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Protein

RNA polymerase-associated protein CTR9

Gene

CTR9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA-binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of RAD6 ubiquitin conjugate and the BRE1 ubiquitin ligase which ubiquitinate 'Lys-126' histone H2B. Activates the SET1 histone methyltransferase complex for methylation of 'Lys-4' of histone H3 and for methylation of 'Lys-73' of histone H3 by DOT1 and 'Lys-36' of histone H3 by SET2. In complex with PAF1, required for normal CLN1 and CLN2 G1 cyclin expression in late G1. Also has a role in chromosome segregation where it appears to be involved in microtubule placement.4 Publications

GO - Molecular functioni

  • RNA polymerase II core binding Source: SGD
  • RNA polymerase II C-terminal domain phosphoserine binding Source: SGD
  • transcription factor activity, RNA polymerase II transcription factor binding Source: SGD
  • triplex DNA binding Source: SGD

GO - Biological processi

  • DNA-templated transcription, termination Source: SGD
  • histone modification Source: InterPro
  • mRNA 3'-end processing Source: SGD
  • positive regulation of histone H3-K36 trimethylation Source: SGD
  • positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues Source: SGD
  • positive regulation of transcription elongation from RNA polymerase I promoter Source: SGD
  • regulation of chromatin silencing at telomere Source: SGD
  • regulation of histone H2B conserved C-terminal lysine ubiquitination Source: SGD
  • regulation of histone H3-K4 methylation Source: SGD
  • regulation of transcription-coupled nucleotide-excision repair Source: SGD
  • regulation of transcription initiation from RNA polymerase II promoter Source: SGD
  • regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  • snoRNA 3'-end processing Source: SGD
  • snoRNA transcription from an RNA polymerase II promoter Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
  • transcription elongation from RNA polymerase I promoter Source: SGD
  • transcription from RNA polymerase I promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33535-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase-associated protein CTR9
Alternative name(s):
Centromere-binding factor 1-dependent protein 1
Cln three-requiring protein 9
Gene namesi
Name:CTR9
Synonyms:CDP1
Ordered Locus Names:YOL145C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL145C.
SGDiS000005505. CTR9.

Subcellular locationi

GO - Cellular componenti

  • Cdc73/Paf1 complex Source: SGD
  • nucleus Source: SGD
  • transcriptionally active chromatin Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001062801 – 1077RNA polymerase-associated protein CTR9Add BLAST1077

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1015PhosphoserineCombined sources1
Modified residuei1017PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP89105.
PRIDEiP89105.

PTM databases

iPTMnetiP89105.

Interactioni

Subunit structurei

Component of the PAF1 complex which consists of at least CDC73, CTR9, LEO1, PAF1 and RTF1. Interacts with SPT6. Interacts with FACT subunits POB3 and SPT16.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LEO1P384396EBI-5283,EBI-10108
PAF1P383516EBI-5283,EBI-12855
RPO21P040504EBI-5283,EBI-15760
RTF1P5306410EBI-5283,EBI-16303
SLT2Q007722EBI-5283,EBI-17372
SPT5P276922EBI-5283,EBI-17937

GO - Molecular functioni

  • RNA polymerase II core binding Source: SGD
  • RNA polymerase II C-terminal domain phosphoserine binding Source: SGD

Protein-protein interaction databases

BioGridi34272. 175 interactors.
DIPiDIP-2814N.
IntActiP89105. 34 interactors.
MINTiMINT-483774.

Structurei

3D structure databases

ProteinModelPortaliP89105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati56 – 89TPR 1Add BLAST34
Repeati138 – 174TPR 2Add BLAST37
Repeati183 – 216TPR 3Add BLAST34
Repeati218 – 251TPR 4Add BLAST34
Repeati298 – 332TPR 5Add BLAST35
Repeati338 – 371TPR 6Add BLAST34
Repeati373 – 405TPR 7Add BLAST33
Repeati421 – 455TPR 8Add BLAST35
Repeati462 – 495TPR 9Add BLAST34
Repeati501 – 534TPR 10Add BLAST34
Repeati540 – 572TPR 11Add BLAST33
Repeati664 – 697TPR 12Add BLAST34
Repeati699 – 731TPR 13Add BLAST33
Repeati732 – 764TPR 14Add BLAST33
Repeati768 – 801TPR 15Add BLAST34
Repeati830 – 863TPR 16Add BLAST34

Sequence similaritiesi

Contains 16 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

GeneTreeiENSGT00390000005097.
HOGENOMiHOG000248464.
InParanoidiP89105.
KOiK15176.
OMAiEYNIART.
OrthoDBiEOG092C0EHT.

