SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P89079

- E4OR1_ADE09

UniProt

P89079 - E4OR1_ADE09

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
E4-ORF1
Gene
E4
Organism
Human adenovirus D serotype 9 (HAdV-9) (Human adenovirus 9)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a key role in virus oncogenecity in animals. Binds and sequesters human MUPP1/MPDZ protein in the cytoplasm, preventing it from playing a role in cellular proliferation regulation. Induces cell transformation, probably by inactivating MPDZ protein.1 Publication

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-EC
  2. protein binding Source: UniProtKB

GO - Biological processi

  1. dUTP metabolic process Source: InterPro
  2. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Host-virus interaction, Nucleotide metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
E4-ORF1 (EC:3.6.1.23)
Alternative name(s):
Early E4 14.0 kDa protein
ORF1
Probable dUTPase E4 ORF1
Gene namesi
Name:E4
OrganismiHuman adenovirus D serotype 9 (HAdV-9) (Human adenovirus 9)
Taxonomic identifieri10527 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirusHuman adenovirus D
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Host cytoplasm 1 Publication

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 418FHIPPHGV → IHIPPQGA: Complete loss of transformation. 1 Publication
Mutagenesisi89 – 891L → Q: Complete loss of transformation. 1 Publication
Mutagenesisi91 – 911F → S: Complete loss of transformation. 1 Publication
Mutagenesisi122 – 1221A → D: Partial loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus. 2 Publications
Mutagenesisi123 – 1253TLV → P: Complete loss of transformation. Complete loss of binding to MPDZ. Localizes aberrantly in the nucleus. 2 Publications
Mutagenesisi124 – 1241L → P: Partial loss of transformation. Partial loss of binding to MPDZ. Localizes aberrantly in the nucleus. 1 Publication

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 125125E4-ORF1
PRO_0000221784Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Binds to human MPDZ.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg1Q626964EBI-7401124,EBI-389325From a different organism.

Protein-protein interaction databases

IntActiP89079. 1 interaction.
MINTiMINT-431542.

Structurei

3D structure databases

ProteinModelPortaliP89079.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi122 – 1254PDZ-binding Reviewed prediction

Sequence similaritiesi

Belongs to the dUTPase family.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.

Sequencei

Sequence statusi: Complete.

P89079-1 [UniParc]FASTAAdd to Basket

« Hide

MAESLYAFID SPGGIAPVQE GTSNRYTFFC PESFHIPPHG VVLLHLKVSV    50
LVPTGYQGRF MALNDYHARD ILTQSDVIFA GRRQELTVLL FNHTDRFLYV 100
RKGHPVGTLL LERVIFPSVK IATLV 125
Length:125
Mass (Da):14,041
Last modified:May 1, 1997 - v1
Checksum:i75792A75E484BC09
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S82508 Genomic DNA. Translation: AAB37504.1.
AJ854486 Genomic DNA. Translation: CAI05991.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S82508 Genomic DNA. Translation: AAB37504.1 .
AJ854486 Genomic DNA. Translation: CAI05991.1 .

3D structure databases

ProteinModelPortali P89079.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P89079. 1 interaction.
MINTi MINT-431542.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mammary tumors induced by human adenovirus type 9: a role for the viral early region 4 gene."
    Javier R.T., Shenk T.
    Breast Cancer Res. Treat. 39:57-67(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Adenovirus type 9, complete sequence."
    Buettner W.H., Veres-Molnar S.K.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate ATCC VR-1086 / Hicks / V-209-003-014.
  3. "Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats."
    Javier R.T.
    J. Virol. 68:3917-3924(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS ONCOGENE.
  4. "Mutant adenovirus type 9 E4 ORF1 genes define three protein regions required for transformation of CREF cells."
    Weiss R.S., Gold M.O., Vogel H., Javier R.T.
    J. Virol. 71:4385-4394(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF 34-PHE--VAL-41; LEU-89; PHE-91; ALA-122 AND 123-THR--VAL-125.
  5. "Multi-PDZ domain protein MUPP1 is a cellular target for both adenovirus E4-ORF1 and high-risk papillomavirus type 18 E6 oncoproteins."
    Lee S.S., Glaunsinger B., Mantovani F., Banks L., Javier R.T.
    J. Virol. 74:9680-9693(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN MPDZ, MUTAGENESIS OF ALA-122; 123-THR--VAL-125 AND LEU-124.

Entry informationi

Entry nameiE4OR1_ADE09
AccessioniPrimary (citable) accession number: P89079
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi