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Protein

Capsid scaffolding protein

Gene

UL26

Organism
Human herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.UniRule annotation
Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.UniRule annotation
Assembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.UniRule annotation

Catalytic activityi

Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei61 – 611Charge relay systemUniRule annotation
Active sitei129 – 1291Charge relay systemUniRule annotation
Active sitei148 – 1481Charge relay systemUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Viral capsid assembly, Virus exit from host cell

Protein family/group databases

MEROPSiS21.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid scaffolding proteinUniRule annotation
Alternative name(s):
Capsid protein P40
Protease precursorUniRule annotation
Short name:
pPRUniRule annotation
Virion structural protein UL26
Cleaved into the following 2 chains:
AssemblinUniRule annotation (EC:3.4.21.97UniRule annotation)
Alternative name(s):
Capsid protein VP24
ProteaseUniRule annotation
Assembly proteinUniRule annotation
Alternative name(s):
Capsid assembly proteinUniRule annotation
Capsid protein VP22A
Gene namesi
Name:UL26
OrganismiHuman herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Taxonomic identifieri10315 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000001874 Componenti: Genome

Subcellular locationi

Capsid scaffolding protein :
  • Host cytoplasm UniRule annotation
Assemblin :
  • Host nucleus UniRule annotation
Assembly protein :
  • Host nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3416.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637Capsid scaffolding proteinPRO_0000406172Add
BLAST
Chaini1 – 247247AssemblinUniRule annotationPRO_0000406173Add
BLAST
Chaini248 – 637390Assembly proteinUniRule annotationPRO_0000406174Add
BLAST

Post-translational modificationi

Capsid scaffolding protein is cleaved by assemblin after formation of the spherical procapsid. As a result, the capsid obtains its mature, icosahedral shape. Cleavages occur at two or more sites: release and tail site.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei247 – 2482Cleavage; by assemblin; Release siteUniRule annotation
Sitei612 – 6132Cleavage; by assemblin; Tail siteUniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Capsid scaffolding protein homomultimerizes and interacts with major capsid protein. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein.UniRule annotation

Chemistry

BindingDBiP89449.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni326 – 34520Interaction with pAPUniRule annotationAdd
BLAST
Regioni617 – 63721Interaction with major capsid proteinUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi428 – 4314Nuclear localization signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi296 – 3027Poly-Ser
Compositional biasi318 – 3236Poly-Pro
Compositional biasi376 – 3794Poly-Pro
Compositional biasi516 – 59277Pro-richAdd
BLAST
Compositional biasi581 – 59212Poly-ProAdd
BLAST

Domaini

Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD).UniRule annotation

Sequence similaritiesi

Belongs to the herpesviridae capsid scaffolding protein family.UniRule annotation

Phylogenomic databases

KOiK05747.

Family and domain databases

HAMAPiMF_04008. HSV_SCAF.
InterProiIPR001847. Peptidase_S21.
[Graphical view]
PfamiPF00716. Peptidase_S21. 1 hit.
[Graphical view]
PRINTSiPR00236. HSVCAPSIDP40.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform Capsid scaffolding protein (identifier: P89449-1) [UniParc]FASTAAdd to basket

Also known as: pPR, UL26

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASAEMRERL EAPLPDRAVP IYVAGFLALY DSGDPGELAL DPDTVRAALP
60 70 80 90 100
PENPLPINVD HRARCEVGRV LAVVNDPRGP FFVGLIACVQ LERVLETAAS
110 120 130 140 150
AAIFERRGPA LSREERLLYL ITNYLPSVSL STKRRGDEVP PDRTLFAHVA
160 170 180 190 200
LCAIGRRLGT IVTYDTSLDA AIAPFRHLDP ATREGVRREA AEAELALAGR
210 220 230 240 250
TWAPGVEALT HTLLSTAVNN MMLRDRWSLV AERRRQAGIA GHTYLQASEK
260 270 280 290 300
FKIWGAESAP APERGYKTGA PGAMDTSPAA SVPAPQVAVR ARQVASSSSS
310 320 330 340 350
SSFPAPADMN PVSASGAPAP PPPGDGSYLW IPASHYNQLV TGQSAPRHPP
360 370 380 390 400
LTACGLPAAG TVAYGHPGAG PSPHYPPPPA HPYPGMLFAG PSPLEAQIAA
410 420 430 440 450
LVGAIAADRQ AGGLPAAAGD HGIRGSAKRR RHEVEQPEYD CGRDEPDRDF
460 470 480 490 500
PYYPGEARPE PRPVDSRRAA RQASGPHETI TALVGAVTSL QQELAHMRAR
510 520 530 540 550
THAPYGPYPP VGPYHHPHAD TETPAQPPRY PAKAVYLPPP HIAPPGPPLS
560 570 580 590 600
GAVPPPSYPP VAVTPGPAPP LHQPSPAHAH PPPPPPGPTP PPAASLPQPE
610 620 630
APGAEAGALV NASSAAHVNV DTARAADLFV SQMMGSR
Length:637
Mass (Da):66,941
Last modified:May 1, 1997 - v1
Checksum:i96B9D1F68C8DAB9C
GO
Isoform pAP (identifier: P89449-2) [UniParc]FASTAAdd to basket

Also known as: Assembly protein, UL26.5 protein

The sequence of this isoform differs from the canonical sequence as follows:
     1-308: Missing.

Show »
Length:329
Mass (Da):33,836
Checksum:i59B0B428E63451E6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 308308Missing in isoform pAP. CuratedVSP_040777Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z86099 Genomic DNA. Translation: CAB06750.1.
Z86099 Genomic DNA. Translation: CAB06751.1. Sequence problems.
RefSeqiYP_009137177.1. NC_001798.2.
YP_009137178.1. NC_001798.2.

Genome annotation databases

GeneIDi1487310.
1487311.
KEGGivg:1487310.
vg:1487311.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z86099 Genomic DNA. Translation: CAB06750.1.
Z86099 Genomic DNA. Translation: CAB06751.1. Sequence problems.
RefSeqiYP_009137177.1. NC_001798.2.
YP_009137178.1. NC_001798.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP89449.
ChEMBLiCHEMBL3416.

Protein family/group databases

MEROPSiS21.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1487310.
1487311.
KEGGivg:1487310.
vg:1487311.

Phylogenomic databases

KOiK05747.

Family and domain databases

HAMAPiMF_04008. HSV_SCAF.
InterProiIPR001847. Peptidase_S21.
[Graphical view]
PfamiPF00716. Peptidase_S21. 1 hit.
[Graphical view]
PRINTSiPR00236. HSVCAPSIDP40.
ProtoNetiSearch...

Publicationsi

  1. "Comparative sequence analysis of the long repeat regions and adjoining parts of the long unique regions in the genomes of herpes simplex viruses types 1 and 2."
    McGeoch D.J., Cunningham C., McIntyre G., Dolan A.
    J. Gen. Virol. 72:3057-3075(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSCAF_HHV2H
AccessioniPrimary (citable) accession number: P89449
Secondary accession number(s): P90341
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: May 1, 1997
Last modified: April 13, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.