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Protein

DNA polymerase subunit delta-2

Gene

cdc1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for replication of the leading DNA strand and for completion of lagging strand synthesis. It is essential for cell cycle progression.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • DNA strand elongation involved in DNA replication Source: PomBase
  • DNA synthesis involved in UV-damage excision repair Source: PomBase
  • mitotic DNA replication lagging strand elongation Source: PomBase
  • mitotic DNA replication leading strand elongation Source: PomBase
  • mitotic nuclear division Source: UniProtKB-KW
  • nucleic acid phosphodiester bond hydrolysis Source: GOC
  • premeiotic DNA replication Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, DNA replication, Mitosis

Enzyme and pathway databases

ReactomeiR-SPO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-SPO-174411. Polymerase switching on the C-strand of the telomere.
R-SPO-174414. Processive synthesis on the C-strand of the telomere.
R-SPO-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-SPO-174437. Removal of the Flap Intermediate from the C-strand.
R-SPO-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-SPO-5656169. Termination of translesion DNA synthesis.
R-SPO-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-SPO-5696400. Dual Incision in GG-NER.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-69091. Polymerase switching.
R-SPO-69109. Leading Strand Synthesis.
R-SPO-69166. Removal of the Flap Intermediate.
R-SPO-69183. Processive synthesis on the lagging strand.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase subunit delta-2 (EC:2.7.7.7)
Gene namesi
Name:cdc1
ORF Names:SPAC27E2.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC27E2.05.
PomBaseiSPAC27E2.05. cdc1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • delta DNA polymerase complex Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi293 – 2931G → S: Loss of function and does not bind cdc27.
Mutagenesisi381 – 3811W → R: No loss of function and binds cdc27.
Mutagenesisi389 – 3891D → G: Loss of function and does not bind cdc27.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462DNA polymerase subunit delta-2PRO_0000096173Add
BLAST

Proteomic databases

MaxQBiP87324.

Interactioni

Subunit structurei

Heterodimer with subunits of 125 kDa and 50 kDa. Interacts with cdc27, however the function of this complex is not known.

Binary interactionsi

WithEntry#Exp.IntActNotes
cdc27P302613EBI-865227,EBI-866919
pol3P303162EBI-865227,EBI-865207

Protein-protein interaction databases

BioGridi278560. 18 interactions.
IntActiP87324. 3 interactions.
MINTiMINT-4693284.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000189057.
InParanoidiP87324.
KOiK02328.
OMAiWQNIAPT.
OrthoDBiEOG092C3F88.
PhylomeDBiP87324.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR024826. DNA_pol_delta/II_ssu.
[Graphical view]
PANTHERiPTHR10416. PTHR10416. 1 hit.
PfamiPF04042. DNA_pol_E_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P87324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEVRHSIPC EETKRLELSL SQQTYSQQYA SIYFARLTAL RPRITEAASK
60 70 80 90 100
KWPDKQRLER VLDVKSDEDC WVVATAYMTT ALKPNVMNDV TQLHTIVTTT
110 120 130 140 150
EESPYVSPED AASGDIEYAL EDDYGRIDCS GSFLYDAGVV TGVVLAVLGH
160 170 180 190 200
EDEQGRFVVV DVCFPGIFSH SIPMTTDESQ AQPEYIAAVS GLGLSNDGIE
210 220 230 240 250
GIQVHQLVDF LRGTLPHVSS FSPSSIKRLI ILGNCLAPSI EIADSASASV
260 270 280 290 300
PIGKKKVKRY GYDTSAYNPN PTFQLDNFLD QVCSSIDVTL MPGPYDYSST
310 320 330 340 350
ILPQQPLHPA LLTKSKVWLG SSLQTVTNPT WLSLGNHFVL ATSGQNINDL
360 370 380 390 400
RKYHPKKSSL QCMENTLLWN HITPTSPDTL WCYPFTDKDT FVMEEMPDLY
410 420 430 440 450
LCGNQPKFGC KTVINEGNRI QLVSVPEFRK TGVLVLINMH TLNVEIIQFR
460
PMSVKAETPI TS
Length:462
Mass (Da):51,326
Last modified:July 1, 1997 - v1
Checksum:i3FF35E22D996580B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12562 mRNA. Translation: CAA73149.1.
Y12561 Genomic DNA. Translation: CAA73148.1.
CU329670 Genomic DNA. Translation: CAB11679.1.
PIRiT38453.
RefSeqiNP_594406.1. NM_001019837.2.

Genome annotation databases

EnsemblFungiiSPAC27E2.05.1; SPAC27E2.05.1:pep; SPAC27E2.05.
GeneIDi2542083.
KEGGispo:SPAC27E2.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12562 mRNA. Translation: CAA73149.1.
Y12561 Genomic DNA. Translation: CAA73148.1.
CU329670 Genomic DNA. Translation: CAB11679.1.
PIRiT38453.
RefSeqiNP_594406.1. NM_001019837.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278560. 18 interactions.
IntActiP87324. 3 interactions.
MINTiMINT-4693284.

Proteomic databases

MaxQBiP87324.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC27E2.05.1; SPAC27E2.05.1:pep; SPAC27E2.05.
GeneIDi2542083.
KEGGispo:SPAC27E2.05.

Organism-specific databases

EuPathDBiFungiDB:SPAC27E2.05.
PomBaseiSPAC27E2.05. cdc1.

Phylogenomic databases

HOGENOMiHOG000189057.
InParanoidiP87324.
KOiK02328.
OMAiWQNIAPT.
OrthoDBiEOG092C3F88.
PhylomeDBiP87324.

Enzyme and pathway databases

ReactomeiR-SPO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-SPO-174411. Polymerase switching on the C-strand of the telomere.
R-SPO-174414. Processive synthesis on the C-strand of the telomere.
R-SPO-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-SPO-174437. Removal of the Flap Intermediate from the C-strand.
R-SPO-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-SPO-5656169. Termination of translesion DNA synthesis.
R-SPO-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-SPO-5696400. Dual Incision in GG-NER.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-69091. Polymerase switching.
R-SPO-69109. Leading Strand Synthesis.
R-SPO-69166. Removal of the Flap Intermediate.
R-SPO-69183. Processive synthesis on the lagging strand.

Miscellaneous databases

PROiP87324.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR024826. DNA_pol_delta/II_ssu.
[Graphical view]
PANTHERiPTHR10416. PTHR10416. 1 hit.
PfamiPF04042. DNA_pol_E_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPOD2_SCHPO
AccessioniPrimary (citable) accession number: P87324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 1, 1997
Last modified: September 7, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.