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P89079 (E4OR1_ADE09) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E4-ORF1

EC=3.6.1.23
Alternative name(s):
Early E4 14.0 kDa protein
ORF1
Probable dUTPase E4 ORF1
Gene names
Name:E4
OrganismHuman adenovirus D serotype 9 (HAdV-9) (Human adenovirus 9)
Taxonomic identifier10527 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirusHuman adenovirus D
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length125 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a key role in virus oncogenecity in animals. Binds and sequesters human MUPP1/MPDZ protein in the cytoplasm, preventing it from playing a role in cellular proliferation regulation. Induces cell transformation, probably by inactivating MPDZ protein. Ref.3

Catalytic activity

dUTP + H2O = dUMP + diphosphate.

Subunit structure

Binds to human MPDZ. Ref.5

Subcellular location

Host cytoplasm Ref.4.

Sequence similarities

Belongs to the dUTPase family.

Ontologies

Keywords
   Biological processHost-virus interaction
Nucleotide metabolism
   Cellular componentHost cytoplasm
   Developmental stageEarly protein
   DiseaseOncogene
   Molecular functionHydrolase
Gene Ontology (GO)
   Biological_processdUTP metabolic process

Inferred from electronic annotation. Source: InterPro

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dlg1Q626964EBI-7401124,EBI-389325From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 125125E4-ORF1
PRO_0000221784

Regions

Motif122 – 1254PDZ-binding Potential

Experimental info

Mutagenesis34 – 418FHIPPHGV → IHIPPQGA: Complete loss of transformation. Ref.4
Mutagenesis891L → Q: Complete loss of transformation. Ref.4
Mutagenesis911F → S: Complete loss of transformation. Ref.4
Mutagenesis1221A → D: Partial loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus. Ref.4 Ref.5
Mutagenesis123 – 1253TLV → P: Complete loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus. Ref.4 Ref.5
Mutagenesis1241L → P: Partial loss of transformation. Partial loss of binding to MPDZ. Localizes aberrantly in the nucleus. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P89079 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 75792A75E484BC09

FASTA12514,041
        10         20         30         40         50         60 
MAESLYAFID SPGGIAPVQE GTSNRYTFFC PESFHIPPHG VVLLHLKVSV LVPTGYQGRF 

        70         80         90        100        110        120 
MALNDYHARD ILTQSDVIFA GRRQELTVLL FNHTDRFLYV RKGHPVGTLL LERVIFPSVK 


IATLV 

« Hide

References

« Hide 'large scale' references
[1]"Mammary tumors induced by human adenovirus type 9: a role for the viral early region 4 gene."
Javier R.T., Shenk T.
Breast Cancer Res. Treat. 39:57-67(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Adenovirus type 9, complete sequence."
Buettner W.H., Veres-Molnar S.K.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate ATCC VR-1086 / Hicks / V-209-003-014.
[3]"Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats."
Javier R.T.
J. Virol. 68:3917-3924(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS ONCOGENE.
[4]"Mutant adenovirus type 9 E4 ORF1 genes define three protein regions required for transformation of CREF cells."
Weiss R.S., Gold M.O., Vogel H., Javier R.T.
J. Virol. 71:4385-4394(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF 34-PHE--VAL-41; LEU-89; PHE-91; ALA-122 AND 123-THR--VAL-125.
[5]"Multi-PDZ domain protein MUPP1 is a cellular target for both adenovirus E4-ORF1 and high-risk papillomavirus type 18 E6 oncoproteins."
Lee S.S., Glaunsinger B., Mantovani F., Banks L., Javier R.T.
J. Virol. 74:9680-9693(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN MPDZ, MUTAGENESIS OF ALA-122; 123-THR--VAL-125 AND LEU-124.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S82508 Genomic DNA. Translation: AAB37504.1.
AJ854486 Genomic DNA. Translation: CAI05991.1.

3D structure databases

ProteinModelPortalP89079.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP89079. 1 interaction.
MINTMINT-431542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008180. dUTP_pyroPase.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE4OR1_ADE09
AccessionPrimary (citable) accession number: P89079
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: May 1, 1997
Last modified: February 19, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families