniRule annotation
    Regioni243 – 2453Substrate bindingUniRule annotation
    Regioni332 – 3354Substrate bindingUniRule annotation
    Regioni427 – 43711Interdomain linkerAdd
    BLAST
    Regioni438 – 834397C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni566 – 5705Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni610 – 6123Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni698 – 7014Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    KOiK00850.
    OMAiERSYIME.
    PhylomeDBiP90521.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P90521-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTTSKIIND GEGEDVKGNK NINKKSLIDE NRLDEKKDLL SDKVESKTHC    50
    SVKRMAVLTS GGDSSGMNPA IRAFARQVML KGAKVFAVRE GYNGLVNDSI 100
    VPLNWGSVAG IISRGGTIIG TARSAEFRTR EGRKRAVFNL VKNRIDNLLV 150
    IGGDGSLTGA NLLRTEWCSL LEELVKDGKL TLDVMEHFPI LSIAGIVGSI 200
    DNDMCGTDLT VGADTATKRI LEAIDSILST AVSHQRSFVI EVMGRNCGWL 250
    ALASGVATGA DYILIPESPP DDGWEQTMAD NLERGRLSGR RCSLVIVSEG 300
    AIDRQGKPIT SAYVRQFLED KGHDARITIL GHVQRGGTPT FLDRYIATRM 350
    GIEAANYFYD STIEQLKQPV LIGMSGMDTI RSPLMECVQK TQSIASLIKE 400
    RRFNEVVDVR GGMFKEFYEI FIACSNLHRR KVESKGMGVL ILHSGGPSPG 450
    MNPCVRAFTR LGIDHGYTMY GCFNGFGGLA LGEIEQLHWM TVNGWSVMGG 500
    AELGTNRSIP NDSNIEAIIA TLERFKINAI LMFGGFNGYL GIAKLYEYRE 550
    KYQQLKRISI IGAPGTIANN VPGTNISIGS DTSLNNTLDA LDKIKQSAVA 600
    SRRLFVVEVM GAHCGYLAAM SSLTSGAERS YIMERGITLN TLTKDLEMFV 650
    ERFKREHRIG LIIKSELASN TYSTHFIYSL FKEEGKHLFD VRESILGHLQ 700
    QGGTPSAIDR IFSTRLMNHY YQFLENDLKE HGHLQMNGCI GFIDGGIHYT 750
    PMQEMIEEMS DKFRRPRSQW WMDLVETSQN ISVFPLDDPS STNFEGCNSN 800
    LSEQDRPIKK SDISSPTSYS QKTFDPNVNP QFTL 834
    Length:834
    Mass (Da):92,235
    Last modified:December 4, 2007 - v2
    Checksum:iF9E4F2DF798FE78B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 1123GII → WLY in CAA61438. (PubMed:9030771)Curated
    Sequence conflicti110 – 1123GII → WLY in CAA64065. (PubMed:9030771)Curated
    Sequence conflicti302 – 3032ID → MH in CAA64065. (PubMed:9030771)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89039 mRNA. Translation: CAA61438.2.
    X94340 mRNA. Translation: CAA64065.1.
    AAFI02000012 Genomic DNA. Translation: EAL69948.1.
    RefSeqiXP_644162.1. XM_639070.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191364; DDB0191364; DDB_G0274111.
    GeneIDi8619591.
    KEGGiddi:DDB_G0274111.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89039 mRNA. Translation: CAA61438.2 .
    X94340 mRNA. Translation: CAA64065.1 .
    AAFI02000012 Genomic DNA. Translation: EAL69948.1 .
    RefSeqi XP_644162.1. XM_639070.1.

    3D structure databases

    ProteinModelPortali P90521.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191364.

    Proteomic databases

    PRIDEi P90521.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191364 ; DDB0191364 ; DDB_G0274111 .
    GeneIDi 8619591.
    KEGGi ddi:DDB_G0274111.

    Organism-specific databases

    dictyBasei DDB_G0274111. pfkA.

    Phylogenomic databases

    eggNOGi COG0205.
    KOi K00850.
    OMAi ERSYIME.
    PhylomeDBi P90521.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BRENDAi 2.7.1.11. 1939.

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and developmental expression of phosphofructokinase from Dictyostelium discoideum."
      Estevez A.M., Martinez-Costa O.H., Sanchez V., Aragon J.J.
      Eur. J. Biochem. 243:442-451(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: AX3.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "Purification and properties of phosphofructokinase from Dictyostelium discoideum."
      Martinez-Costa O.H., Estevez A.M., Sanchez V., Aragon J.J.
      Eur. J. Biochem. 226:1007-1017(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, COFACTOR.
    5. "Distinct functional roles of the two terminal halves of eukaryotic phosphofructokinase."
      Martinez-Costa O.H., Sanchez V., Lazaro A., Hernandez E.D., Tornheim K., Aragon J.J.
      Biochem. J. 445:213-218(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPFKA_DICDI
    AccessioniPrimary (citable) accession number: P90521
    Secondary accession number(s): Q555B1, Q86HR6, Q95023
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3