P89079 (E4OR1_ADE09) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E4-ORF1
EC=3.6.1.23

Alternative name(s):
Early E4 14.0 kDa protein
ORF1
Probable dUTPase E4 ORF1

Gene names

Name:

E4

Organism

Human adenovirus D serotype 9 (HAdV-9) (Human adenovirus 9)

Taxonomic identifier

10527 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Human adenovirus D

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a key role in virus oncogenecity in animals. Binds and sequesters human MUPP1/MPDZ protein in the cytoplasm, preventing it from playing a role in cellular proliferation regulation. Induces cell transformation, probably by inactivating MPDZ protein. Ref.3
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Subunit structure	Binds to human MPDZ. Ref.5
Subcellular location	Host cytoplasm Ref.4.
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Host-virus interaction Nucleotide metabolism
Cellular component	Host cytoplasm
Developmental stage	Early protein
Disease	Oncogene
Molecular function	Hydrolase
Gene Ontology (GO)
Biological_process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 125	125	E4-ORF1		PRO_0000221784
Regions
	Motif	122 – 125	4	PDZ-binding Potential
Experimental info
	Mutagenesis	34 – 41	8	FHIPPHGV → IHIPPQGA: Complete loss of transformation. Ref.4
	Mutagenesis	89	1	L → Q: Complete loss of transformation. Ref.4
	Mutagenesis	91	1	F → S: Complete loss of transformation. Ref.4
	Mutagenesis	122	1	A → D: Partial loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus. Ref.4 Ref.5
	Mutagenesis	123 – 125	3	TLV → P: Complete loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus. Ref.4 Ref.5
	Mutagenesis	124	1	L → P: Partial loss of transformation. Partial loss of binding to MPDZ. Localizes aberrantly in the nucleus. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P89079 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 75792A75E484BC09	FASTA	125	14,041
	10 20 30 40 50 60 MAESLYAFID SPGGIAPVQE GTSNRYTFFC PESFHIPPHG VVLLHLKVSV LVPTGYQGRF 70 80 90 100 110 120 MALNDYHARD ILTQSDVIFA GRRQELTVLL FNHTDRFLYV RKGHPVGTLL LERVIFPSVK IATLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammary tumors induced by human adenovirus type 9: a role for the viral early region 4 gene." Javier R.T., Shenk T. Breast Cancer Res. Treat. 39:57-67(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Adenovirus type 9, complete sequence." Buettner W.H., Veres-Molnar S.K. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate ATCC VR-1086 / Hicks / V-209-003-014.
[3]	"Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats." Javier R.T. J. Virol. 68:3917-3924(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS ONCOGENE.
[4]	"Mutant adenovirus type 9 E4 ORF1 genes define three protein regions required for transformation of CREF cells." Weiss R.S., Gold M.O., Vogel H., Javier R.T. J. Virol. 71:4385-4394(1997) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF 34-PHE--VAL-41; LEU-89; PHE-91; ALA-122 AND 123-THR--VAL-125.
[5]	"Multi-PDZ domain protein MUPP1 is a cellular target for both adenovirus E4-ORF1 and high-risk papillomavirus type 18 E6 oncoproteins." Lee S.S., Glaunsinger B., Mantovani F., Banks L., Javier R.T. J. Virol. 74:9680-9693(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HUMAN MPDZ, MUTAGENESIS OF ALA-122; 123-THR--VAL-125 AND LEU-124.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S82508 Genomic DNA. Translation: AAB37504.1. AJ854486 Genomic DNA. Translation: CAI05991.1.
3D structure databases
ProteinModelPortal	P89079.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-431542.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008180. dUTP_pyroPase. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4OR1_ADE09

Accession

Primary (citable) accession number: P89079

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program