Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidylate synthase

Gene

70

Organism
Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74dUMPBy similarity1
Active sitei219NucleophileBy similarity1
Binding sitei2425,10-methylenetetrahydrofolateBy similarity1
Binding sitei250dUMPBy similarity1
Binding sitei3365,10-methylenetetrahydrofolate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi199 – 200dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi239 – 242dUMPBy similarity4
Nucleotide bindingi280 – 282dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:70
OrganismiHuman herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Taxonomic identifieri868565 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000000942 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410651 – 337Thymidylate synthaseAdd BLAST337

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1337
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi54 – 67Combined sources14
Beta strandi69 – 72Combined sources4
Beta strandi78 – 90Combined sources13
Beta strandi92 – 94Combined sources3
Beta strandi99 – 101Combined sources3
Helixi105 – 116Combined sources12
Helixi122 – 126Combined sources5
Turni127 – 129Combined sources3
Helixi134 – 137Combined sources4
Helixi139 – 145Combined sources7
Helixi159 – 165Combined sources7
Beta strandi173 – 175Combined sources3
Helixi184 – 194Combined sources11
Beta strandi202 – 204Combined sources3
Helixi208 – 213Combined sources6
Beta strandi214 – 216Combined sources3
Beta strandi219 – 228Combined sources10
Beta strandi231 – 242Combined sources12
Turni243 – 246Combined sources4
Helixi247 – 265Combined sources19
Beta strandi268 – 282Combined sources15
Helixi283 – 285Combined sources3
Helixi286 – 292Combined sources7
Beta strandi302 – 305Combined sources4
Helixi312 – 314Combined sources3
Helixi317 – 319Combined sources3
Beta strandi320 – 323Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5H38X-ray1.70A/B51-334[»]
5H39X-ray2.00A/B51-335[»]
5H3AX-ray2.40A/B51-333[»]
ProteinModelPortaliP90463.
SMRiP90463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

KOiK00560.
OrthoDBiVOG090000GN.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PfamiView protein in Pfam
PF00303. Thymidylat_synt. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiView protein in PROSITE
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P90463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPFVPLSLY VAKKLFRARG FRFCQKPGVL ALAPEVDPCS IQHEVTGAET
60 70 80 90 100
PHEELQYLRQ LREILCRGSD RLDRTGIGTL SLFGMQARYS LRDHFPLLTT
110 120 130 140 150
KRVFWRGVVQ ELLWFLKGST DSRELSRTGV KIWDKNGSRE FLAGRGLAHR
160 170 180 190 200
REGDLGPVYG FQWRHFGAAY VDADADYTGQ GFDQLSYIVD LIKNNPHDRR
210 220 230 240 250
IIMCAWNPAD LSLMALPPCH LLCQFYVADG ELSCQLYQRS GDMGLGVPFN
260 270 280 290 300
IASYSLLTYM LAHVTGLRPG EFIHTLGDAH IYKTHIEPLR LQLTRTPRPF
310 320 330
PRLEILRSVS SMEEFTPDDF RLVDYCPHPT IRMEMAV
Length:337
Mass (Da):38,555
Last modified:May 1, 1997 - v1
Checksum:iD0EB0CE60A3384EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75698 Genomic DNA. Translation: AAC57092.1.
U83348 Genomic DNA. Translation: AAC56948.1.
U93872 Genomic DNA. Translation: AAB62673.1.
U71365 Genomic DNA. Translation: AAC34940.1.
AF148805 Genomic DNA. Translation: ABD28859.1.
RefSeqiYP_001129361.1. NC_009333.1.

Genome annotation databases

GeneIDi4961481.
KEGGivg:4961481.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTYSY_HHV8P
AccessioniPrimary (citable) accession number: P90463
Secondary accession number(s): D0UZM0, Q2HRC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: July 5, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families