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Protein

Endogenous retrovirus group K member 10 Gag polyprotein

Gene

ERVK-10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The products of the Gag polyproteins of infectious retroviruses perform highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Endogenous Gag proteins may have kept, lost or modified their original function during evolution.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri544 – 56118CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri580 – 59718CCHC-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endogenous retrovirus group K member 10 Gag polyprotein
Alternative name(s):
HERV-K10 Gag protein
HERV-K107 Gag protein
HERV-K_5q33.3 provirus ancestral Gag polyprotein
Short name:
Gag polyprotein
Gene namesi
Name:ERVK-10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:39004. ERVK-10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Polymorphism and mutation databases

DMDMi134035263.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 666665Endogenous retrovirus group K member 10 Gag polyproteinPRO_0000186753Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis

Post-translational modificationi

Myristoylation is essential for retroviral assembly. Alteration of the glycine residue leads to a block in the budding of particles and an accumulation of Gag inside the cell (By similarity).By similarity
Specific enzymatic cleavages may yield mature proteins.Curated

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PRIDEiP87889.

Structurei

3D structure databases

ProteinModelPortaliP87889.
SMRiP87889. Positions 10-93, 307-434, 443-526, 541-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi168 – 25790Pro-richAdd
BLAST
Compositional biasi611 – 66656Gln-richAdd
BLAST

Domaini

HERV-K Gag polyprotein contains regions homologous to the matrix (MA), capsid (CA) and nucleocapsid (NC) proteins from infectious retroviruses. Evidence suggests that HERV-K(HML-2) Gag polyprotein can be cleaved into mature MA, CA and NC under certain circumstances. However, the exact boundaries as well as the size of processed Gag proteins have not been precisely determined yet.

Sequence similaritiesi

Contains 2 CCHC-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri544 – 56118CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri580 – 59718CCHC-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG081547.
PhylomeDBiP87889.

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR003322. B_retro_matrix.
IPR000721. Gag_p24.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF02337. Gag_p10. 1 hit.
PF00607. Gag_p24. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
ProDomiPD004265. B_retro_matrix_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by ribosomal frameshifting. AlignAdd to basket

Note: This protein is synthesized as a Gag polypeptide and as a Gag-Pro-Pol polyprotein. The later is the precursor of the Pro and Pol proteins. It is thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries.
Isoform 1 (identifier: P87889-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQTKSKIKS KYASYLSFIK ILLKRGGVKV STKNLIKLFQ IIEQFCPWFP
60 70 80 90 100
EQGTSDLKDW KRIGKELKQA GRKGNIIPLT VWNDWAIIKA ALEPFQTEED
110 120 130 140 150
SISVSDAPGS CLIDCNENTR KKSQKETESL HCEYVAEPVM AQSTQNVDYN
160 170 180 190 200
QLQEVIYPET LKLEGKGPEL MGPSESKPRG TSPLPAGQVL VRLQPQKQVK
210 220 230 240 250
ENKTQPQVAY QYWPLAELQY RPPPESQYGY PGMPPAPQGR APYHQPPTRR
260 270 280 290 300
LNPMAPPSRQ GSELHEIIDK SRKEGDTEAW QFPVTLEPMP PGEGAQEGEP
310 320 330 340 350
PTVEARYKSF SIKMLKDMKE GVKQYGPNSP YMRTLLDSIA YGHRLIPYDW
360 370 380 390 400
EILAKSSLSP SQFLQFKTWW IDGVQEQVRR NRAANPPVNI DADQLLGIGQ
410 420 430 440 450
NWSTISQQAL MQNEAIEQVR AICLRAWEKI QDPGSTCPSF NTVRQGSKEP
460 470 480 490 500
YPDFVARLQD VAQKSIADEK AGKVIVELMA YENANPECQS AIKPLKGKVP
510 520 530 540 550
AGSDVISEYV KACDGIGGAM HKAMLMAQAI TGVVLGGQVR TFGGKCYNCG
560 570 580 590 600
QIGHLKKNCP VLNKQNITIQ ATTTGREPPD LCPRCKKGKH WASQCRSKFD
610 620 630 640 650
KNGQPLSGNE QRGQPQAPQQ TGAFPIQPFV PQGFQGQQPP LSQVFQGISQ
660
LPQYNNCPSP QAAVQQ
Length:666
Mass (Da):74,139
Last modified:March 6, 2007 - v4
Checksum:i7DC295738E81FDCA
GO

