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Protein

Glutathionylspermidine synthase

Gene

GSP

Organism
Crithidia fasciculata
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Conjugates glutathione (gamma-Glu-Cys-Gly) and spermidine to form glutathionylspermidine in the biosynthesis trypanothione (N1,N(8)-bis(glutathionyl)spermidine), which is involved in maintaining intracellular thiol redox and in defense against oxidants.

Catalytic activityi

Glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei350GlutathioneBy similarity1
Sitei350Transition state stabilizerBy similarity1
Metal bindingi352Magnesium 1By similarity1
Metal bindingi364Magnesium 1By similarity1
Metal bindingi364Magnesium 2By similarity1
Metal bindingi366Magnesium 2By similarity1
Binding sitei369GlutathioneBy similarity1
Binding sitei432SpermidineBy similarity1
Binding sitei433GlutathioneBy similarity1
Binding sitei501GlutathioneBy similarity1
Binding sitei544ATPBy similarity1
Binding sitei579ATPBy similarity1
Binding sitei586ATP; via amide nitrogenBy similarity1
Binding sitei653ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi350 – 352ATPBy similarity3
Nucleotide bindingi689 – 691ATPBy similarity3

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.8. 1365.
SABIO-RKP90518.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathionylspermidine synthase (EC:6.3.1.8)
Gene namesi
Name:GSP
OrganismiCrithidia fasciculata
Taxonomic identifieri5656 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeCrithidia

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3354.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000704421 – 719Glutathionylspermidine synthaseAdd BLAST719

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PRIDEiP90518.

Interactioni

Chemistry databases

BindingDBiP90518.

Structurei

3D structure databases

ProteinModelPortaliP90518.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 200Peptidase C51PROSITE-ProRule annotationAdd BLAST147

Sequence similaritiesi

In the C-terminal section; belongs to the glutathionylspermidine synthase preATP-grasp family.Curated
Contains 1 peptidase C51 domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK01917.

Family and domain databases

InterProiIPR007921. CHAP_dom.
IPR005494. GSPS_pre-ATP-grasp-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF05257. CHAP. 1 hit.
PF03738. GSP_synth. 2 hits.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS50911. CHAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P90518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLPHNHHY ETHHRGTAEV PFDELIGVTP DGVPVISNGN EAHFSNLESI
60 70 80 90 100
TAACLPLSSF ERKAPCKQPY RKMGVKWQCV EFVRRYLASR KAVWMTSLCT
110 120 130 140 150
AEEVWREENL FVDVRDGRPV EVVRTPNKST GPAPAVADIV VWGEGPETPF
160 170 180 190 200
GHVAIVTEVC ASCVRVAEQN QGFEKWPEDV PFSREIAMRT TESGEVELLD
210 220 230 240 250
EDPLLGWVTV QAPFYNFDDG DLADSFRLVV GQGQILRQPF PKHVDVPWLN
260 270 280 290 300
TGEECDTILK HSLVVDGNMG EGAHAEEGDV PGAFYFLDYD MFCRLGRAAS
310 320 330 340 350
SLHRIAMAAT AKVLEDPEST HLLEHYFGVP PEIQPLLRRS WEMTPPMGGR
360 370 380 390 400
FDFGYDGKNV VMLEYNCDSS GALLECCNTQ EKMARFYGVS QGTSTGSFLG
410 420 430 440 450
AKCVTYFQRL LTNEKVCPQH RLIHFMIDED DEERYTARCM MGFAEQAGFR
460 470 480 490 500
TKLCVKLVNF RYRDGPPSNA APLATPCDHP TIVDGEDEEV LMVWKTWSWD
510 520 530 540 550
TVLHQYHSQR SSSDAVNTPT LSDILLNNNI RVLEPLWKAV TGSKAILPFM
560 570 580 590 600
HALAPDHEHM LAASFLPTRE IISRHYISKP VNGRAGQNIM MYDPVTSPTE
610 620 630 640 650
LEGAPQQDIC EALSQNASAR SLLNGSPLPL SQSVDQTNEC SPGKFFDSVL
660 670 680 690 700
VYQQRLFLKK FDGKYFPIFC GWMVGDEFGG VVVREDTSKI TKLSSMVVPA
710
RVVRDNVPLG VSYSDEGET
Length:719
Mass (Da):80,322
Last modified:March 1, 2001 - v2
Checksum:i46069BE9C40EEEFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66520 Genomic DNA. Translation: AAC48361.2.

Genome annotation databases

KEGGiag:AAC48361.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66520 Genomic DNA. Translation: AAC48361.2.

3D structure databases

ProteinModelPortaliP90518.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP90518.
ChEMBLiCHEMBL3354.

Proteomic databases

PRIDEiP90518.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAC48361.

Phylogenomic databases

KOiK01917.

Enzyme and pathway databases

BRENDAi6.3.1.8. 1365.
SABIO-RKP90518.

Family and domain databases

InterProiIPR007921. CHAP_dom.
IPR005494. GSPS_pre-ATP-grasp-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF05257. CHAP. 1 hit.
PF03738. GSP_synth. 2 hits.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS50911. CHAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSP_CRIFA
AccessioniPrimary (citable) accession number: P90518
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: March 1, 2001
Last modified: October 5, 2016
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.