Family and domain databases

Gene3Di1.25.40.10. 5 hits.
InterProiIPR031101. Ctr9.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR14027:SF2. PTHR14027:SF2. 1 hit.
PfamiPF13174. TPR_6. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 8 hits.
PS50293. TPR_REGION. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P89105-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNAMKVEGY PSMEWPTSLD IPLKASEELV GIDLETDLPD DPTDLKTLLV
60 70 80 90 100
EENSEKEHWL TIALAYCNHG KTNEGIKLIE MALDVFQNSE RASLHTFLTW
110 120 130 140 150
AHLNLAKGQS LSVETKEHEL TQAELNLKDA IGFDPTWIGN MLATVELYYQ
160 170 180 190 200
RGHYDKALET SDLFVKSIHA EDHRSGRQSK PNCLFLLLRA KLLYQKKNYM
210 220 230 240 250
ASLKIFQELL VINPVLQPDP RIGIGLCFWQ LKDSKMAIKS WQRALQLNPK
260 270 280 290 300
NTSASILVLL GEFRESFTNS TNDKTFKEAF TKALSDLNNI FSENQHNPVL
310 320 330 340 350
LTLLQTYYYF KGDYQTVLDI YHHRILKMSP MIAKIVLSES SFWCGRAHYA
360 370 380 390 400
LGDYRKSFIM FQESLKKNED NLLAKLGLGQ TQIKNNLLEE SIITFENLYK
410 420 430 440 450
TNESLQELNY ILGMLYAGKA FDAKTAKNTS AKEQSNLNEK ALKYLERYLK
460 470 480 490 500
LTLATKNQLV ISRAYLVISQ LYELQNQYKT SLDYLSKALE EMEFIKKEIP
510 520 530 540 550
LEVLNNLACY HFINGDFIKA DDLFKQAKAK VSDKDESVNI TLEYNIARTN
560 570 580 590 600
EKNDCEKSES IYSQVTSLHP AYIAARIRNL YLKFAQSKIE DSDMSTEMNK
610 620 630 640 650
LLDLNKSDLE IRSFYGWYLK NSKERKNNEK STTHNKETLV KYNSHDAYAL
660 670 680 690 700
ISLANLYVTI ARDGKKSRNP KEQEKSKHSY LKAIQLYQKV LQVDPFNIFA
710 720 730 740 750
AQGLAIIFAE SKRLGPALEI LRKVRDSLDN EDVQLNLAHC YLEMREYGKA
760 770 780 790 800
IENYELVLKK FDNEKTRPHI LNLLGRAWYA RAIKERSVNF YQKALENAKT
810 820 830 840 850
ALDLFVKESS KSKFIHSVKF NIALLHFQIA ETLRRSNPKF RTVQQIKDSL
860 870 880 890 900
EGLKEGLELF RELNDLKEFN MIPKEELEQR IQLGETTMKS ALERSLNEQE
910 920 930 940 950
EFEKEQSAKI DEARKILEEN ELKEQGWMKQ EEEARRLKLE KQAEEYRKLQ
960 970 980 990 1000
DEAQKLIQER EAMAISEHNV KDDSDLSDKD NEYDEEKPRQ KRKRSTKTKN
1010 1020 1030 1040 1050
SGESKRRKAA KKTLSDSDED DDDVVKKPSH NKGKKSQLSN EFIEDSDEEE
1060 1070
AQMSGSEQNK NDDNDENNDN DDNDGLF
Length:1,077
Mass (Da):124,709
Last modified:September 21, 2011 - v3
Checksum:iF794ECBAD26D4820
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197K → E in CAA88282 (PubMed:8553699).Curated1
Sequence conflicti197K → E in CAA99166 (PubMed:9169874).Curated1
Sequence conflicti461I → V in AAB81882 (PubMed:8978028).Curated1
Sequence conflicti674E → G in CAA88282 (PubMed:8553699).Curated1
Sequence conflicti674E → G in CAA99166 (PubMed:9169874).Curated1
Sequence conflicti786R → T in CAA88282 (PubMed:8553699).Curated1
Sequence conflicti786R → T in CAA99166 (PubMed:9169874).Curated1
Sequence conflicti903E → K in CAA88282 (PubMed:8553699).Curated1
Sequence conflicti903E → K in CAA99166 (PubMed:9169874).Curated1
Sequence conflicti907S → R in CAA88282 (PubMed:8553699).Curated1
Sequence conflicti907S → R in CAA99166 (PubMed:9169874).Curated1
Sequence conflicti926 – 927GW → ER in AAB38704 (Ref. 1) Curated2
Sequence conflicti926 – 927GW → ER in AAB81882 (PubMed:8978028).Curated2
Sequence conflicti956 – 959LIQE → IFQV in CAA88282 (PubMed:8553699).Curated4
Sequence conflicti956 – 959LIQE → IFQV in CAA99166 (PubMed:9169874).Curated4
Sequence conflicti987K → Q in CAA88282 (PubMed:8553699).Curated1
Sequence conflicti987K → Q in CAA99166 (PubMed:9169874).Curated1
Sequence conflicti1045 – 1077DSDEE…NDGLF → R in CAA88282 (PubMed:8553699).CuratedAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69264 Genomic DNA. Translation: AAB38704.1.
U31217 Genomic DNA. Translation: AAB81882.1.
Z48239 Genomic DNA. Translation: CAA88282.1.
Z74887 Genomic DNA. Translation: CAA99166.1.
BK006948 Genomic DNA. Translation: DAA10640.2.
PIRiS66842.
RefSeqiNP_014496.2. NM_001183399.2.

