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P89459

- DEN_HHV2H

UniProt

P89459 - DEN_HHV2H

Protein

Deneddylase

Gene

UL36

Organism
Human herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401PROSITE-ProRule annotation
    Active sitei174 – 1741PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: InterPro
    2. omega peptidase activity Source: InterPro

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC76.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Short name:
    pUL36
    Alternative name(s):
    Tegument protein VP1-2
    Tegument protein VP1/2
    Ubiquitin thioesterase pUL36
    Gene namesi
    ORF Names:UL36
    OrganismiHuman herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
    Taxonomic identifieri10315 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000001874: Genome

    Subcellular locationi

    Virion tegument By similarity. Host cytoplasm By similarity. Host nucleus By similarity
    Note: Tightly associated with the capsid.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 31223122DeneddylasePRO_0000385478Add
    BLAST

    Post-translational modificationi

    Proteolytically processed, possibly into several polypeptides. Enzymatic activity is only detectable following cleavage of the UL36 protein, which occurs late during viral replication By similarity.By similarity

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with UL37 By similarity.By similarity

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 238219Peptidase C76PROSITE-ProRule annotationAdd
    BLAST
    Repeati2891 – 289551
    Repeati2896 – 290052
    Repeati2901 – 290553
    Repeati2906 – 291054
    Repeati2911 – 291555
    Repeati2916 – 292056
    Repeati2921 – 292557
    Repeati2926 – 293058
    Repeati2931 – 293559
    Repeati2936 – 2940510
    Repeati2941 – 2945511

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2891 – 29455511 X 5 AA tandem repeats of P-Q-P-P-LAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili709 – 73931Sequence AnalysisAdd
    BLAST
    Coiled coili963 – 102260Sequence AnalysisAdd
    BLAST
    Coiled coili1287 – 135468Sequence AnalysisAdd
    BLAST
    Coiled coili1586 – 161631Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi394 – 4018Nuclear localization signalBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi284 – 494211Pro-richAdd
    BLAST
    Compositional biasi850 – 8545Poly-Pro
    Compositional biasi1519 – 15224Poly-Ala
    Compositional biasi1848 – 18514Poly-Ala
    Compositional biasi2525 – 2966442Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Family and domain databases

    InterProiIPR005210. Herpes_UL36.
    IPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    PF03586. Herpes_UL36. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P89459-1 [UniParc]FASTAAdd to Basket

