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P89459 (DEN_HHV2H) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

Short name=pUL36
EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Tegument protein VP1-2
Tegument protein VP1/2
Ubiquitin thioesterase pUL36
Gene names
ORF Names:UL36
OrganismHuman herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2) [Reference proteome]
Taxonomic identifier10315 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length3122 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with UL37 By similarity.

Subcellular location

Virion tegument By similarity. Host cytoplasm By similarity. Host nucleus By similarity. Note: Tightly associated with the capsid By similarity.

Post-translational modification

Proteolytically processed, possibly into several polypeptides. Enzymatic activity is only detectable following cleavage of the UL36 protein, which occurs late during viral replication By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 31223122Deneddylase
PRO_0000385478

Regions

Domain20 – 238219Peptidase C76
Repeat2891 – 289551
Repeat2896 – 290052
Repeat2901 – 290553
Repeat2906 – 291054
Repeat2911 – 291555
Repeat2916 – 292056
Repeat2921 – 292557
Repeat2926 – 293058
Repeat2931 – 293559
Repeat2936 – 2940510
Repeat2941 – 2945511
Region2891 – 29455511 X 5 AA tandem repeats of P-Q-P-P-L
Coiled coil709 – 73931 Potential
Coiled coil963 – 102260 Potential
Coiled coil1287 – 135468 Potential
Coiled coil1586 – 161631 Potential
Motif394 – 4018Nuclear localization signal By similarity
Compositional bias284 – 494211Pro-rich
Compositional bias850 – 8545Poly-Pro
Compositional bias1519 – 15224Poly-Ala
Compositional bias1848 – 18514Poly-Ala
Compositional bias2525 – 2966442Pro-rich

Sites

Active site401 By similarity
Active site1741 Potential

Sequences

Sequence LengthMass (Da)Tools
P89459 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 6EBF94B51BFE8C0B

FASTA3,122330,051
        10         20         30         40         50         60 
MIPAALPHPT MKRQGDRDIV VTGVRNQFAT DLEPGGSVSC MRSSLSFLSL LFDVGPRDVL 

        70         80         90        100        110        120 
SAEAIEGCLV EGGEWTRAAA GSGPPRMCSI IELPNFLEYP AARGGLRCVF SRVYGEVGFF 

       130        140        150        160        170        180 
GEPTAGLLET QCPAHTFFAG PWAMRPLSYT LLTIGPLGMG LYRDGDTAYL FDPHGLPAGT 

       190        200        210        220        230        240 
PAFIAKVRAG DVYPYLTYYA HDRPKVRWAG AMVFFVPSGP GAVAPADLTA AALHLYGASE 

       250        260        270        280        290        300 
TYLQDEPFVE RRVAITHPLR GEIGGLGALF VGVVPRGDGE GSGPVVPALP APTHVQTPGA 

       310        320        330        340        350        360 
DRPPEAPRGA SGPPDTPQAG HPNRPPDDVW AAALEGTPPA KPSAPDAAAS GPPHAAPPPQ 

       370        380        390        400        410        420 
TPAGDAAEEA EDLRVLEVGA VPVGRHRARY STGLPKRRRP TWTPPSSVED LTSGERPAPK 

       430        440        450        460        470        480 
APPAKAKKKS APKKKAPVAA EVPASSPTPI AATVPPAPDT PPQSGQGGGD DGPASPSSPS 

       490        500        510        520        530        540 
VLETLGARRP PEPPGADLAQ LFEVHPNVAA TAVRLAARDA ALAREVAACS QLTINALRSP 

       550        560        570        580        590        600 
YPAHPGLLEL CVIFFFERVL AFLIENGART HTQAGVAGPA AALLDFTLRM LPRKTAVGDF 

       610        620        630        640        650        660 
LASTRMSLAD VAAHRPLIQH VLDENSQIGR LALAKLVLVA RDVIRETDAF YGDLADLDLQ 

       670        680        690        700        710        720 
LRAAPPANLY ARLGEWLLER SRAHPNTLFA PATPTHPEPL LHRIQALAQF ARGEEMRVEA 