Genome annotation databases

EnsemblFungiiYOL145C; YOL145C; YOL145C.
GeneIDi854020.
KEGGisce:YOL145C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69264 Genomic DNA. Translation: AAB38704.1.
U31217 Genomic DNA. Translation: AAB81882.1.
Z48239 Genomic DNA. Translation: CAA88282.1.
Z74887 Genomic DNA. Translation: CAA99166.1.
BK006948 Genomic DNA. Translation: DAA10640.2.
PIRiS66842.
RefSeqiNP_014496.2. NM_001183399.2.

3D structure databases

ProteinModelPortaliP89105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34272. 175 interactors.
DIPiDIP-2814N.
IntActiP89105. 34 interactors.
MINTiMINT-483774.

PTM databases

iPTMnetiP89105.

Proteomic databases

MaxQBiP89105.
PRIDEiP89105.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL145C; YOL145C; YOL145C.
GeneIDi854020.
KEGGisce:YOL145C.

Organism-specific databases

EuPathDBiFungiDB:YOL145C.
SGDiS000005505. CTR9.

Phylogenomic databases

GeneTreeiENSGT00390000005097.
HOGENOMiHOG000248464.
InParanoidiP89105.
KOiK15176.
OMAiEYNIART.
OrthoDBiEOG092C0EHT.

Enzyme and pathway databases

BioCyciYEAST:G3O-33535-MONOMER.

Miscellaneous databases

PROiP89105.

Family and domain databases

Gene3Di1.25.40.10. 5 hits.
InterProiIPR031101. Ctr9.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR14027:SF2. PTHR14027:SF2. 1 hit.
PfamiPF13174. TPR_6. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 8 hits.
PS50293. TPR_REGION. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTR9_YEAST
AccessioniPrimary (citable) accession number: P89105
Secondary accession number(s): D6W1S4
, O14409, Q07332, Q08292
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 21, 2011
Last modified: November 30, 2016
This is version 166 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.