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Protein

Replicase large subunit

Gene
N/A
Organism
Tomato mosaic virus (strain Kazakh K2) (ToMV) (TMV strain K2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Replicase large subunit: is an RNA-dependent RNA polymerase active in viral RNA replication.
Replicase small subunit: is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation (By similarity).By similarityCurated

Miscellaneous

This protein is translated as a fusion protein by episodic readthrough of a termination codon. When readthrough of the terminator codon TGA occurs between the codons for Gln-1116 and Gln-1118, this results in the addition of the RdRp region to the replicase.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi833 – 840ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Suppressor of RNA silencing, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase large subunit (EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.6.4.13)
Alternative name(s):
183 kDa protein
RNA-directed RNA polymerase
Cleaved into the following chain:
Alternative name(s):
126 kDa protein
Methyltransferase/RNA helicase
Short name:
MT/HEL
OrganismiTomato mosaic virus (strain Kazakh K2) (ToMV) (TMV strain K2)
Taxonomic identifieri138312 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageVirgaviridaeTobamovirus
Virus hostiAntirrhinum majus (Garden snapdragon) [TaxID: 4151]
Capsicum (peppers) [TaxID: 4071]
Delphinium [TaxID: 46246]
Petunia [TaxID: 4101]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Tagetes (marigolds) [TaxID: 13707]
Proteomesi
  • UP000008253 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000411921 – 1616Replicase large subunitAdd BLAST1616
ChainiPRO_00000411931 – 1116Replicase small subunitAdd BLAST1116

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 281Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST210
Domaini801 – 963(+)RNA virus helicase ATP-bindingAdd BLAST163
Domaini964 – 1116(+)RNA virus helicase C-terminalAdd BLAST153
Domaini1380 – 1493RdRp catalyticPROSITE-ProRule annotationAdd BLAST114

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 458MethyltransferaseAdd BLAST409
Regioni830 – 1085HelicaseAdd BLAST256

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG09000048.

