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P89459

- DEN_HHV2H

UniProt

P89459 - DEN_HHV2H

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Protein

Deneddylase

Gene
UL36
Organism
Human herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401 By similarity
Active sitei174 – 1741 Reviewed prediction

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Protein family/group databases

MEROPSiC76.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Short name:
pUL36
Alternative name(s):
Tegument protein VP1-2
Tegument protein VP1/2
Ubiquitin thioesterase pUL36
Gene namesi
ORF Names:UL36
OrganismiHuman herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Taxonomic identifieri10315 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001874: Genome

Subcellular locationi

Virion tegument By similarity. Host cytoplasm By similarity. Host nucleus By similarity
Note: Tightly associated with the capsid By similarity.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31223122DeneddylasePRO_0000385478Add
BLAST

Post-translational modificationi

Proteolytically processed, possibly into several polypeptides. Enzymatic activity is only detectable following cleavage of the UL36 protein, which occurs late during viral replication By similarity.

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with UL37 By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 238219Peptidase C76Add
BLAST
Repeati2891 – 289551
Repeati2896 – 290052
Repeati2901 – 290553
Repeati2906 – 291054
Repeati2911 – 291555
Repeati2916 – 292056
Repeati2921 – 292557
Repeati2926 – 293058
Repeati2931 – 293559
Repeati2936 – 2940510
Repeati2941 – 2945511

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2891 – 29455511 X 5 AA tandem repeats of P-Q-P-P-LAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili709 – 73931 Reviewed predictionAdd
BLAST
Coiled coili963 – 102260 Reviewed predictionAdd
BLAST
Coiled coili1287 – 135468 Reviewed predictionAdd
BLAST
Coiled coili1586 – 161631 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi394 – 4018Nuclear localization signal By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi284 – 494211Pro-richAdd
BLAST
Compositional biasi850 – 8545Poly-Pro
Compositional biasi1519 – 15224Poly-Ala
Compositional biasi1848 – 18514Poly-Ala
Compositional biasi2525 – 2966442Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P89459-1 [UniParc]FASTAAdd to Basket

