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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RIR1

Organism
Human herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity). The N-terminal region confers antiapoptotic activity in differentiated cells such as neurons and is important for viral reactivation to increase neural survivability.UniRule annotation1 Publication

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei571SubstrateUniRule annotation1
Sitei587Important for hydrogen atom transferUniRule annotation1
Binding sitei617Substrate; via amide nitrogenUniRule annotation1
Active sitei796Proton acceptorUniRule annotation1
Active sitei798Cysteine radical intermediateUniRule annotation1
Active sitei800Proton acceptorUniRule annotation1
Sitei813Important for hydrogen atom transferUniRule annotation1
Sitei1116Important for electron transferUniRule annotation1
Sitei1117Important for electron transferUniRule annotation1
Sitei1137Interacts with thioredoxin/glutaredoxinUniRule annotation1
Sitei1140Interacts with thioredoxin/glutaredoxinUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-UniPathway
  • negative regulation of tumor necrosis factor-mediated signaling pathway Source: AgBase
  • viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication, Host-virus interaction

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:RIR1UniRule annotation
Ordered Locus Names:UL39
OrganismiHuman herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Taxonomic identifieri10315 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000001874 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host endosome, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003851621 – 1142Ribonucleoside-diphosphate reductase large subunitAdd BLAST1142

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi587 ↔ 813Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Protein-protein interaction databases

BioGridi1677930. 1 interactor.
IntActiP89462. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP89462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni294 – 400alpha-crystallin domainAdd BLAST107
Regioni586 – 587Substrate bindingUniRule annotation2
Regioni796 – 800Substrate bindingUniRule annotation5
Regioni973 – 977Substrate bindingUniRule annotation5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi151 – 159Poly-Pro9
Compositional biasi186 – 239Asp/Ser-richAdd BLAST54

Domaini

Contains an alpha-crystallin domain homologous to small heat-shock proteins.

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Phylogenomic databases

KOiK19448.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P89462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANRPAASAL AGARSPSERQ EPREPEVAPP GGDHVFCRKV SGVMVLSSDP
60 70 80 90 100
PGPAAYRISD SSFVQCGSNC SMIIDGDVAR GHLRDLEGAT STGAFVAISN
110 120 130 140 150
VAAGGDGRTA VVALGGTSGP SATTSVGTQT SGEFLHGNPR TPEPQGPQAV
160 170 180 190 200
PPPPPPPFPW GHECCARRDA RGGAEKDVGA AESWSDGPSS DSETEDSDSS
210 220 230 240 250
DEDTGSETLS RSSSIWAAGA TDDDDSDSDS RSDDSVQPDV VVRRRWSDGP
260 270 280 290 300
APVAFPKPRR PGDSPGNPGL GAGTGPGSAT DPRASADSDS AAHAAAPQAD
310 320 330 340 350
VAPVLDSQPT VGTDPGYPVP LELTPENAEA VARFLGDAVD REPALMLEYF
360 370 380 390 400
CRCAREESKR VPPRTFGSAP RLTEDDFGLL NYALAEMRRL CLDLPPVPPN
410 420 430 440 450
AYTPYHLREY ATRLVNGFKP LVRRSARLYR ILGVLVHLRI RTREASFEEW
460 470 480 490 500
MRSKEVDLDF GLTERLREHE AQLMILAQAL NPYDCLIHST PNTLVERGLQ
510 520 530 540 550
SALKYEEFYL KRFGGHYMES VFQMYTRIAG FLACRATRGM RHIALGRQGS
560 570 580 590 600
WWEMFKFFFH RLYDHQIVPS TPAMLNLGTR NYYTSSCYLV NPQATTNQAT
610 620 630 640 650
LRAITGNVSA ILARNGGIGL CMQAFNDASP GTASIMPALK VLDSLVAAHN
660 670 680 690 700
KQSTRPTGAC VYLEPWHSDV RAVLRMKGVL AGEEAQRCDN IFSALWMPDL
710 720 730 740 750
FFKRLIRHLD GEKNVTWSLF DRDTSMSLAD FHGEEFEKLY EHLEAMGFGE
760 770 780 790 800
TIPIQDLAYA IVRSAATTGS PFIMFKDAVN RHYIYDTQGA AIAGSNLCTE
810 820 830 840 850
IVHPASKRSS GVCNLGSVNL ARCVSRQTFD FGRLRDAVQA CVLMVNIMID
860 870 880 890 900
STLQPTPQCT RGNDNLRSMG IGMQGLHTAC LKMGLDLESA EFRDLNTHIA
910 920 930 940 950
EVMLLAAMKT SNALCVRGAR PFSHFKRSMY RAGRFHWERF SNASPRYEGE
960 970 980 990 1000
WEMLRQSMMK HGLRNSQFIA LMPTAASAQI SDVSEGFAPL FTNLFSKVTR
1010 1020 1030 1040 1050
DGETLRPNTL LLKELERTFG GKRLLDAMDG LEAKQWSVAQ ALPCLDPAHP
1060 1070 1080 1090 1100
LRRFKTAFDY DQELLIDLCA DRAPYVDHSQ SMTLYVTEKA DGTLPASTLV
1110 1120 1130 1140
RLLVHAYKRG LKTGMYYCKV RKATNSGVFA GDDNIVCTSC AL
Length:1,142
Mass (Da):124,923
Last modified:May 1, 1997 - v1
Checksum:i5A46D3985338BB21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z86099 Genomic DNA. Translation: CAB06725.1.
PIRiA05247.
RefSeqiYP_009137191.1. NC_001798.2.

Genome annotation databases

GeneIDi1487325.
KEGGivg:1487325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z86099 Genomic DNA. Translation: CAB06725.1.
PIRiA05247.
RefSeqiYP_009137191.1. NC_001798.2.

3D structure databases

ProteinModelPortaliP89462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1677930. 1 interactor.
IntActiP89462. 2 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1487325.
KEGGivg:1487325.

Phylogenomic databases

KOiK19448.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_HHV2H
AccessioniPrimary (citable) accession number: P89462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.