Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endogenous retrovirus group K member 10 Gag polyprotein

Gene

ERVK-10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The products of the Gag polyproteins of infectious retroviruses perform highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Endogenous Gag proteins may have kept, lost or modified their original function during evolution.

Miscellaneous

This Gag protein is encoded by a human specific provirus.
Intragenic, in the sixth intron of the SCGD gene.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri544 – 561CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri580 – 597CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endogenous retrovirus group K member 10 Gag polyprotein
Alternative name(s):
HERV-K10 Gag protein
HERV-K107 Gag protein
HERV-K_5q33.3 provirus ancestral Gag polyprotein
Short name:
Gag polyprotein
Gene namesi
Name:ERVK-10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:39004. ERVK-10.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi100616101.

Polymorphism and mutation databases

DMDMi134035263.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00001867532 – 666Endogenous retrovirus group K member 10 Gag polyproteinAdd BLAST665

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Post-translational modificationi

Myristoylation is essential for retroviral assembly. Alteration of the glycine residue leads to a block in the budding of particles and an accumulation of Gag inside the cell (By similarity).By similarity
Specific enzymatic cleavages may yield mature proteins.Curated

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PRIDEiP87889.

Structurei

3D structure databases

ProteinModelPortaliP87889.
SMRiP87889.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi168 – 257Pro-richAdd BLAST90
Compositional biasi611 – 666Gln-richAdd BLAST56

Domaini

HERV-K Gag polyprotein contains regions homologous to the matrix (MA), capsid (CA) and nucleocapsid (NC) proteins from infectious retroviruses. Evidence suggests that HERV-K(HML-2) Gag polyprotein can be cleaved into mature MA, CA and NC under certain circumstances. However, the exact boundaries as well as the size of processed Gag proteins have not been precisely determined yet.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri544 – 561CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri580 – 597CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG081547.
PhylomeDBiP87889.

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiView protein in InterPro
IPR003322. B_retro_matrix.
IPR000721. Gag_p24.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
IPR036875. Znf_CCHC_sf.
PfamiView protein in Pfam
PF02337. Gag_p10. 1 hit.
PF00607. Gag_p24. 1 hit.
PF00098. zf-CCHC. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004265. B_retro_matrix_N. 1 hit.
SMARTiView protein in SMART
SM00343. ZnF_C2HC. 2 hits.
SUPFAMiSSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiView protein in PROSITE
PS50158. ZF_CCHC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by ribosomal frameshifting. AlignAdd to basket

Note: This protein is synthesized as a Gag polypeptide and as a Gag-Pro-Pol polyprotein. The later is the precursor of the Pro and Pol proteins. It is thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries.
Isoform 1 (identifier: P87889-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQTKSKIKS KYASYLSFIK ILLKRGGVKV STKNLIKLFQ IIEQFCPWFP
60 70 80 90 100
EQGTSDLKDW KRIGKELKQA GRKGNIIPLT VWNDWAIIKA ALEPFQTEED
110 120 130 140 150
SISVSDAPGS CLIDCNENTR KKSQKETESL HCEYVAEPVM AQSTQNVDYN
160 170 180 190 200
QLQEVIYPET LKLEGKGPEL MGPSESKPRG TSPLPAGQVL VRLQPQKQVK
210 220 230 240 250
ENKTQPQVAY QYWPLAELQY RPPPESQYGY PGMPPAPQGR APYHQPPTRR
260 270 280 290 300
LNPMAPPSRQ GSELHEIIDK SRKEGDTEAW QFPVTLEPMP PGEGAQEGEP
310 320 330 340 350
PTVEARYKSF SIKMLKDMKE GVKQYGPNSP YMRTLLDSIA YGHRLIPYDW
360 370 380 390 400
EILAKSSLSP SQFLQFKTWW IDGVQEQVRR NRAANPPVNI DADQLLGIGQ
410 420 430 440 450
NWSTISQQAL MQNEAIEQVR AICLRAWEKI QDPGSTCPSF NTVRQGSKEP
460 470 480 490 500
YPDFVARLQD VAQKSIADEK AGKVIVELMA YENANPECQS AIKPLKGKVP
510 520 530 540 550
AGSDVISEYV KACDGIGGAM HKAMLMAQAI TGVVLGGQVR TFGGKCYNCG
560 570 580 590 600
QIGHLKKNCP VLNKQNITIQ ATTTGREPPD LCPRCKKGKH WASQCRSKFD
610 620 630 640 650
KNGQPLSGNE QRGQPQAPQQ TGAFPIQPFV PQGFQGQQPP LSQVFQGISQ
660
LPQYNNCPSP QAAVQQ
Length:666
Mass (Da):74,139
Last modified:March 6, 2007 - v4
Checksum:i7DC295738E81FDCA
GO

Sequence cautioni

P87889: The sequence AAA88030 differs from that shown. Reason: Frameshift at positions 213 and 231.Curated
P87889: The sequence AAA88031 differs from that shown. Reason: Frameshift at positions 213 and 231.Curated
P87889: The sequence AAD51796 differs from that shown. Reason: Frameshift at several positions. A -1 frameshift presumed to occur within the codons for the last amino acids in the Gag and Pro open reading frames.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55S → L in AC016577 (PubMed:15372022).Curated1
Sequence conflicti186A → V in CAA71416 (PubMed:9217052).Curated1
Sequence conflicti190L → P in AC016577 (PubMed:15372022).Curated1
Sequence conflicti207Q → P in AC016577 (PubMed:15372022).Curated1
Sequence conflicti215L → P in AC016577 (PubMed:15372022).Curated1
Sequence conflicti436T → A in CAA51306 (PubMed:8506289).Curated1
Sequence conflicti472G → R in CAA51306 (PubMed:8506289).Curated1
Sequence conflicti596R → C in CAA71416 (PubMed:9217052).Curated1
Sequence conflicti640P → L in AC016577 (PubMed:15372022).Curated1
Sequence conflicti659S → L in CAA51306 (PubMed:8506289).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14123 Genomic DNA. Translation: AAA88030.1. Sequence problems.
M14123 Genomic DNA. Translation: AAA88031.1. Sequence problems.
Y10390 Genomic DNA. Translation: CAA71416.1.
AF164613 Genomic DNA. Translation: AAD51796.1. Sequence problems.
X72791 mRNA. Translation: CAA51306.1.
AC016577 Genomic DNA. No translation available.
PIRiA24483. FOHUE1.
B24483. FOHUE2.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiGAK10_HUMAN
AccessioniPrimary (citable) accession number: P87889
Secondary accession number(s): P10263
, P10264, Q69385, Q9UKH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 6, 2007
Last modified: October 25, 2017
This is version 115 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, ERV, Reference proteome, Transposable element

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families