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    MIPAALPHPT MKRQGDRDIV VTGVRNQFAT DLEPGGSVSC MRSSLSFLSL     50
    LFDVGPRDVL SAEAIEGCLV EGGEWTRAAA GSGPPRMCSI IELPNFLEYP 100
    AARGGLRCVF SRVYGEVGFF GEPTAGLLET QCPAHTFFAG PWAMRPLSYT 150
    LLTIGPLGMG LYRDGDTAYL FDPHGLPAGT PAFIAKVRAG DVYPYLTYYA 200
    HDRPKVRWAG AMVFFVPSGP GAVAPADLTA AALHLYGASE TYLQDEPFVE 250
    RRVAITHPLR GEIGGLGALF VGVVPRGDGE GSGPVVPALP APTHVQTPGA 300
    DRPPEAPRGA SGPPDTPQAG HPNRPPDDVW AAALEGTPPA KPSAPDAAAS 350
    GPPHAAPPPQ TPAGDAAEEA EDLRVLEVGA VPVGRHRARY STGLPKRRRP 400
    TWTPPSSVED LTSGERPAPK APPAKAKKKS APKKKAPVAA EVPASSPTPI 450
    AATVPPAPDT PPQSGQGGGD DGPASPSSPS VLETLGARRP PEPPGADLAQ 500
    LFEVHPNVAA TAVRLAARDA ALAREVAACS QLTINALRSP YPAHPGLLEL 550
    CVIFFFERVL AFLIENGART HTQAGVAGPA AALLDFTLRM LPRKTAVGDF 600
    LASTRMSLAD VAAHRPLIQH VLDENSQIGR LALAKLVLVA RDVIRETDAF 650
    YGDLADLDLQ LRAAPPANLY ARLGEWLLER SRAHPNTLFA PATPTHPEPL 700
    LHRIQALAQF ARGEEMRVEA EAREMREALD ALARGVDSVS QRAGPLTVMP 750
    VPAAPGAGGR APCPPALGPE AIQARLEDVR IQARRAIESA VKEYFHRGAV 800
    YSAKALQASD SHDCRFHVAS AAVVPMVQLL ESLPAFDQHT RDVAQRAALP 850
    PPPPLATSPQ AILLRDLLQR GQPLDAPEDL AAWLSVLTDA ATQGLIERKP 900
    LEELARSIHG INDQQARRSS GLAELQRFDA LDAALAQQLD SDAAFVPATG 950
    PAPYVDGGGL SPEATRMAED ALRQARAMEA AKMTAELAPE ARSRLRERAH 1000
    ALEAMLNDAR ERAKVAHDAR EKFLHKLQGV LRPLPDFVGL KACPAVLATL 1050
    RASLPAGWTD LADAVRGPPP EVTAALRADL WGLLGQYREA LEHPTPDTAT 1100
    ALAGLHPAFV VVLKTLFADA PETPVLVQFF SDHAPTIAKA VSNAINAGSA 1150
    AVATASPAAT VDAAVRAHGA LADAVSALGA AARDPASPLS FLAVLADSAA 1200
    GYVKATRLAL EARGAIDELT TLGSAAADLV VQARRACAQP EGDHAALIDA 1250
    AARATTAARE SLAGHEAGFG GLLHAEGTAG DHSPSGRALQ ELGKVIGATR 1300
    RRADELEAAV ADLTAKMAAQ RARGSSERWA AGVEAALDRV ENRAEFDVVE 1350
    LRRLQALAGT HGYNPRDFRK RAEQALAANA EAVTLALDTA FAFNPYTPEN 1400
    QRHPMLPPLA AIHRLGWSAA FHAAAETYAD MFRVDAEPLA RLLRIAEGLL 1450
    EMAQAGDGFI DYHEAVGRLA DDMTSVPGLR RYVPFFQHGY ADYVELRDRL 1500
    DAIRADVHRA LGGVPLDLAA AAEQISAARN DPEATAELVR TGVTLPCPSE 1550
    DALVACAAAL ERVDQSPVKN TAYAEYVAFV TRQDTAETKD AVVRAKQQRA 1600
    EATERVMAGL REALAARERR AQIEAEGLAN LKTMLKVVAV PATVAKTLDQ 1650
    ARSVAEIADQ VEVLLDQTEK TRELDVPAVI WLEHAQRTFE THPLSAARGD 1700
    GPGPLARHAG RLGALFDTRR RVDALRRSLE EAEAEWDEVW GRFGRVRGGA 1750
    WKSPEGFRAM HEQLRALQDT TNTVSGLRAQ PAYERLSARY QGVLGAKGAE 1800
    RAEAVEELGA RVTKHTALCA RLRDEVVRRV PWEMNFDALG GLLAEFDAAA 1850
    ADLAPWAVEE FRGARELIQY RMGLYSAYAR AGGQTGAGAE SAPAPLLVDL 1900
    RALDARARAS SSPEGHEVDP QLLRRRGEAY LRAGGDPGPL VLREAVSALD 1950
    LPFATSFLAP DGTPLQYALC FPAVTDKLGA LLMRPEAACV RPPLPTDVLE 2000
    SAPTVTAMYV LTVVNRLQLA LSDAQAANFQ LFGRFVRHRQ ATWGASMDAA 2050
    AELYVALVAT TLTREFGCRW AQLGWASGAA APRPPPGPRG SQRHCVAFNE 2100
    NDVLVALVAG VPEHIYNFWR LDLVRQHEYM HLTLERAFED AAESMLFVQR 2150
    LTPHPDARIR VLPTFLDGGP PTRGLLFGTR LADWRRGKLS ETDPLAPWRS 2200
    ALELGTQRRD VPALGKLSPA QALAAVSVLG RMCLPSAALA ALWTCMFPDD 2250
    YTEYDSFDAL LAARLESGQT LGPAGGREAS LPEAPHALYR PTGQHVAVLA 2300
    AATHRTPAAR VTAMDLVLAA VLLGAPVVVA LRNTTAFSRE SELELCLTLF 2350
    DSRPGGPDAA LRDVVSSDIE TWAVGLLHTD LNPIENACLA AQLPRLSALI 2400
    AERPLADGPP CLVLVDISMT PVAVLWEAPE PPGPPDVRFV GSEATEELPF 2450
    VATAGDVLAA SAADADPFFA RAILGRPFDA SLLTGELFPG HPVYQRPLAD 2500
    EAGPSAPTAA RDPRDLAGGD GGSGPEDPAA PPARQADPGV LAPTLLTDAT 2550
    TGEPVPPRMW AWIHGLEELA SDDAGGPTPN PAPALLPPPA TDQSVPTSQY 2600
    APRPIGPAAT ARETRPSVPP QQNTGRVPVA PRDDPRPSPP TPSPPADAAL 2650
    PPPAFSGSAA AFSAAVPRVR RSRRTRAKSR APRASAPPEG WRPPALPAPV 2700
    APVAASARPP DQPPTPESAP PAWVSALPLP PGPASARGAF PAPTLAPIPP 2750
    PPAEGAVVPG GDRRRGRRQT TAGPSPTPPR GPAAGPPRRL TRPAVASLSA 2800
    SLNSLPSPRD PADHAAAVSA AAAAVPPSPG LAPPTSAVQT SPPPLAPGPV 2850
    APSEPLCGWV VPGGPVARRP PPQSPATKPA ARTRIRARSV PQPPLPQPPL 2900
    PQPPLPQPPL PQPPLPQPPL PQPPLPQPPL PQPPLPQPPL PQPPLPPVTR 2950
    TLTPQSRDSV PTPESPTHTN THLPVSAVTS WASSLALHVD SAPPPASLLQ 3000
    TLHISSDDEH SDADSLRFSD SDDTEALDPL PPEPHLPPAD EPPGPLAADH 3050
    LQSPHSQFGP LPVQANAVLS RRYVRSTGRS ALAVLIRACR RIQQQLQRTR 3100
    RALFQRSNAV LTSLHHVRML LG 3122
    Length:3,122
    Mass (Da):330,051
    Last modified:May 1, 1997 - v1
    Checksum:i6EBF94B51BFE8C0B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z86099 Genomic DNA. Translation: CAB06722.1.
    RefSeqiNP_044506.1. NC_001798.1.

    Genome annotation databases

    GeneIDi1487322.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z86099 Genomic DNA. Translation: CAB06722.1 .
    RefSeqi NP_044506.1. NC_001798.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C76.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1487322.

    Family and domain databases

    InterProi IPR005210. Herpes_UL36.
    IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    PF03586. Herpes_UL36. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of herpes simplex virus type 2."
      Dolan A., Jamieson F.E., Cunningham C., Barnett B.C., McGeoch D.J.
      J. Virol. 72:2010-2021(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiDEN_HHV2H
    AccessioniPrimary (citable) accession number: P89459
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3