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Protein

Large tegument protein deneddylase

Gene

UL36

Organism
Human herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Large tegument protein that plays multiple roles in the viral cycle. During viral entry, remains associated with the capsid while most of the tegument is detached and participates in the capsid transport toward the host nucleus. Plays a role in the routing of the capsid at the nuclear pore complex and subsequent uncoating. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favorable environment allowing efficient viral genome replication. Participates later in the secondary envelopment of capsids. Indeed, plays a linker role for the association of the outer viral tegument to the capsids together with the inner tegument protein.UniRule annotation

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei27Important for catalytic activityUniRule annotation1
Active sitei40UniRule annotation1
Active sitei172UniRule annotation1
Active sitei174UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Protein family/group databases

MEROPSiC76.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Large tegument protein deneddylaseUniRule annotation (EC:3.4.19.12UniRule annotation, EC:3.4.22.-UniRule annotation)
Gene namesi
ORF Names:UL36
OrganismiHuman herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Taxonomic identifieri10315 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000001874 Componenti: Genome

Subcellular locationi

  • Virion tegument UniRule annotation
  • Host cytoplasm UniRule annotation
  • Host nucleus UniRule annotation

  • Note: Tightly associated with the capsid.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003854781 – 3122Large tegument protein deneddylaseAdd BLAST3122

Post-translational modificationi

Proteolytically processed, possibly into several polypeptides. Enzymatic activity is only detectable following cleavage of the UL36 protein, which occurs late during viral replication (By similarity).By similarity

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with inner tegument protein. Interacts with capsid vertex specific component CVC2. Interacts with the major capsid protein/MCP.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 238Peptidase C76UniRule annotationAdd BLAST219
Repeati2891 – 289515
Repeati2896 – 290025
Repeati2901 – 290535
Repeati2906 – 291045
Repeati2911 – 291555
Repeati2916 – 292065
Repeati2921 – 292575
Repeati2926 – 293085
Repeati2931 – 293595
Repeati2936 – 2940105
Repeati2941 – 2945115

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 248Deubiquitination activityUniRule annotationAdd BLAST248
Regioni548 – 578Interaction with inner tegument proteinUniRule annotationAdd BLAST31
Regioni2891 – 294511 X 5 AA tandem repeats of P-Q-P-P-LAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi284 – 494Pro-richAdd BLAST211
Compositional biasi850 – 854Poly-Pro5
Compositional biasi1519 – 1522Poly-Ala4
Compositional biasi1848 – 1851Poly-Ala4
Compositional biasi2525 – 2966Pro-richAdd BLAST442

Sequence similaritiesi

Belongs to the herpesviridae large tegument protein family.UniRule annotation
Contains 1 peptidase C76 domain.UniRule annotation

