P87630 (DUT_CWPXG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	DUT
ORF Names:	G2L

Organism

Cowpox virus (strain GRI-90 / Grishak) (CPV)

Taxonomic identifier

265871 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Cowpox virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Homo sapiens (Human) [TaxID: 9606]
Loxodonta africana (African elephant) [TaxID: 9785]
Microtus agrestis (Short-tailed field vole) [TaxID: 29092]
Mus musculus (Mouse) [TaxID: 10090]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological_process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000182946

Sequences

	Sequence		Length	Mass (Da)
	P87630 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: D562BC4F4827F1FA	FASTA	147	16,255
	10 20 30 40 50 60 MFNMNINSPV RFVKETNRAK SPTRQSPGAA GYDLYSAYDY TIPPGERQLI KTDISMSMPK 70 80 90 100 110 120 FCYGRIAPRS GLSLKGIDIG GGVIDEDYRG NIGVILINNG KCTFNVNTGD RIAQLIYQRI 130 140 YYPELEEVQS LDSTDRGAQG FGSTGLR

References

[1]

"Structure-function and organization of cowpox virus strain GRI-90 complete genome." Shchelkunov S.N., Safronov P.F., Totmenin A.V., Miheev M.V., Ryazankina O.I., Petrov N.A., Gutorov V.V., Kotwal G.J., Sandakhchiev L.S. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94355 Genomic DNA. Translation: CAA64116.1.
3D structure databases
ProteinModelPortal	P87630.
SMR	P87630. Positions 7-131.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_CWPXG

Accession

Primary (citable) accession number: P87630

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	May 1, 1997
Last modified:	October 3, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program