Family and domain databases

InterProiView protein in InterPro
IPR027351. (+)RNA_virus_helicase_core_dom.
IPR002588. Alphavirus-like_MT_dom.
IPR027417. P-loop_NTPase.
IPR007094. RNA-dir_pol_PSvirus.
IPR001788. Tymovirus_RNA-dep_RNA_pol.
PfamiView protein in Pfam
PF00978. RdRP_2. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF01660. Vmethyltransf. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51743. ALPHAVIRUS_MT. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P89676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYTQTATSS ALLETVRGNN TLVNDLAKRR LYDTAVDEFN ARDRRPKVNF
60 70 80 90 100
SKVVSEEQTL IATKAYPEFQ ITFYNTQNAV HSLAGGLRSL ELEYLMMQIP
110 120 130 140 150
YGSLTYDIGG NFASHLFKGR AYVHCCMPNL DVRDIMRHEG QKDSIELYLS
160 170 180 190 200
RLERGNKHVP NFQKEAFERY AEMPNEVVCH DTFQTCRHSQ ECYTGRVYAI
210 220 230 240 250
ALHSIYDIPA DEFGAALLRK NVHVCYAAFH FSENLLLEDS HVNLDEINAC
260 270 280 290 300
FQRDGDRLTF SFASESTLNH SHSYSNILKY VCKTYFPASN REVYMKEFLV
310 320 330 340 350
TRVNTWFCKF SRIDTFLFYK GVAHKGVDSE QFYKAMEDAW HYKKTLAMCN
360 370 380 390 400
SERILLEDSS SVNYWFPKMR DMVIVPLFDI SLETSKRTRK EVLVSKDFVY
410 420 430 440 450
TVLNHIRTYQ AKALTYSNVL SFVESIRSRV IINGVTARSE WDVDKSLLQS
460 470 480 490 500
LSMTFFLHTK LAVLKDDLLI SKFALGPKTV SQHVWDEISL AFGNAFPSIK
510 520 530 540 550
ERLINRKLIK ITENALEIRV PDLYVTFHDR LVSEYKMSVD MPVLDIRKKM
560 570 580 590 600
EETEEMYNAL SELSVLKNSD KFDVDVFSQM CQSLEVDPMT AAKVIVAVMS
610 620 630 640 650
NESGLTLTFE QPTEANVALA LQDSEKASDG ALVVTSRDVE EPSIRGSMAR
660 670 680 690 700
GELQLAGLSG DVPESSYTRS EEIESLEQFH MATASSLIHK QMCSIVYTGP
710 720 730 740 750
LKVQQMKNFI DSLVASLSAA VSNLVKILKD TAAIDLETRQ KFGVLDVASK
760 770 780 790 800
RWLVKPSAKN HAWGVVETHA RKYHVALLEH DEFGIITCDN WRRVAVSSES
810 820 830 840 850
VVYSDMAKLR TLRRLLKDGE PHVSSAKVVL VDGVPGCGKT KEILSRVNFE
860 870 880 890 900
EDLILVPGRQ AAEMIRRRAN ASGIIVATKD NVRTVDSFLM NYGKGARCQF
910 920 930 940 950
KRLFIDEGLM LHTGCVNFLV EMSLCDIAYV YGDTQQIPYI NRVTGFPYPA
960 970 980 990 1000
HFAKLEVDEV ETRRTTLRCP ADVTHFLNQR YEGHVMCTSS EKKSVSQEMV
1010 1020 1030 1040 1050
SGAASINPVS KPLKGKILTF TQSDKEALLS RGYADVHTVH EVQGETYADV
1060 1070 1080 1090 1100
SLVRLTPTPV SIIARDSPHV LVSLSRHTKS LKYYTVVMDP LVSIIRDLER
1110 1120 1130 1140 1150
VSSYLLDMYK VDAGTQXQLQ VDSVFKNFNL FVAAPKTGDI SDMQFYYDKC
1160 1170 1180 1190 1200
LPGNSTLLNN YDAVTMKLTD ISLNVKDCIL DMSKSVAAPK DVKPTLIPMV
1210 1220 1230 1240 1250
RTAAEMPRQT GLLENLVAMI KRNFNSPELS GVVDIENTAS LVVDKFFDSY
1260 1270 1280 1290 1300
LLKEKRKPNK NFSLFSRESL NRWIAKQEQV TIGQLADFDF VDLPAVDQYR
1310 1320 1330 1340 1350
HMIKAQPKQK LDLSIQTEYP ALQTIVYHSK KINAIFGPLF SELTRQLLDS
1360 1370 1380 1390 1400
IDSSRFLFFT RKTPAQIEDF FGDLDSHVPM DVLELDVSKY DKSQNEFHCA
1410 1420 1430 1440 1450
VEYEIWRRLG LEDFLAEVWK QGHRKTTLKD YTAGIKTCLW YQRKSGDVTT
1460 1470 1480 1490 1500
FIGNTVIIAS CLASMLPMEK LIKGAFCGDD SLLYFPKGCE YPDIQQAANL
1510 1520 1530 1540 1550
MWNFEAKLFK KQYGYFCGRY VIHHDRGCIV YYDPLKLISK LGAKHIKDWD
1560 1570 1580 1590 1600
HLEEFRRSLC DVAESLNNCA YYTQLDDAVG EVHKTAPPGS FVYKSLVKYL
1610
SDKVLFRSLF LDGSSC
Length:1,616
Mass (Da):183,617
Last modified:May 1, 1997 - v1
Checksum:iC2F5CE0C8C965336
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z92909 Genomic RNA. Translation: CAB07439.1.
Z92909 Genomic RNA. Translation: CAB07438.1.

Keywords - Coding sequence diversityi

RNA suppression of termination

Similar proteinsi

Entry informationi

Entry nameiRDRP_TOMK2
AccessioniPrimary (citable) accession number: P89676
Secondary accession number(s): P90349
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: April 12, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families