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MIPAALPHPT MKRQGDRDIV VTGVRNQFAT DLEPGGSVSC MRSSLSFLSL     50
LFDVGPRDVL SAEAIEGCLV EGGEWTRAAA GSGPPRMCSI IELPNFLEYP 100
AARGGLRCVF SRVYGEVGFF GEPTAGLLET QCPAHTFFAG PWAMRPLSYT 150
LLTIGPLGMG LYRDGDTAYL FDPHGLPAGT PAFIAKVRAG DVYPYLTYYA 200
HDRPKVRWAG AMVFFVPSGP GAVAPADLTA AALHLYGASE TYLQDEPFVE 250
RRVAITHPLR GEIGGLGALF VGVVPRGDGE GSGPVVPALP APTHVQTPGA 300
DRPPEAPRGA SGPPDTPQAG HPNRPPDDVW AAALEGTPPA KPSAPDAAAS 350
GPPHAAPPPQ TPAGDAAEEA EDLRVLEVGA VPVGRHRARY STGLPKRRRP 400
TWTPPSSVED LTSGERPAPK APPAKAKKKS APKKKAPVAA EVPASSPTPI 450
AATVPPAPDT PPQSGQGGGD DGPASPSSPS VLETLGARRP PEPPGADLAQ 500
LFEVHPNVAA TAVRLAARDA ALAREVAACS QLTINALRSP YPAHPGLLEL 550
CVIFFFERVL AFLIENGART HTQAGVAGPA AALLDFTLRM LPRKTAVGDF 600
LASTRMSLAD VAAHRPLIQH VLDENSQIGR LALAKLVLVA RDVIRETDAF 650
YGDLADLDLQ LRAAPPANLY ARLGEWLLER SRAHPNTLFA PATPTHPEPL 700
LHRIQALAQF ARGEEMRVEA EAREMREALD ALARGVDSVS QRAGPLTVMP 750
VPAAPGAGGR APCPPALGPE AIQARLEDVR IQARRAIESA VKEYFHRGAV 800
YSAKALQASD SHDCRFHVAS AAVVPMVQLL ESLPAFDQHT RDVAQRAALP 850
PPPPLATSPQ AILLRDLLQR GQPLDAPEDL AAWLSVLTDA ATQGLIERKP 900
LEELARSIHG INDQQARRSS GLAELQRFDA LDAALAQQLD SDAAFVPATG 950
PAPYVDGGGL SPEATRMAED ALRQARAMEA AKMTAELAPE ARSRLRERAH 1000
ALEAMLNDAR ERAKVAHDAR EKFLHKLQGV LRPLPDFVGL KACPAVLATL 1050
RASLPAGWTD LADAVRGPPP EVTAALRADL WGLLGQYREA LEHPTPDTAT 1100
ALAGLHPAFV VVLKTLFADA PETPVLVQFF SDHAPTIAKA VSNAINAGSA 1150
AVATASPAAT VDAAVRAHGA LADAVSALGA AARDPASPLS FLAVLADSAA 1200
GYVKATRLAL EARGAIDELT TLGSAAADLV VQARRACAQP EGDHAALIDA 1250
AARATTAARE SLAGHEAGFG GLLHAEGTAG DHSPSGRALQ ELGKVIGATR 1300
RRADELEAAV ADLTAKMAAQ RARGSSERWA AGVEAALDRV ENRAEFDVVE 1350
LRRLQALAGT HGYNPRDFRK RAEQALAANA EAVTLALDTA FAFNPYTPEN 1400
QRHPMLPPLA AIHRLGWSAA FHAAAETYAD MFRVDAEPLA RLLRIAEGLL 1450
EMAQAGDGFI DYHEAVGRLA DDMTSVPGLR RYVPFFQHGY ADYVELRDRL 1500
DAIRADVHRA LGGVPLDLAA AAEQISAARN DPEATAELVR TGVTLPCPSE 1550
DALVACAAAL ERVDQSPVKN TAYAEYVAFV TRQDTAETKD AVVRAKQQRA 1600
EATERVMAGL REALAARERR AQIEAEGLAN LKTMLKVVAV PATVAKTLDQ 1650
ARSVAEIADQ VEVLLDQTEK TRELDVPAVI WLEHAQRTFE THPLSAARGD 1700
GPGPLARHAG RLGALFDTRR RVDALRRSLE EAEAEWDEVW GRFGRVRGGA 1750
WKSPEGFRAM HEQLRALQDT TNTVSGLRAQ PAYERLSARY QGVLGAKGAE 1800
RAEAVEELGA RVTKHTALCA RLRDEVVRRV PWEMNFDALG GLLAEFDAAA 1850
ADLAPWAVEE FRGARELIQY RMGLYSAYAR AGGQTGAGAE SAPAPLLVDL 1900
RALDARARAS SSPEGHEVDP QLLRRRGEAY LRAGGDPGPL VLREAVSALD 1950
LPFATSFLAP DGTPLQYALC FPAVTDKLGA LLMRPEAACV RPPLPTDVLE 2000
SAPTVTAMYV LTVVNRLQLA LSDAQAANFQ LFGRFVRHRQ ATWGASMDAA 2050
AELYVALVAT TLTREFGCRW AQLGWASGAA APRPPPGPRG SQRHCVAFNE 2100
NDVLVALVAG VPEHIYNFWR LDLVRQHEYM HLTLERAFED AAESMLFVQR 2150
LTPHPDARIR VLPTFLDGGP PTRGLLFGTR LADWRRGKLS ETDPLAPWRS 2200
ALELGTQRRD VPALGKLSPA QALAAVSVLG RMCLPSAALA ALWTCMFPDD 2250
YTEYDSFDAL LAARLESGQT LGPAGGREAS LPEAPHALYR PTGQHVAVLA 2300
AATHRTPAAR VTAMDLVLAA VLLGAPVVVA LRNTTAFSRE SELELCLTLF 2350
DSRPGGPDAA LRDVVSSDIE TWAVGLLHTD LNPIENACLA AQLPRLSALI 2400
AERPLADGPP CLVLVDISMT PVAVLWEAPE PPGPPDVRFV GSEATEELPF 2450
VATAGDVLAA SAADADPFFA RAILGRPFDA SLLTGELFPG HPVYQRPLAD 2500
EAGPSAPTAA RDPRDLAGGD GGSGPEDPAA PPARQADPGV LAPTLLTDAT 2550
TGEPVPPRMW AWIHGLEELA SDDAGGPTPN PAPALLPPPA TDQSVPTSQY 2600
APRPIGPAAT ARETRPSVPP QQNTGRVPVA PRDDPRPSPP TPSPPADAAL 2650
PPPAFSGSAA AFSAAVPRVR RSRRTRAKSR APRASAPPEG WRPPALPAPV 2700
APVAASARPP DQPPTPESAP PAWVSALPLP PGPASARGAF PAPTLAPIPP 2750
PPAEGAVVPG GDRRRGRRQT TAGPSPTPPR GPAAGPPRRL TRPAVASLSA 2800
SLNSLPSPRD PADHAAAVSA AAAAVPPSPG LAPPTSAVQT SPPPLAPGPV 2850
APSEPLCGWV VPGGPVARRP PPQSPATKPA ARTRIRARSV PQPPLPQPPL 2900
PQPPLPQPPL PQPPLPQPPL PQPPLPQPPL PQPPLPQPPL PQPPLPPVTR 2950
TLTPQSRDSV PTPESPTHTN THLPVSAVTS WASSLALHVD SAPPPASLLQ 3000
TLHISSDDEH SDADSLRFSD SDDTEALDPL PPEPHLPPAD EPPGPLAADH 3050
LQSPHSQFGP LPVQANAVLS RRYVRSTGRS ALAVLIRACR RIQQQLQRTR 3100
RALFQRSNAV LTSLHHVRML LG 3122
Length:3,122
Mass (Da):330,051
Last modified:May 1, 1997 - v1
Checksum:i6EBF94B51BFE8C0B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z86099 Genomic DNA. Translation: CAB06722.1.
RefSeqiNP_044506.1. NC_001798.1.

Genome annotation databases

GeneIDi1487322.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z86099 Genomic DNA. Translation: CAB06722.1 .
RefSeqi NP_044506.1. NC_001798.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C76.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1487322.

Family and domain databases

InterProi IPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of herpes simplex virus type 2."
    Dolan A., Jamieson F.E., Cunningham C., Barnett B.C., McGeoch D.J.
    J. Virol. 72:2010-2021(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_HHV2H
AccessioniPrimary (citable) accession number: P89459
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 1997
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi