Reviewed, UniProtKB/Swiss-Prot P90513 (PSA3_ACACA)

Names and origin

Protein names	Recommended name:     Proteasome subunit alpha type-3     EC=3.4.25.1
Organism	Acanthamoeba castellanii (Amoeba)
Taxonomic identifier	5755 [NCBI]
Taxonomic lineage	Eukaryota › Amoebozoa › Centramoebida › Acanthamoebidae › Acanthamoeba

Protein attributes

Sequence length	252 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 252	›252	Proteasome subunit alpha type-3		PRO_0000124094
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P90513-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 71898276B50C2E79	FASTA	252	27,728
	10 20 30 40 50 60 SIGTGYDLSS TTFSPDGRVF QVEYAAKAVD NSGTALGLRV KDGVVLAVEK LLVSKMLVPN 70 80 90 100 110 120 TNRRIHTVDR HCGLAMSGLV ADGRQLVSRG RAEATSYREF YGTDISGKVL NERLSNFVQL 130 140 150 160 170 180 YSLYGSVRPF GTSVILGCVD KNGPQLYMIE PSGISWGYFG VAIGKGARAA KTEIEKLKLS 190 200 210 220 230 240 EMTAREAIKE AAKIIYSVHD DAKDKAFELE LSWVCEETGN LHKFVPKDLL EEAEKYAKQA 250 LEEEEDMSEE ED

References

[1]	Xu P., Zot H.G. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U85398 mRNA. Translation: AAB41645.1.
3D structure databases
SMR	P90513. Positions 1-243.
ModBase	Search...
Protein family/group databases
MEROPS	T01.977.
Enzyme and pathway databases
BRENDA	3.4.25.1. 3105.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA3_ACACA

Accession

Primary (citable) accession number: P90513

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)