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Protein

T-box protein VegT-A

Gene

vegt-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor required for both mesoderm and endoderm formation in the embryo; signaling determinants and concentration levels may determine which germ layer is formed. Acts together with beta-catenin to activate genes that are responsible for mesoderm induction including wnt-8, eomes t/bra, siamois, mix1 and sox17. Directly binds to promoter DNA. Patterns the mesoderm along the dorsoventral and posterior axis. Activates siamois gene transcription when alone or in combination with beta-catenin, but inhibits siamois transcription in combination with pou5f1.1/oct-25.6 Publications

Miscellaneous

Was named Antipodean by PubMed:9012537 because of its localization to the vegetal hemisphere of the egg.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi57 – 230T-boxPROSITE-ProRule annotationAdd BLAST174

GO - Molecular functioni

  • sequence-specific DNA binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  • mesoderm formation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
T-box protein VegT-A
Alternative name(s):
Brachyury and Tbx-related protein
Short name:
T-box protein Brat
T-box protein Antipodean
Gene namesi
Name:vegt-a
Synonyms:apod, brat, vegt
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252142. vegt.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001844621 – 455T-box protein VegT-AAdd BLAST455

Proteomic databases

MaxQBiP87377.

Expressioni

Tissue specificityi

Uniformly distributed in stage I oocytes but becomes localized to the vegetal hemisphere by stage II and remains so thereafter throughout oogenesis and the early embryonic cleavage stages. Zygotic expression parallels blastopore formation and shifts from dorsal expression in the marginal zone of late blastula and early gastrula stages to a ventral/lateral expression at the posterior end of later stage embryos. Expression is excluded from the notochord. In tailbud and tadpole stages, expressed exclusively in a subset of posterior Rohon-Beard neurons.4 Publications

Developmental stagei

Expressed both maternally and zygotically. Maternally expressed from early oogenesis. Zygotic expression occurs from late blastula and reaches maximum levels during gastrulation (stages 10.5-12). Levels decline at the time of blastopore closure (stage 13).3 Publications

Inductioni

By mesoderm inducing factors and TGF-beta family members including nodal/nr-1 acting in an autoregulatory loop, nodal2/nr-2 derriere, bFGF and activin B.4 Publications

Interactioni

Subunit structurei

Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and pouf5.1/oct-25. Interacts (via C-terminus) with tcf7l1/tcf3 (via N-terminus). Also interacts with the other POU-domain transcription factors pou5f1.2/oct-91 and pou5f1.3/oct-60.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

IntActiP87377. 4 interactors.
MINTiMINT-4979289.

Structurei

3D structure databases

SMRiP87377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOVERGENiHBG003537.
KOiK10180.

Family and domain databases

CDDicd00182. TBOX. 1 hit.
Gene3Di2.60.40.820. 1 hit.
InterProiView protein in InterPro
IPR008967. p53-like_TF_DNA-bd.
IPR036960. T-box_sf.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiView protein in Pfam
PF00907. T-box. 1 hit.
PRINTSiPR00937. TBOX.
SMARTiView protein in SMART
SM00425. TBOX. 1 hit.
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiView protein in PROSITE
PS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P87377-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRNCCRECGL SAGHLEPEAS SNCASDVKSS PDMDSVSSQD SLYLPNTVGA
60 70 80 90 100
SLEDQDLWSQ FHQEGTEMII TKSGRRMFPQ CKIRLFGLHP YAKYMLLVDF
110 120 130 140 150
VPLDNFRYKW NKNQWEAAGK AEPHPPCRTY VHPDSPAPGA HWMKDPICFQ
160 170 180 190 200
KLKLTNNTLD QQGHIILHSM HRYKPRFHVV QSDDMYNSPW GLVQVFSFPE
210 220 230 240 250
TEFTSVTAYQ NEKITKLKIN HNPFAKGFRE QERSHKRDDV LKILQQSPSK
260 270 280 290 300
RQKRKKWEDS PEADISDFPK AICVKEESIM DPAGVYQNWV SDHEANQGLT
310 320 330 340 350
PHSPESEGAN QEQQVPTSSS NFYNKSHYRR SSQHLSSPFE LGEPSSRRLT
360 370 380 390 400
PDIATVPDSD PDSLAVFHVI PTQNSAPERT CSMNFSMEAP MKQPLRGAMY
410 420 430 440 450
SPYGADQWLV PAQGQYRPVG YTAYPTDLST QGAVAHPHSA MSDWSQYSLF

PYSCW
Length:455
Mass (Da):51,771
Last modified:December 15, 2009 - v2
Checksum:iCF7FAC946A1847C0
GO
Isoform 2 (identifier: P87377-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: MRNCCRECGLSAGHLEPEASSNCAS → MHSLP

Show »
Length:435
Mass (Da):49,729
Checksum:i0819EA667075981D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244L → H in AAB49478 (PubMed:9165117).Curated1
Sequence conflicti350T → P in AAI60675 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0385401 – 25MRNCC…SNCAS → MHSLP in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59483 mRNA. Translation: AAB93301.1.
X99905 mRNA. Translation: CAA68179.1.
U89707 mRNA. Translation: AAB49478.1.
BC130162 mRNA. Translation: AAI30163.1.
BC160675 mRNA. Translation: AAI60675.1.
BC170433 mRNA. Translation: AAI70433.1.
BC170435 mRNA. Translation: AAI70435.1.
RefSeqiNP_001081665.1. NM_001088196.1.
XP_018088382.1. XM_018232893.1. [P87377-2]
UniGeneiXl.1775.

Genome annotation databases

GeneIDi397986.
KEGGixla:397986.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVEGTA_XENLA
AccessioniPrimary (citable) accession number: P87377
Secondary accession number(s): A2BDB3
, B1H1N4, B7ZS85, P79930, P87386
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 2009
Last modified: October 25, 2017
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program