Reviewed, UniProtKB/Swiss-Prot P87893 (POK11_HUMAN)

Names and origin

Protein names	Recommended name:     HERV-K_22q11.21 provirus ancestral Pol protein Alternative name(s):     HERV-K101 Pol protein Including the following 1 domains:     1- Recommended name:             Reverse transcriptase                 Short name=RT               EC=2.7.7.49
Organism	Homo sapiens (Human) [Complete proteome]
Taxonomic identifier	9606 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Domain	The LPQG and YXDD motifs are catalytically important and conserved among many retroviruses.
Miscellaneous	This protein is synthesized as Gag-Pro and Gag-Pro-Pol polyprotein precursors. These polyproteins are thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries. Exact N-terminus of this protein has not been formally described.
Sequence similarities	Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) subfamily. Contains 1 reverse transcriptase domain.
Caution	Truncated; premature stop codon after the reverse transcriptase domain.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase RNA-directed DNA polymerase Transferase
Technical term	Complete proteome ERV Transposable element
Gene Ontology (GO)
Biological process	RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	HERV-K_22q11.21 provirus ancestral Pol protein		PRO_0000186770
Regions
	Domain	57 – 245	189	Reverse transcriptase
	Motif	161 – 164	4	LPQG
	Motif	195 – 198	4	YXDD
Experimental info
	Sequence conflict	40	1	Q → P Ref.2
	Sequence conflict	53	1	A → V Ref.2
	Sequence conflict	95	1	V → L Ref.2
	Sequence conflict	111	1	M → I Ref.2
	Sequence conflict	117 – 254	138	Missing Ref.2
	Sequence conflict	222	1	S → N Ref.2
	Sequence conflict	222	1	S → N Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P87893-1 [UniParc]. Last modified September 13, 2004. Version 2. Checksum: 13185BF11BF970A0	FASTA	254	28,918
	10 20 30 40 50 60 NKSKKRRNRV SFLGAATIEP PKPIPLTWKT EKPVWVNQWQ LPKQKLEALH LLANEQLEKG 70 80 90 100 110 120 HIEPSFSPWN SPVFVIQKKS GKWRMLTDLR AVNAVIQPMG PLQPGLPSPA MIPKDWPLII 130 140 150 160 170 180 IDLKDCFFTI PLAEQDCEKF AFTIPAINNK EPATRFQWKV LPQGMLNSPT ICQTFVGRAL 190 200 210 220 230 240 QPVREKFSDC YIIHYIDDIL CAAETKDKLI DCYTFLQAEV ASAGLAIASD KIQTSTPFHY 250 LGMQIENRKI KPQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Many human endogenous retrovirus K (HERV-K) proviruses are unique to humans." Barbulescu M., Turner G., Seaman M.I., Deinard A.S., Kidd K.K., Lenz J. Curr. Biol. 9:861-868(1999) [PubMed: 10469592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed: 10591208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Characterization of human endogenous retrovirus type K (HERV-K) virus-like particles generated from recombinant baculoviruses." Toenjes R.R., Boller K., Limbach R., Lugert R., Kurth R. Virology 233:280-291(1997) [PubMed: 9217052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-222.

Web resources

Human Endogenous Retrovirus Database (HERVd)

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF164609 Genomic DNA. No translation available. AC007326 Genomic DNA. No translation available. Y10392 Genomic DNA. Translation: CAA71420.1.
IPI	IPI00456667.
3D structure databases
SMR	P87893. Positions 23-254.
ModBase	Search...
Proteomic databases
PRIDE	P87893.
Phylogenomic databases
HOVERGEN	P87893.
Enzyme and pathway databases
BRENDA	2.7.7.49. 247.
Gene expression databases
Genevestigator	P87893.
Family and domain databases
InterPro	IPR000477. Reverse_transcriptase. [Graphical view]
Pfam	PF00078. RVT_1. 1 hit. [Graphical view]
PROSITE	PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POK11_HUMAN

Accession

Primary (citable) accession number: P87893

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	September 13, 2004
Last modified:	January 19, 2010
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.