       730        740        750        760        770        780 
EAREMREALD ALARGVDSVS QRAGPLTVMP VPAAPGAGGR APCPPALGPE AIQARLEDVR 

       790        800        810        820        830        840 
IQARRAIESA VKEYFHRGAV YSAKALQASD SHDCRFHVAS AAVVPMVQLL ESLPAFDQHT 

       850        860        870        880        890        900 
RDVAQRAALP PPPPLATSPQ AILLRDLLQR GQPLDAPEDL AAWLSVLTDA ATQGLIERKP 

       910        920        930        940        950        960 
LEELARSIHG INDQQARRSS GLAELQRFDA LDAALAQQLD SDAAFVPATG PAPYVDGGGL 

       970        980        990       1000       1010       1020 
SPEATRMAED ALRQARAMEA AKMTAELAPE ARSRLRERAH ALEAMLNDAR ERAKVAHDAR 

      1030       1040       1050       1060       1070       1080 
EKFLHKLQGV LRPLPDFVGL KACPAVLATL RASLPAGWTD LADAVRGPPP EVTAALRADL 

      1090       1100       1110       1120       1130       1140 
WGLLGQYREA LEHPTPDTAT ALAGLHPAFV VVLKTLFADA PETPVLVQFF SDHAPTIAKA 

      1150       1160       1170       1180       1190       1200 
VSNAINAGSA AVATASPAAT VDAAVRAHGA LADAVSALGA AARDPASPLS FLAVLADSAA 

      1210       1220       1230       1240       1250       1260 
GYVKATRLAL EARGAIDELT TLGSAAADLV VQARRACAQP EGDHAALIDA AARATTAARE 

      1270       1280       1290       1300       1310       1320 
SLAGHEAGFG GLLHAEGTAG DHSPSGRALQ ELGKVIGATR RRADELEAAV ADLTAKMAAQ 

      1330       1340       1350       1360       1370       1380 
RARGSSERWA AGVEAALDRV ENRAEFDVVE LRRLQALAGT HGYNPRDFRK RAEQALAANA 

      1390       1400       1410       1420       1430       1440 
EAVTLALDTA FAFNPYTPEN QRHPMLPPLA AIHRLGWSAA FHAAAETYAD MFRVDAEPLA 

      1450       1460       1470       1480       1490       1500 
RLLRIAEGLL EMAQAGDGFI DYHEAVGRLA DDMTSVPGLR RYVPFFQHGY ADYVELRDRL 

      1510       1520       1530       1540       1550       1560 
DAIRADVHRA LGGVPLDLAA AAEQISAARN DPEATAELVR TGVTLPCPSE DALVACAAAL 

      1570       1580       1590       1600       1610       1620 
ERVDQSPVKN TAYAEYVAFV TRQDTAETKD AVVRAKQQRA EATERVMAGL REALAARERR 

      1630       1640       1650       1660       1670       1680 
AQIEAEGLAN LKTMLKVVAV PATVAKTLDQ ARSVAEIADQ VEVLLDQTEK TRELDVPAVI 

      1690       1700       1710       1720       1730       1740 
WLEHAQRTFE THPLSAARGD GPGPLARHAG RLGALFDTRR RVDALRRSLE EAEAEWDEVW 

      1750       1760       1770       1780       1790       1800 
GRFGRVRGGA WKSPEGFRAM HEQLRALQDT TNTVSGLRAQ PAYERLSARY QGVLGAKGAE 

      1810       1820       1830       1840       1850       1860 
RAEAVEELGA RVTKHTALCA RLRDEVVRRV PWEMNFDALG GLLAEFDAAA ADLAPWAVEE 

      1870       1880       1890       1900       1910       1920 
FRGARELIQY RMGLYSAYAR AGGQTGAGAE SAPAPLLVDL RALDARARAS SSPEGHEVDP 

      1930       1940       1950       1960       1970       1980 
QLLRRRGEAY LRAGGDPGPL VLREAVSALD LPFATSFLAP DGTPLQYALC FPAVTDKLGA 

      1990       2000       2010       2020       2030       2040 
LLMRPEAACV RPPLPTDVLE SAPTVTAMYV LTVVNRLQLA LSDAQAANFQ LFGRFVRHRQ 

