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P90521 (PFKA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Synonyms:pfk
ORF Names:DDB_G0274111
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Ref.4 Ref.5

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. Ref.4

Cofactor

Magnesium. Ref.4

Enzyme regulation

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity. HAMAP-Rule MF_03184

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_03184

Subunit structure

Homotetramer. Ref.4

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_03184.

Post-translational modification

The N-terminus is blocked. HAMAP-Rule MF_03184

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=16 µM for ATP Ref.4

KM=22 µM for fructose 6-phosphate

Vmax=89 µmol/min/mg enzyme

pH dependence:

Optimum pH is 7.6.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 834834ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_03184
PRO_0000112030

Regions

Nucleotide binding123 – 1242ATP By similarity
Nucleotide binding153 – 1564ATP By similarity
Region1 – 426426N-terminal catalytic PFK domain 1 HAMAP-Rule MF_03184
Region199 – 2013Substrate binding By similarity
Region243 – 2453Substrate binding By similarity
Region332 – 3354Substrate binding By similarity
Region427 – 43711Interdomain linker HAMAP-Rule MF_03184
Region438 – 834397C-terminal regulatory PFK domain 2 HAMAP-Rule MF_03184
Region566 – 5705Allosteric activator fructose 2,6-bisphosphate binding By similarity
Region610 – 6123Allosteric activator fructose 2,6-bisphosphate binding By similarity
Region698 – 7014Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sites

Active site2011Proton acceptor By similarity
Metal binding1541Magnesium; catalytic By similarity
Binding site621ATP; via amide nitrogen By similarity
Binding site2361Substrate; shared with dimeric partner By similarity
Binding site2991Substrate By similarity
Binding site3261Substrate; shared with dimeric partner By similarity
Binding site5071Allosteric activator fructose 2,6-bisphosphate By similarity
Binding site6031Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding site6661Allosteric activator fructose 2,6-bisphosphate By similarity
Binding site6921Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding site7641Allosteric activator fructose 2,6-bisphosphate By similarity

Experimental info

Sequence conflict110 – 1123GII → WLY in CAA61438. Ref.1
Sequence conflict110 – 1123GII → WLY in CAA64065. Ref.1
Sequence conflict302 – 3032ID → MH in CAA64065. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P90521 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: F9E4F2DF798FE78B

FASTA83492,235
        10         20         30         40         50         60 
MTTTSKIIND GEGEDVKGNK NINKKSLIDE NRLDEKKDLL SDKVESKTHC SVKRMAVLTS 

        70         80         90        100        110        120 
GGDSSGMNPA IRAFARQVML KGAKVFAVRE GYNGLVNDSI VPLNWGSVAG IISRGGTIIG 

       130        140        150        160        170        180 
TARSAEFRTR EGRKRAVFNL VKNRIDNLLV IGGDGSLTGA NLLRTEWCSL LEELVKDGKL 

       190        200        210        220        230        240 
TLDVMEHFPI LSIAGIVGSI DNDMCGTDLT VGADTATKRI LEAIDSILST AVSHQRSFVI 

       250        260        270        280        290        300 
EVMGRNCGWL ALASGVATGA DYILIPESPP DDGWEQTMAD NLERGRLSGR RCSLVIVSEG 

       310        320        330        340        350        360 
AIDRQGKPIT SAYVRQFLED KGHDARITIL GHVQRGGTPT FLDRYIATRM GIEAANYFYD 

       370        380        390        400        410        420 
STIEQLKQPV LIGMSGMDTI RSPLMECVQK TQSIASLIKE RRFNEVVDVR GGMFKEFYEI 

       430        440        450        460        470        480 
FIACSNLHRR KVESKGMGVL ILHSGGPSPG MNPCVRAFTR LGIDHGYTMY GCFNGFGGLA 

       490        500        510        520        530        540 
LGEIEQLHWM TVNGWSVMGG AELGTNRSIP NDSNIEAIIA TLERFKINAI LMFGGFNGYL 

       550        560        570        580        590        600 
GIAKLYEYRE KYQQLKRISI IGAPGTIANN VPGTNISIGS DTSLNNTLDA LDKIKQSAVA 

       610        620        630        640        650        660 
SRRLFVVEVM GAHCGYLAAM SSLTSGAERS YIMERGITLN TLTKDLEMFV ERFKREHRIG 

       670        680        690        700        710        720 
LIIKSELASN TYSTHFIYSL FKEEGKHLFD VRESILGHLQ QGGTPSAIDR IFSTRLMNHY 

       730        740        750        760        770        780 
YQFLENDLKE HGHLQMNGCI GFIDGGIHYT PMQEMIEEMS DKFRRPRSQW WMDLVETSQN 

       790        800        810        820        830 
ISVFPLDDPS STNFEGCNSN LSEQDRPIKK SDISSPTSYS QKTFDPNVNP QFTL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and developmental expression of phosphofructokinase from Dictyostelium discoideum."
Estevez A.M., Martinez-Costa O.H., Sanchez V., Aragon J.J.
Eur. J. Biochem. 243:442-451(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AX3.
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"Purification and properties of phosphofructokinase from Dictyostelium discoideum."
Martinez-Costa O.H., Estevez A.M., Sanchez V., Aragon J.J.
Eur. J. Biochem. 226:1007-1017(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, COFACTOR.
[5]"Distinct functional roles of the two terminal halves of eukaryotic phosphofructokinase."
Martinez-Costa O.H., Sanchez V., Lazaro A., Hernandez E.D., Tornheim K., Aragon J.J.
Biochem. J. 445:213-218(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89039 mRNA. Translation: CAA61438.2.
X94340 mRNA. Translation: CAA64065.1.
AAFI02000012 Genomic DNA. Translation: EAL69948.1.
RefSeqXP_644162.1. XM_639070.1.

3D structure databases

ProteinModelPortalP90521.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191364.

Proteomic databases

PRIDEP90521.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191364; DDB0191364; DDB_G0274111.
GeneID8619591.
KEGGddi:DDB_G0274111.

Organism-specific databases

dictyBaseDDB_G0274111. pfkA.

Phylogenomic databases

eggNOGCOG0205.
OMAERSYIME.
PhylomeDBP90521.

Enzyme and pathway databases

BRENDA2.7.1.11. 1939.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_03184. Phosphofructokinase_I_E.
InterProIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 2 hits.
[Graphical view]
PIRSFPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 2 hits.
TIGRFAMsTIGR02478. 6PF1K_euk. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_DICDI
AccessionPrimary (citable) accession number: P90521
Secondary accession number(s): Q555B1, Q86HR6, Q95023
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 4, 2007
Last modified: July 9, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase