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P89446

- KITH_HHV2H

UniProt

P89446 - KITH_HHV2H

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Protein

Thymidine kinase

Gene

TK

Organism
Human herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841Proton acceptorSequence Analysis
Binding sitei102 – 1021SubstrateBy similarity
Binding sitei126 – 1261SubstrateBy similarity
Binding sitei173 – 1731SubstrateBy similarity
Binding sitei217 – 2171ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi56 – 638ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. TMP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
ORF Names:UL23
OrganismiHuman herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2)
Taxonomic identifieri10315 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001874: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Thymidine kinasePRO_0000385457Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP89446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P89446-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MASHAGQQHA PAFGQAARAS GPTDGRAASR PSHRQGASGA RGDPELPTLL
60 70 80 90 100
RVYIDGPHGV GKTTTSAQLM EALGPRDNIV YVPEPMTYWQ VLGASETLTN
110 120 130 140 150
IYNTQHRLDR GEISAGEAAV VMTSAQITMS TPYAATDAVL APHIGGEAVG
160 170 180 190 200
PQAPPPALTL VFDRHPIASL LCYPAARYLM GSMTPQAVLA FVALMPPTAP
210 220 230 240 250
GTNLVLGVLP EAEHADRLAR RQRPGERLDL AMLSAIRRVY DLLANTVRYL
260 270 280 290 300
QRGGRWREDW GRLTGVAAAT PRPDPEDGAG SLPRIEDTLF ALFRVPELLA
310 320 330 340 350
PNGDLYHIFA WVLDVLADRL LPMHLFVLDY DQSPVGCRDA LLRLTAGMIP
360 370
TRVTTAGSIA EIRDLARTFA REVGGV
Length:376
Mass (Da):40,395
Last modified:May 1, 1997 - v1
Checksum:iE70AE9F26E226A44
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z86099 Genomic DNA. Translation: CAB06747.1.
RefSeqiNP_044492.1. NC_001798.1.

Genome annotation databases

GeneIDi1487307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z86099 Genomic DNA. Translation: CAB06747.1 .
RefSeqi NP_044492.1. NC_001798.1.

3D structure databases

ProteinModelPortali P89446.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P89446.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1487307.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00693. Herpes_TK. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of herpes simplex virus type 2."
    Dolan A., Jamieson F.E., Cunningham C., Barnett B.C., McGeoch D.J.
    J. Virol. 72:2010-2021(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiKITH_HHV2H
AccessioniPrimary (citable) accession number: P89446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 1997
Last modified: October 1, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HSV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3