Keywords - Domaini

Repeat

Family and domain databases

HAMAPiMF_04044. HSV_LTP. 1 hit.
InterProiIPR005210. Herpes_LT_deneddylase.
IPR006928. htUSP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P89459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPAALPHPT MKRQGDRDIV VTGVRNQFAT DLEPGGSVSC MRSSLSFLSL
60 70 80 90 100
LFDVGPRDVL SAEAIEGCLV EGGEWTRAAA GSGPPRMCSI IELPNFLEYP
110 120 130 140 150
AARGGLRCVF SRVYGEVGFF GEPTAGLLET QCPAHTFFAG PWAMRPLSYT
160 170 180 190 200
LLTIGPLGMG LYRDGDTAYL FDPHGLPAGT PAFIAKVRAG DVYPYLTYYA
210 220 230 240 250
HDRPKVRWAG AMVFFVPSGP GAVAPADLTA AALHLYGASE TYLQDEPFVE
260 270 280 290 300
RRVAITHPLR GEIGGLGALF VGVVPRGDGE GSGPVVPALP APTHVQTPGA
310 320 330 340 350
DRPPEAPRGA SGPPDTPQAG HPNRPPDDVW AAALEGTPPA KPSAPDAAAS
360 370 380 390 400
GPPHAAPPPQ TPAGDAAEEA EDLRVLEVGA VPVGRHRARY STGLPKRRRP
410 420 430 440 450
TWTPPSSVED LTSGERPAPK APPAKAKKKS APKKKAPVAA EVPASSPTPI
460 470 480 490 500
AATVPPAPDT PPQSGQGGGD DGPASPSSPS VLETLGARRP PEPPGADLAQ
510 520 530 540 550
LFEVHPNVAA TAVRLAARDA ALAREVAACS QLTINALRSP YPAHPGLLEL
560 570 580 590 600
CVIFFFERVL AFLIENGART HTQAGVAGPA AALLDFTLRM LPRKTAVGDF
610 620 630 640 650
LASTRMSLAD VAAHRPLIQH VLDENSQIGR LALAKLVLVA RDVIRETDAF
660 670 680 690 700
YGDLADLDLQ LRAAPPANLY ARLGEWLLER SRAHPNTLFA PATPTHPEPL
710 720 730 740 750
LHRIQALAQF ARGEEMRVEA EAREMREALD ALARGVDSVS QRAGPLTVMP
760 770 780 790 800
VPAAPGAGGR APCPPALGPE AIQARLEDVR IQARRAIESA VKEYFHRGAV
810 820 830 840 850
YSAKALQASD SHDCRFHVAS AAVVPMVQLL ESLPAFDQHT RDVAQRAALP
860 870 880 890 900
PPPPLATSPQ AILLRDLLQR GQPLDAPEDL AAWLSVLTDA ATQGLIERKP
910 920 930 940 950
LEELARSIHG INDQQARRSS GLAELQRFDA LDAALAQQLD SDAAFVPATG
960 970 980 990 1000
PAPYVDGGGL SPEATRMAED ALRQARAMEA AKMTAELAPE ARSRLRERAH
1010 1020 1030 1040 1050
ALEAMLNDAR ERAKVAHDAR EKFLHKLQGV LRPLPDFVGL KACPAVLATL
1060 1070 1080 1090 1100
RASLPAGWTD LADAVRGPPP EVTAALRADL WGLLGQYREA LEHPTPDTAT
1110 1120 1130 1140 1150
ALAGLHPAFV VVLKTLFADA PETPVLVQFF SDHAPTIAKA VSNAINAGSA
1160 1170 1180 1190 1200
AVATASPAAT VDAAVRAHGA LADAVSALGA AARDPASPLS FLAVLADSAA
1210 1220 1230 1240 1250
GYVKATRLAL EARGAIDELT TLGSAAADLV VQARRACAQP EGDHAALIDA
1260 1270 1280 1290 1300
AARATTAARE SLAGHEAGFG GLLHAEGTAG DHSPSGRALQ ELGKVIGATR
1310 1320 1330 1340 1350
RRADELEAAV ADLTAKMAAQ RARGSSERWA AGVEAALDRV ENRAEFDVVE
1360 1370 1380 1390 1400
LRRLQALAGT HGYNPRDFRK RAEQALAANA EAVTLALDTA FAFNPYTPEN
1410 1420 1430 1440 1450
QRHPMLPPLA AIHRLGWSAA FHAAAETYAD MFRVDAEPLA RLLRIAEGLL
1460 1470 1480 1490 1500
EMAQAGDGFI DYHEAVGRLA DDMTSVPGLR RYVPFFQHGY ADYVELRDRL
1510 1520 1530 1540 1550
DAIRADVHRA LGGVPLDLAA AAEQISAARN DPEATAELVR TGVTLPCPSE
1560 1570 1580 1590 1600
DALVACAAAL ERVDQSPVKN TAYAEYVAFV TRQDTAETKD AVVRAKQQRA
1610 1620 1630 1640 1650
EATERVMAGL REALAARERR AQIEAEGLAN LKTMLKVVAV PATVAKTLDQ
1660 1670 1680 1690 1700
ARSVAEIADQ VEVLLDQTEK TRELDVPAVI WLEHAQRTFE THPLSAARGD
1710 1720 1730 1740 1750
