Reviewed, UniProtKB/Swiss-Prot P87355 (NDKM_COLLI)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase, mitochondrial
      Short name=NDP kinase, mitochondrial
      Short name=NDK
    EC=2.7.4.6

Gene names

Name:

NME4

Organism

Columba livia (Domestic pigeon)

Taxonomic identifier

8932 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Columbiformes › Columbidae › Columba

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Feedback inhibition by ADP.
Subcellular location	Mitochondrion intermembrane space.
Tissue specificity	Highest levels in the liver and kidney with lower levels in the heart, brain and breast muscle.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion Ref.2
	Chain	25 – 181	157	Nucleoside diphosphate kinase, mitochondrial		PRO_0000019431
Sites
	Active site	146	1	Pros-phosphohistidine intermediate By similarity
	Binding site	40	1	ATP By similarity
	Binding site	88	1	ATP By similarity
	Binding site	116	1	ATP By similarity
	Binding site	122	1	ATP By similarity
	Binding site	133	1	ATP By similarity
	Binding site	143	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P87355-1 [UniParc]. Last modified May 30, 2000. Version 3. Checksum: B892F7C44C001D2C	FASTA	181	20,342
	10 20 30 40 50 60 MFRGGTHRLR GQPGLSLPHG PRCYGSAPPE LQEKTLVLVK PDAVQRRLVG NVIQRFERRG 70 80 90 100 110 120 FKLVAMKLLQ ADQGLLDKHY QQLRQKPFYP ALLAYMTSGP LVAMVWEGYN VVRSTRAMVG 130 140 150 160 170 180 DTDSAVAAAG TIRGDFSMHV SRNVVHASDS VETAQREIGF WFQRNELVAW ESGDRDYTWG P

References

[1]	Mehus J.G., Milavetz B.I., Ivey M.A., Lambeth D.O. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Liver.
[2]	"Characterization and cloning of a nucleoside-diphosphate kinase targeted to matrix of mitochondria in pigeon." Lambeth D.O., Mehus J.G., Ivey M.A., Milavetz B.I. J. Biol. Chem. 272:24604-24611(1997) [PubMed: 9305928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-181, PROTEIN SEQUENCE OF 25-44. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U89605 mRNA. Translation: AAC78439.1. AF018267 Genomic DNA. Translation: AAC78438.1.
3D structure databases
HSSP	HSSP built from PDB template 1EHW based on UniProtKB O00746.
SMR	P87355. Positions 32-170.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P87355.
Enzyme and pathway databases
BRENDA	2.7.4.6. 3625.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKM_COLLI

Accession

Primary (citable) accession number: P87355
Secondary accession number(s): O42206

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)