P90518 (GSP_CRIFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathionylspermidine synthase
EC=6.3.1.8

Gene names

Name:

GSP

Organism

Crithidia fasciculata

Taxonomic identifier

5656 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmaniinae › Crithidia

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugates glutathione (gamma-Glu-Cys-Gly) and spermidine to form glutathionylspermidine in the biosynthesis trypanothione (N1,N(8)-bis(glutathionyl)spermidine), which is involved in maintaining intracellular thiol redox and in defense against oxidants.
Catalytic activity	Glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate.
Cofactor	Magnesium.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Contains 1 peptidase C51 domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutathionylspermidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	Glutathionylspermidine synthase		PRO_0000070442
Regions
	Domain	54 – 200	147	Peptidase C51

Sequences

	Sequence		Length	Mass (Da)
	P90518 [UniParc]. Last modified March 1, 2001. Version 2. Checksum: 46069BE9C40EEEFC	FASTA	719	80,322
	10 20 30 40 50 60 MSSLPHNHHY ETHHRGTAEV PFDELIGVTP DGVPVISNGN EAHFSNLESI TAACLPLSSF 70 80 90 100 110 120 ERKAPCKQPY RKMGVKWQCV EFVRRYLASR KAVWMTSLCT AEEVWREENL FVDVRDGRPV 130 140 150 160 170 180 EVVRTPNKST GPAPAVADIV VWGEGPETPF GHVAIVTEVC ASCVRVAEQN QGFEKWPEDV 190 200 210 220 230 240 PFSREIAMRT TESGEVELLD EDPLLGWVTV QAPFYNFDDG DLADSFRLVV GQGQILRQPF 250 260 270 280 290 300 PKHVDVPWLN TGEECDTILK HSLVVDGNMG EGAHAEEGDV PGAFYFLDYD MFCRLGRAAS 310 320 330 340 350 360 SLHRIAMAAT AKVLEDPEST HLLEHYFGVP PEIQPLLRRS WEMTPPMGGR FDFGYDGKNV 370 380 390 400 410 420 VMLEYNCDSS GALLECCNTQ EKMARFYGVS QGTSTGSFLG AKCVTYFQRL LTNEKVCPQH 430 440 450 460 470 480 RLIHFMIDED DEERYTARCM MGFAEQAGFR TKLCVKLVNF RYRDGPPSNA APLATPCDHP 490 500 510 520 530 540 TIVDGEDEEV LMVWKTWSWD TVLHQYHSQR SSSDAVNTPT LSDILLNNNI RVLEPLWKAV 550 560 570 580 590 600 TGSKAILPFM HALAPDHEHM LAASFLPTRE IISRHYISKP VNGRAGQNIM MYDPVTSPTE 610 620 630 640 650 660 LEGAPQQDIC EALSQNASAR SLLNGSPLPL SQSVDQTNEC SPGKFFDSVL VYQQRLFLKK 670 680 690 700 710 FDGKYFPIFC GWMVGDEFGG VVVREDTSKI TKLSSMVVPA RVVRDNVPLG VSYSDEGET

References

[1]	"Cloning and characterization of the two enzymes responsible for trypanothione biosynthesis in Crithidia fasciculata." Tetaud E., Manai F., Barrett M.P., Nadeau K., Walsh C.T., Fairlamb A.H. J. Biol. Chem. 273:19383-19390(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: HS6.
[2]	"Purification of glutathionylspermidine and trypanothione synthetases from Crithidia fasciculata." Smith K., Nadeau K., Bradley M., Walsh C., Fairlamb A.H. Protein Sci. 1:874-883(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 191-209; 351-358; 512-530; 532-538 AND 695-710, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U66520 Genomic DNA. Translation: AAC48361.2.
3D structure databases
ProteinModelPortal	P90518.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	6.3.1.8. 1365.
SABIO-RK	P90518.
Family and domain databases
InterPro	IPR007921. CHAP. IPR005494. GSPS_pre-ATP-grasp-like_dom. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF05257. CHAP. 1 hit. PF03738. GSP_synth. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
PROSITE	PS50911. CHAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P90518.
ChEMBL	CHEMBL3354.

Entry information

Entry name

GSP_CRIFA

Accession

Primary (citable) accession number: P90518

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)