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P87691

- HEMA_INCYB

UniProt

P87691 - HEMA_INCYB

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Protein

Hemagglutinin-esterase-fusion glycoprotein

Gene

HE

Organism
Influenza C virus (strain C/Yamagata/4/1988)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell By similarity.By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641NucleophileBy similarity
Active sitei359 – 3591Charge relay systemBy similarity
Active sitei362 – 3621Charge relay systemBy similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. fusion of virus membrane with host plasma membrane Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)
Short name:
HEF
Cleaved into the following 2 chains:
Gene namesi
Name:HE
OrganismiInfluenza C virus (strain C/Yamagata/4/1988)
Taxonomic identifieri127958 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus C
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 77By similarity
Chaini8 – 439432Hemagglutinin-esterase-fusion glycoprotein chain 1PRO_0000039172Add
BLAST
Chaini440 – 648209Hemagglutinin-esterase-fusion glycoprotein chain 2PRO_0000039173Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi13 ↔ 576Interchain (between HEF1 and HEF2 chains)By similarity
Glycosylationi19 – 191N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi54 – 541N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi113 ↔ 158By similarity
Disulfide bondi133 ↔ 181By similarity
Glycosylationi137 – 1371N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi203 ↔ 245By similarity
Disulfide bondi222 ↔ 309By similarity
Disulfide bondi230 ↔ 282By similarity
Disulfide bondi339 ↔ 345By similarity
Glycosylationi388 – 3881N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HEF1-HEF2.By similarity

Structurei

3D structure databases

ProteinModelPortaliP87691.
SMRiP87691. Positions 8-434, 443-604.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini8 – 623616ExtracellularSequence AnalysisAdd
BLAST
Topological domaini645 – 6484CytoplasmicSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei624 – 64421HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 3326Fusion domain-1By similarityAdd
BLAST
Regioni34 – 144111Esterase domain-1By similarityAdd
BLAST
Regioni144 – 303160N-acetyl-9-O-acetylneuraminic acid bindingBy similarityAdd
BLAST
Regioni304 – 35855Esterase domain-2By similarityAdd
BLAST
Regioni359 – 644286Fusion domain-2By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.20.10. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P87691-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGLTEAEKI KICLQKQVNS SFSLHNGFGG NLYATEEKRM FELVKPKAGA
60 70 80 90 100
SVLNQSTWIG FGDSRTDQSN SAFPRSADVS AKTADKFRSL SGGSLMLSMF
110 120 130 140 150
GPPGKVDYLY QGCGKHKVFY EGVNWSPHAA IDCYRKNWTD IKLNFQKSIY
160 170 180 190 200
ELASQSHCMS LVNALDKTIP LQVTKGVAKN CNNSFLKNPA LYTQEVKPLE
210 220 230 240 250
QICGEENLAF FTLPTQFGTY ECKLHLVASC YFIYDSKEVY NKRGCGNYFQ
260 270 280 290 300
VIYDSSGKVV GGLDNRVSPY TGNSGDTPTM QCDMLQLKPG RYSVRSSPRF
310 320 330 340 350
LLMPERSYCF DMKEKGPVTA VQSIWGKGRE SDTAVDQACS STPGCMLIQK
360 370 380 390 400
QKPYIGEADD HHGDQEMREL LSGLDYEARC ISQSGWVNET SPFTEEYLLP
410 420 430 440 450
PKFGRCPLAA KEESIPKIPD GLLIPTSGTD TTVTKPKSRI FGIDDLIIGL
460 470 480 490 500
LFVAIVEAGI GGYLLGSRKE SGGGVTKESA EKGFEKIGND IQILRSSTNI
510 520 530 540 550
AIEKLNDRIS HDEQAIRDLT LEIENARSEA LLGELGIIRA LLVGNISIGL
560 570 580 590 600
QESLWELASE ITNRAGDLAV EVSPGCWIID NNICDQSCQN FIFKFNETAP
610 620 630 640
VPTIPPLDTK IDLQSDPFYW GSSLGLAITA AISLAALVIS GIAICRTK
Length:648
Mass (Da):71,142
Last modified:May 1, 1997 - v1
Checksum:iA0AE7820D26F3263
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti276 – 2761D → N in HMV-II adapted variant.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D63503 Genomic RNA. Translation: BAA09781.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D63503 Genomic RNA. Translation: BAA09781.1 .

3D structure databases

ProteinModelPortali P87691.
SMRi P87691. Positions 8-434, 443-604.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.20.10. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Selection of antigenically distinct variants of influenza C viruses by the host cell."
    Umetsu Y., Sugawara K., Nishimura H., Hongo S., Matsuzaki M., Kitame F., Nakamura K.
    Virology 189:740-744(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_INCYB
AccessioniPrimary (citable) accession number: P87691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3