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P89446 (KITH_HHV2H) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase

EC=2.7.1.21
Gene names
Name:TK
ORF Names:UL23
OrganismHuman herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2) [Reference proteome]
Taxonomic identifier10315 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome By similarity.

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subunit structure

Homodimer By similarity.

Miscellaneous

Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HSV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions By similarity.

Sequence similarities

Belongs to the herpesviridae thymidine kinase family.

Ontologies

Keywords
   Biological processDNA synthesis
   Developmental stageEarly protein
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

TMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 376376Thymidine kinase
PRO_0000385457

Regions

Nucleotide binding56 – 638ATP By similarity

Sites

Active site841Proton acceptor Potential
Binding site1021Substrate By similarity
Binding site1261Substrate By similarity
Binding site1731Substrate By similarity
Binding site2171ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P89446 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: E70AE9F26E226A44

FASTA37640,395
        10         20         30         40         50         60 
MASHAGQQHA PAFGQAARAS GPTDGRAASR PSHRQGASGA RGDPELPTLL RVYIDGPHGV 

        70         80         90        100        110        120 
GKTTTSAQLM EALGPRDNIV YVPEPMTYWQ VLGASETLTN IYNTQHRLDR GEISAGEAAV 

       130        140        150        160        170        180 
VMTSAQITMS TPYAATDAVL APHIGGEAVG PQAPPPALTL VFDRHPIASL LCYPAARYLM 

       190        200        210        220        230        240 
GSMTPQAVLA FVALMPPTAP GTNLVLGVLP EAEHADRLAR RQRPGERLDL AMLSAIRRVY 

       250        260        270        280        290        300 
DLLANTVRYL QRGGRWREDW GRLTGVAAAT PRPDPEDGAG SLPRIEDTLF ALFRVPELLA 

       310        320        330        340        350        360 
PNGDLYHIFA WVLDVLADRL LPMHLFVLDY DQSPVGCRDA LLRLTAGMIP TRVTTAGSIA 

       370 
EIRDLARTFA REVGGV 

« Hide

References

[1]"The genome sequence of herpes simplex virus type 2."
Dolan A., Jamieson F.E., Cunningham C., Barnett B.C., McGeoch D.J.
J. Virol. 72:2010-2021(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z86099 Genomic DNA. Translation: CAB06747.1.
RefSeqNP_044492.1. NC_001798.1.

3D structure databases

ProteinModelPortalP89446.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP89446.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1487307.

Phylogenomic databases

ProtClustDBPHA3133.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKITH_HHV2H
AccessionPrimary (citable) accession number: P89446
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families