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Protein

E4-ORF1

Gene

E4

Organism
Human adenovirus D serotype 9 (HAdV-9) (Human adenovirus 9)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in virus oncogenecity in animals. Binds and sequesters human MUPP1/MPDZ protein in the cytoplasm, preventing it from playing a role in cellular proliferation regulation. Induces cell transformation, probably by inactivating MPDZ protein.1 Publication

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Host-virus interaction, Nucleotide metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
E4-ORF1 (EC:3.6.1.23)
Alternative name(s):
Early E4 14.0 kDa protein
ORF1
Probable dUTPase E4 ORF1
Gene namesi
Name:E4
OrganismiHuman adenovirus D serotype 9 (HAdV-9) (Human adenovirus 9)
Taxonomic identifieri10527 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirusHuman mastadenovirus D
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

  • Host cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 418FHIPPHGV → IHIPPQGA: Complete loss of transformation. 1 Publication
Mutagenesisi89 – 891L → Q: Complete loss of transformation. 1 Publication
Mutagenesisi91 – 911F → S: Complete loss of transformation. 1 Publication
Mutagenesisi122 – 1221A → D: Partial loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus. 2 Publications
Mutagenesisi123 – 1253TLV → P: Complete loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus. 2 Publications
Mutagenesisi124 – 1241L → P: Partial loss of transformation. Partial loss of binding to MPDZ. Localizes aberrantly in the nucleus. 1 Publication

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 125125E4-ORF1PRO_0000221784Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Binds to human MPDZ.

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg1Q626964EBI-7401124,EBI-389325From a different organism.

Protein-protein interaction databases

DIPiDIP-44835N.
IntActiP89079. 1 interaction.
MINTiMINT-431542.

Structurei

3D structure databases

ProteinModelPortaliP89079.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi122 – 1254PDZ-bindingSequence analysis

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.

Sequencei

Sequence statusi: Complete.

P89079-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESLYAFID SPGGIAPVQE GTSNRYTFFC PESFHIPPHG VVLLHLKVSV
60 70 80 90 100
LVPTGYQGRF MALNDYHARD ILTQSDVIFA GRRQELTVLL FNHTDRFLYV
110 120
RKGHPVGTLL LERVIFPSVK IATLV
Length:125
Mass (Da):14,041
Last modified:May 1, 1997 - v1
Checksum:i75792A75E484BC09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82508 Genomic DNA. Translation: AAB37504.1.
AJ854486 Genomic DNA. Translation: CAI05991.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82508 Genomic DNA. Translation: AAB37504.1.
AJ854486 Genomic DNA. Translation: CAI05991.1.

3D structure databases

ProteinModelPortaliP89079.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-44835N.
IntActiP89079. 1 interaction.
MINTiMINT-431542.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiE4OR1_ADE09
AccessioniPrimary (citable) accession number: P89079
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: May 1, 1997
Last modified: May 11, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.