GPGPLARHAG RLGALFDTRR RVDALRRSLE EAEAEWDEVW GRFGRVRGGA
1760 1770 1780 1790 1800
WKSPEGFRAM HEQLRALQDT TNTVSGLRAQ PAYERLSARY QGVLGAKGAE
1810 1820 1830 1840 1850
RAEAVEELGA RVTKHTALCA RLRDEVVRRV PWEMNFDALG GLLAEFDAAA
1860 1870 1880 1890 1900
ADLAPWAVEE FRGARELIQY RMGLYSAYAR AGGQTGAGAE SAPAPLLVDL
1910 1920 1930 1940 1950
RALDARARAS SSPEGHEVDP QLLRRRGEAY LRAGGDPGPL VLREAVSALD
1960 1970 1980 1990 2000
LPFATSFLAP DGTPLQYALC FPAVTDKLGA LLMRPEAACV RPPLPTDVLE
2010 2020 2030 2040 2050
SAPTVTAMYV LTVVNRLQLA LSDAQAANFQ LFGRFVRHRQ ATWGASMDAA
2060 2070 2080 2090 2100
AELYVALVAT TLTREFGCRW AQLGWASGAA APRPPPGPRG SQRHCVAFNE
2110 2120 2130 2140 2150
NDVLVALVAG VPEHIYNFWR LDLVRQHEYM HLTLERAFED AAESMLFVQR
2160 2170 2180 2190 2200
LTPHPDARIR VLPTFLDGGP PTRGLLFGTR LADWRRGKLS ETDPLAPWRS
2210 2220 2230 2240 2250
ALELGTQRRD VPALGKLSPA QALAAVSVLG RMCLPSAALA ALWTCMFPDD
2260 2270 2280 2290 2300
YTEYDSFDAL LAARLESGQT LGPAGGREAS LPEAPHALYR PTGQHVAVLA
2310 2320 2330 2340 2350
AATHRTPAAR VTAMDLVLAA VLLGAPVVVA LRNTTAFSRE SELELCLTLF
2360 2370 2380 2390 2400
DSRPGGPDAA LRDVVSSDIE TWAVGLLHTD LNPIENACLA AQLPRLSALI
2410 2420 2430 2440 2450
AERPLADGPP CLVLVDISMT PVAVLWEAPE PPGPPDVRFV GSEATEELPF
2460 2470 2480 2490 2500
VATAGDVLAA SAADADPFFA RAILGRPFDA SLLTGELFPG HPVYQRPLAD
2510 2520 2530 2540 2550
EAGPSAPTAA RDPRDLAGGD GGSGPEDPAA PPARQADPGV LAPTLLTDAT
2560 2570 2580 2590 2600
TGEPVPPRMW AWIHGLEELA SDDAGGPTPN PAPALLPPPA TDQSVPTSQY
2610 2620 2630 2640 2650
APRPIGPAAT ARETRPSVPP QQNTGRVPVA PRDDPRPSPP TPSPPADAAL
2660 2670 2680 2690 2700
PPPAFSGSAA AFSAAVPRVR RSRRTRAKSR APRASAPPEG WRPPALPAPV
2710 2720 2730 2740 2750
APVAASARPP DQPPTPESAP PAWVSALPLP PGPASARGAF PAPTLAPIPP
2760 2770 2780 2790 2800
PPAEGAVVPG GDRRRGRRQT TAGPSPTPPR GPAAGPPRRL TRPAVASLSA
2810 2820 2830 2840 2850
SLNSLPSPRD PADHAAAVSA AAAAVPPSPG LAPPTSAVQT SPPPLAPGPV
2860 2870 2880 2890 2900
APSEPLCGWV VPGGPVARRP PPQSPATKPA ARTRIRARSV PQPPLPQPPL
2910 2920 2930 2940 2950
PQPPLPQPPL PQPPLPQPPL PQPPLPQPPL PQPPLPQPPL PQPPLPPVTR
2960 2970 2980 2990 3000
TLTPQSRDSV PTPESPTHTN THLPVSAVTS WASSLALHVD SAPPPASLLQ
3010 3020 3030 3040 3050
TLHISSDDEH SDADSLRFSD SDDTEALDPL PPEPHLPPAD EPPGPLAADH
3060 3070 3080 3090 3100
LQSPHSQFGP LPVQANAVLS RRYVRSTGRS ALAVLIRACR RIQQQLQRTR
3110 3120
RALFQRSNAV LTSLHHVRML LG
Length:3,122
Mass (Da):330,051
Last modified:May 1, 1997 - v1
Checksum:i6EBF94B51BFE8C0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z86099 Genomic DNA. Translation: CAB06722.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z86099 Genomic DNA. Translation: CAB06722.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC76.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

HAMAPiMF_04044. HSV_LTP. 1 hit.
InterProiIPR005210. Herpes_LT_deneddylase.
IPR006928. htUSP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLTP_HHV2H
AccessioniPrimary (citable) accession number: P89459
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.