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Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (isolate RVA/Human/India/116E/1986/G9P8[11]) (RV-A)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plays a role in the inhibition of host innate immunity by inducing the degradation of key host factors required to activate interferon production such as IRF3, IRF5 or IRF7. Associates with components of cullin RING ligases (CRLs) including CUL1 or CUL3, which are essential multisubunit ubiquitination complexes, to modulate their activities. Recognizes the host NF-kappa-B regulator BTRC through the presence of a DSGXS motif in the C-terminal substrate recognition domain.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1UniRule annotation
Short name:
NSP1UniRule annotation
Alternative name(s):
NCVP2UniRule annotation
Non-structural RNA-binding protein 53UniRule annotation
Short name:
NS53UniRule annotation
OrganismiRotavirus A (isolate RVA/Human/India/116E/1986/G9P8[11]) (RV-A)
Taxonomic identifieri42567 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

  • host cytoskeleton UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003690741 – 486Non-structural protein 1Add BLAST486

Post-translational modificationi

The C-terminal region is phosphorylated by host CKII/CSNK2A1. Phosphorylation of the DSGXS motif is essential for host NF-kappa-B inhibition.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation. Interacts with host CUL1 and CUL3. Interacts with host BTRC.UniRule annotation

Structurei

3D structure databases

SMRiP87724.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 81RNA-bindingUniRule annotationAdd BLAST81
Regioni42 – 79Zinc-binding domainUniRule annotationAdd BLAST38
Regioni82 – 176Important for cytoskeleton localizationUniRule annotationAdd BLAST95
Regioni317 – 486Interaction with host IRF3UniRule annotationAdd BLAST170

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi479 – 483IKBKB-like degron (ILD) motifUniRule annotation5

Domaini

The integrity of the zinc-binding domain in NSP1 is important for degradation of host IRF3.UniRule annotation

Sequence similaritiesi

Belongs to the rotavirus NSP1 family.UniRule annotation

Family and domain databases

HAMAPiMF_04088. ROTA_NSP1. 1 hit.
InterProiView protein in InterPro
IPR002148. Rotavirus_NSP1.
PfamiView protein in Pfam
PF00981. Rota_NS53. 1 hit.

Sequencei

Sequence statusi: Complete.

P87724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATFKDACYH YKRINKLNQT VLKLGVNDTW RPSPPTKYKG WCLDCCQHTD
60 70 80 90 100
LTYCRGCTIY HVCQWCSQYG RCFLDDEPHL LRMRTFKNEV TKDNLKNLID
110 120 130 140 150
MYNTLFPITQ KIIHRFINNT RQHKCRNECM TQWYNHLLMP ITLQSLSIEL
160 170 180 190 200
DGDVYYIFGY YDSMNNINQT PFSFTNLVDI YDKLLLDDVN FVRMSFLPTS
210 220 230 240 250
LQREYALRYF SKSRFISEQR KCVNDSHFSI NVLENLYNPN FKVQITRNCS
260 270 280 290 300
ELSVDWNEAC KLVKNVSAYF DILKTSHVEF YSVSTRCRIF TRCKLEMASK
310 320 330 340 350
LIKPNYVTSN HKTLATEVRN CKWCSINNSY TVWNDFRIKK IYNNIFSFLR
360 370 380 390 400
ALVKSNVNIG HCSSQEKIYE YVENVLNVCD DKRWKTSIME IFNCLEPVEL
410 420 430 440 450
NDVKYVLFNY EINWDVINVL IHSIGKVPQI LTLENVITII QSIVYEWFDI
460 470 480
TYMRNTPMVT FTIDKLRRLH IGLKTVDSDS GISDVE
Length:486
Mass (Da):57,596
Last modified:May 1, 1997 - v1
Checksum:i39FFD284F291ADC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85999 mRNA. Translation: AAB46986.1.

Similar proteinsi

Entry informationi

Entry nameiNSP1_ROTHU
AccessioniPrimary (citable) accession number: P87724
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 1, 1997
Last modified: March 28, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program