Sequence cautioni

The sequence AAA88030 differs from that shown. Reason: Frameshift at positions 213 and 231. Curated
The sequence AAA88031 differs from that shown. Reason: Frameshift at positions 213 and 231. Curated
The sequence AAD51796 differs from that shown. Reason: Frameshift at several positions. A -1 frameshift presumed to occur within the codons for the last amino acids in the Gag and Pro open reading frames.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551S → L in AC016577 (PubMed:15372022).Curated
Sequence conflicti186 – 1861A → V in CAA71416 (PubMed:9217052).Curated
Sequence conflicti190 – 1901L → P in AC016577 (PubMed:15372022).Curated
Sequence conflicti207 – 2071Q → P in AC016577 (PubMed:15372022).Curated
Sequence conflicti215 – 2151L → P in AC016577 (PubMed:15372022).Curated
Sequence conflicti436 – 4361T → A in CAA51306 (PubMed:8506289).Curated
Sequence conflicti472 – 4721G → R in CAA51306 (PubMed:8506289).Curated
Sequence conflicti596 – 5961R → C in CAA71416 (PubMed:9217052).Curated
Sequence conflicti640 – 6401P → L in AC016577 (PubMed:15372022).Curated
Sequence conflicti659 – 6591S → L in CAA51306 (PubMed:8506289).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14123 Genomic DNA. Translation: AAA88030.1. Sequence problems.
M14123 Genomic DNA. Translation: AAA88031.1. Sequence problems.
Y10390 Genomic DNA. Translation: CAA71416.1.
AF164613 Genomic DNA. Translation: AAD51796.1. Sequence problems.
X72791 mRNA. Translation: CAA51306.1.
AC016577 Genomic DNA. No translation available.
PIRiA24483. FOHUE1.
B24483. FOHUE2.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14123 Genomic DNA. Translation: AAA88030.1. Sequence problems.
M14123 Genomic DNA. Translation: AAA88031.1. Sequence problems.
Y10390 Genomic DNA. Translation: CAA71416.1.
AF164613 Genomic DNA. Translation: AAD51796.1. Sequence problems.
X72791 mRNA. Translation: CAA51306.1.
AC016577 Genomic DNA. No translation available.
PIRiA24483. FOHUE1.
B24483. FOHUE2.

3D structure databases

ProteinModelPortaliP87889.
SMRiP87889. Positions 10-93, 307-434, 443-526, 541-606.
ModBaseiSearch...
MobiDBiSearch...

Polymorphism and mutation databases

DMDMi134035263.

Proteomic databases

PRIDEiP87889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GeneCardsiERVK-10.
HGNCiHGNC:39004. ERVK-10.
neXtProtiNX_P87889.
GenAtlasiSearch...

Phylogenomic databases

HOVERGENiHBG081547.
PhylomeDBiP87889.

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR003322. B_retro_matrix.
IPR000721. Gag_p24.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF02337. Gag_p10. 1 hit.
PF00607. Gag_p24. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
ProDomiPD004265. B_retro_matrix_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAK10_HUMAN
AccessioniPrimary (citable) accession number: P87889
Secondary accession number(s): P10263
, P10264, Q69385, Q9UKH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 6, 2007
Last modified: September 7, 2016
This is version 108 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This Gag protein is encoded by a human specific provirus.
Intragenic, in the sixth intron of the SCGD gene.

Keywords - Technical termi

Complete proteome, ERV, Reference proteome, Transposable element

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.