P89684 (POL2_BAMMN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Genome polyprotein 2

Cleaved into the following 2 chains:

1. Helper component proteinase
   Short name=HC-pro
   EC=3.4.22.45
2. 70 kDa protein

Gene names

Name:

RNA2

Organism

Barley mild mosaic virus (strain Na1) (BaMMV) [Complete proteome]

Taxonomic identifier

103900 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Potyviridae › Bymovirus

Virus host

Hordeum vulgare (Barley) [TaxID: 4513]

Protein attributes

Sequence length	891 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
Post-translational modification	The viral RNA2 of bymoviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least two individual proteins. The HC-pro cleaves its C-terminus autocatalytically Potential.
Sequence similarities	Belongs to the bymoviruses polyprotein 2 family. Contains 1 peptidase C6 domain.

Ontologies

Keywords
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	viral capsid Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Helper component proteinase Potential		PRO_0000040560
	Chain	230 – 891	662	70 kDa protein		PRO_0000040561
Sites
	Active site	117	1	For helper component proteinase activity By similarity
	Active site	189	1	For helper component proteinase activity By similarity
	Site	229 – 230	2	Cleavage; by HC-PRO protease Potential

Sequences

	Sequence		Length	Mass (Da)
	P89684 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: A290247196822BE2	FASTA	891	97,943
	10 20 30 40 50 60 MMMNSTIRQG WQQVLKRFSI PASGDRLIIS NPTDQPIGLF GAFDTSLQTL SQVGDDPEVL 70 80 90 100 110 120 KQKIHIPTHL DIASALEASP RSFPWIFLTN SFCTFGGSIH AQNLQAFATA EFKSGFCYMN 130 140 150 160 170 180 LLVPLSFDII DAHADSFRVF VEQLPDMLGA YPSLSMVLNV MLHAATRFPE IVSSPVPTIA 190 200 210 220 230 240 FDAESLQFHV TDKRGVPGMW NILKAGRVYE LLSLAADGVG CEYMLYPVGA APQYSFWKKS 250 260 270 280 290 300 MDHFTSDRFV EFLAMQNLLA SALEQDYTTH DALDALLAAL QNAGYTNVVA RERRFPNGHD 310 320 330 340 350 360 PSTVWLNLSE APISEKLTDL KRYLLVGHRS DDTADITHNV HQYVFEVLKT MSVQFSKRTN 370 380 390 400 410 420 AYNRARFEVN HKVIWNAEYG RGPQQNAELE ALVLFLNRQS LEIENILHRT TSPVVVTNWQ 430 440 450 460 470 480 PDVPTAAPEV SEGEPTHAVA TPMTEAPAHA TPVEVVNLPS TRSYWAETLV GVLTAVLGTI 490 500 510 520 530 540 FALLTRALIR AKRLRRKPTF PWVTLDSGDE DDDHSGGGGG GPQTPGGQPP ASPAHRTHQS 550 560 570 580 590 600 RLSVQDIASD TSLLSVDLDE DTLSQYDETF QRIRRALFET SFTDILQNSA RWISALEAMA 610 620 630 640 650 660 LADGNAPYTL LAQYLNGIEE AYTSFRNTGH VSRATLSSFF ALEDSLRAAG IAFGATTPTQ 670 680 690 700 710 720 TIQNQFADSP ARRWKTRFEQ IACELGDASI KSLADLADII DTERERSDLT QFDVLAASSI 730 740 750 760 770 780 SSLCRAVRII SDTTDPDAQL ALVENATAMQ NNINAILGTN VSIPFLSATR RLLVTRRIQQ 790 800 810 820 830 840 AGAENRSGAT PETIQQLADA ELIVSEANMF NEMATSQRDI ANATQEATIR EHVLSPVNAL 850 860 870 880 890 ANVGMAAAFF RSGGMRSRAL HPAMPTMPGV SAATGRPIFQ AFRGRGHRLN R

References

[1]	"The complete nucleotide sequence and genome organization of barley mild mosaic virus (Na1 strain)." Kashiwazaki S. Arch. Virol. 141:2077-2089(1996) [PubMed: 8973524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D83409 Genomic RNA. Translation: BAA18954.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C06.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001456. Peptidase_C6. IPR001337. TMV-like_coat. [Graphical view]
Pfam	PF00851. Peptidase_C6. 1 hit. PF00721. TMV_coat. 1 hit. [Graphical view]
SUPFAM	SSF47195. TMV_coat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

POL2_BAMMN

Accession

Primary (citable) accession number: P89684

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	November 16, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program