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P89079

- E4OR1_ADE09

UniProt

P89079 - E4OR1_ADE09

Protein

E4-ORF1

Gene

E4

Organism
Human adenovirus D serotype 9 (HAdV-9) (Human adenovirus 9)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Plays a key role in virus oncogenecity in animals. Binds and sequesters human MUPP1/MPDZ protein in the cytoplasm, preventing it from playing a role in cellular proliferation regulation. Induces cell transformation, probably by inactivating MPDZ protein.1 Publication

    Catalytic activityi

    dUTP + H2O = dUMP + diphosphate.

    GO - Molecular functioni

    1. dUTP diphosphatase activity Source: UniProtKB-EC
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. dUTP metabolic process Source: InterPro
    2. viral process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Host-virus interaction, Nucleotide metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E4-ORF1 (EC:3.6.1.23)
    Alternative name(s):
    Early E4 14.0 kDa protein
    ORF1
    Probable dUTPase E4 ORF1
    Gene namesi
    Name:E4
    OrganismiHuman adenovirus D serotype 9 (HAdV-9) (Human adenovirus 9)
    Taxonomic identifieri10527 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirusHuman mastadenovirus D
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    Host cytoplasm 1 Publication

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi34 – 418FHIPPHGV → IHIPPQGA: Complete loss of transformation.
    Mutagenesisi89 – 891L → Q: Complete loss of transformation. 1 Publication
    Mutagenesisi91 – 911F → S: Complete loss of transformation. 1 Publication
    Mutagenesisi122 – 1221A → D: Partial loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus. 2 Publications
    Mutagenesisi123 – 1253TLV → P: Complete loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus.
    Mutagenesisi124 – 1241L → P: Partial loss of transformation. Partial loss of binding to MPDZ. Localizes aberrantly in the nucleus. 1 Publication

    Keywords - Diseasei

    Oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 125125E4-ORF1PRO_0000221784Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Binds to human MPDZ.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Dlg1Q626964EBI-7401124,EBI-389325From a different organism.

    Protein-protein interaction databases

    IntActiP89079. 1 interaction.
    MINTiMINT-431542.

    Structurei

    3D structure databases

    ProteinModelPortaliP89079.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi122 – 1254PDZ-bindingSequence Analysis

    Sequence similaritiesi

    Belongs to the dUTPase family.Curated

    Family and domain databases

    Gene3Di2.70.40.10. 1 hit.
    InterProiIPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51283. SSF51283. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P89079-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAESLYAFID SPGGIAPVQE GTSNRYTFFC PESFHIPPHG VVLLHLKVSV    50
    LVPTGYQGRF MALNDYHARD ILTQSDVIFA GRRQELTVLL FNHTDRFLYV 100
    RKGHPVGTLL LERVIFPSVK IATLV 125
    Length:125
    Mass (Da):14,041
    Last modified:May 1, 1997 - v1
    Checksum:i75792A75E484BC09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S82508 Genomic DNA. Translation: AAB37504.1.
    AJ854486 Genomic DNA. Translation: CAI05991.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S82508 Genomic DNA. Translation: AAB37504.1 .
    AJ854486 Genomic DNA. Translation: CAI05991.1 .

    3D structure databases

    ProteinModelPortali P89079.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P89079. 1 interaction.
    MINTi MINT-431542.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.70.40.10. 1 hit.
    InterProi IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    [Graphical view ]
    Pfami PF00692. dUTPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51283. SSF51283. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mammary tumors induced by human adenovirus type 9: a role for the viral early region 4 gene."
      Javier R.T., Shenk T.
      Breast Cancer Res. Treat. 39:57-67(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Adenovirus type 9, complete sequence."
      Buettner W.H., Veres-Molnar S.K.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Isolate ATCC VR-1086 / Hicks / V-209-003-014.
    3. "Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats."
      Javier R.T.
      J. Virol. 68:3917-3924(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS ONCOGENE.
    4. "Mutant adenovirus type 9 E4 ORF1 genes define three protein regions required for transformation of CREF cells."
      Weiss R.S., Gold M.O., Vogel H., Javier R.T.
      J. Virol. 71:4385-4394(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF 34-PHE--VAL-41; LEU-89; PHE-91; ALA-122 AND 123-THR--VAL-125.
    5. "Multi-PDZ domain protein MUPP1 is a cellular target for both adenovirus E4-ORF1 and high-risk papillomavirus type 18 E6 oncoproteins."
      Lee S.S., Glaunsinger B., Mantovani F., Banks L., Javier R.T.
      J. Virol. 74:9680-9693(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN MPDZ, MUTAGENESIS OF ALA-122; 123-THR--VAL-125 AND LEU-124.

    Entry informationi

    Entry nameiE4OR1_ADE09
    AccessioniPrimary (citable) accession number: P89079
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3