      2050       2060       2070       2080       2090       2100 
ATWGASMDAA AELYVALVAT TLTREFGCRW AQLGWASGAA APRPPPGPRG SQRHCVAFNE 

      2110       2120       2130       2140       2150       2160 
NDVLVALVAG VPEHIYNFWR LDLVRQHEYM HLTLERAFED AAESMLFVQR LTPHPDARIR 

      2170       2180       2190       2200       2210       2220 
VLPTFLDGGP PTRGLLFGTR LADWRRGKLS ETDPLAPWRS ALELGTQRRD VPALGKLSPA 

      2230       2240       2250       2260       2270       2280 
QALAAVSVLG RMCLPSAALA ALWTCMFPDD YTEYDSFDAL LAARLESGQT LGPAGGREAS 

      2290       2300       2310       2320       2330       2340 
LPEAPHALYR PTGQHVAVLA AATHRTPAAR VTAMDLVLAA VLLGAPVVVA LRNTTAFSRE 

      2350       2360       2370       2380       2390       2400 
SELELCLTLF DSRPGGPDAA LRDVVSSDIE TWAVGLLHTD LNPIENACLA AQLPRLSALI 

      2410       2420       2430       2440       2450       2460 
AERPLADGPP CLVLVDISMT PVAVLWEAPE PPGPPDVRFV GSEATEELPF VATAGDVLAA 

      2470       2480       2490       2500       2510       2520 
SAADADPFFA RAILGRPFDA SLLTGELFPG HPVYQRPLAD EAGPSAPTAA RDPRDLAGGD 

      2530       2540       2550       2560       2570       2580 
GGSGPEDPAA PPARQADPGV LAPTLLTDAT TGEPVPPRMW AWIHGLEELA SDDAGGPTPN 

      2590       2600       2610       2620       2630       2640 
PAPALLPPPA TDQSVPTSQY APRPIGPAAT ARETRPSVPP QQNTGRVPVA PRDDPRPSPP 

      2650       2660       2670       2680       2690       2700 
TPSPPADAAL PPPAFSGSAA AFSAAVPRVR RSRRTRAKSR APRASAPPEG WRPPALPAPV 

      2710       2720       2730       2740       2750       2760 
APVAASARPP DQPPTPESAP PAWVSALPLP PGPASARGAF PAPTLAPIPP PPAEGAVVPG 

      2770       2780       2790       2800       2810       2820 
GDRRRGRRQT TAGPSPTPPR GPAAGPPRRL TRPAVASLSA SLNSLPSPRD PADHAAAVSA 

      2830       2840       2850       2860       2870       2880 
AAAAVPPSPG LAPPTSAVQT SPPPLAPGPV APSEPLCGWV VPGGPVARRP PPQSPATKPA 

      2890       2900       2910       2920       2930       2940 
ARTRIRARSV PQPPLPQPPL PQPPLPQPPL PQPPLPQPPL PQPPLPQPPL PQPPLPQPPL 

      2950       2960       2970       2980       2990       3000 
PQPPLPPVTR TLTPQSRDSV PTPESPTHTN THLPVSAVTS WASSLALHVD SAPPPASLLQ 

      3010       3020       3030       3040       3050       3060 
TLHISSDDEH SDADSLRFSD SDDTEALDPL PPEPHLPPAD EPPGPLAADH LQSPHSQFGP 

      3070       3080       3090       3100       3110       3120 
LPVQANAVLS RRYVRSTGRS ALAVLIRACR RIQQQLQRTR RALFQRSNAV LTSLHHVRML 


LG 

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References

[1]"The genome sequence of herpes simplex virus type 2."
Dolan A., Jamieson F.E., Cunningham C., Barnett B.C., McGeoch D.J.
J. Virol. 72:2010-2021(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z86099 Genomic DNA. Translation: CAB06722.1.
RefSeqNP_044506.1. NC_001798.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC76.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1487322.

Family and domain databases

InterProIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_HHV2H
AccessionPrimary (citable) accession number: P89459
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 1997
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries