Reviewed,
UniProtKB/Swiss-Prot P87893 (POK11_HUMAN)
Last modified
January 19, 2010.
Version 56.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: HERV-K_22q11.21 provirus ancestral Pol protein Alternative name(s): HERV-K101 Pol protein Including the following 1 domains: 1- Recommended name: Reverse transcriptase Short name=RT EC=2.7.7.49 |
| Organism | Homo sapiens (Human) [Complete proteome] |
| Taxonomic identifier | 9606 [NCBI] |
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 254 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution. |
| Catalytic activity | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). |
| Domain | The LPQG and YXDD motifs are catalytically important and conserved among many retroviruses. |
| Miscellaneous | This protein is synthesized as Gag-Pro and Gag-Pro-Pol polyprotein precursors. These polyproteins are thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries. Exact N-terminus of this protein has not been formally described. |
| Sequence similarities | Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) subfamily. Contains 1 reverse transcriptase domain. |
| Caution | Truncated; premature stop codon after the reverse transcriptase domain. |
Ontologies
| Keywords | |
|---|---|
| Molecular function | Nucleotidyltransferase RNA-directed DNA polymerase Transferase |
| Technical term | Complete proteome ERV Transposable element |
| Gene Ontology (GO) | |
| Biological process | RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro |
| Molecular function | RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 254 | 254 | HERV-K_22q11.21 provirus ancestral Pol protein | PRO_0000186770 | |||||
Regions | |||||||||
| Domain | 57 – 245 | 189 | Reverse transcriptase | ||||||
| Motif | 161 – 164 | 4 | LPQG | ||||||
| Motif | 195 – 198 | 4 | YXDD | ||||||
Experimental info | |||||||||
| Sequence conflict | 40 | 1 | Q → P Ref.2 | ||||||
| Sequence conflict | 53 | 1 | A → V Ref.2 | ||||||
| Sequence conflict | 95 | 1 | V → L Ref.2 | ||||||
| Sequence conflict | 111 | 1 | M → I Ref.2 | ||||||
| Sequence conflict | 117 – 254 | 138 | Missing Ref.2 | ||||||
| Sequence conflict | 222 | 1 | S → N Ref.2 | ||||||
| Sequence conflict | 222 | 1 | S → N Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Many human endogenous retrovirus K (HERV-K) proviruses are unique to humans." Barbulescu M., Turner G., Seaman M.I., Deinard A.S., Kidd K.K., Lenz J. Curr. Biol. 9:861-868(1999) [PubMed: 10469592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. Wright H.Nature 402:489-495(1999) [PubMed: 10591208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "Characterization of human endogenous retrovirus type K (HERV-K) virus-like particles generated from recombinant baculoviruses." Toenjes R.R., Boller K., Limbach R., Lugert R., Kurth R. Virology 233:280-291(1997) [PubMed: 9217052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-222. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF164609 Genomic DNA. No translation available. AC007326 Genomic DNA. No translation available. Y10392 Genomic DNA. Translation: CAA71420.1. |
| IPI | IPI00456667. |
3D structure databases | |
| SMR | P87893. Positions 23-254. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | P87893. |
Phylogenomic databases | |
| HOVERGEN | P87893. |
Enzyme and pathway databases | |
| BRENDA | 2.7.7.49. 247. |
Gene expression databases | |
| Genevestigator | P87893. |
Family and domain databases | |
| InterPro | IPR000477. Reverse_transcriptase. [Graphical view] |
| Pfam | PF00078. RVT_1. 1 hit. [Graphical view] |
| PROSITE | PS50878. RT_POL. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | POK11_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P87893 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 22 Human chromosome 22: entries, gene names and cross-references to MIM |
| SIMILARITY comments Index of protein domains and families |

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