Reviewed, UniProtKB/Swiss-Prot P87253 (KPC1_NEUCR)

Names and origin

Protein names

Recommended name:
    Protein kinase C-like
    EC=2.7.11.13

Gene names

ORF Names:

NCU06544

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	1142 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. Contains 2 REM (Hr1) repeats.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1142	1142	Protein kinase C-like		PRO_0000055744
Regions
	Repeat	5 – 77	73	REM 1
	Repeat	161 – 236	76	REM 2
	Domain	817 – 1076	260	Protein kinase
	Domain	1077 – 1142	66	AGC-kinase C-terminal
	Zinc finger	457 – 505	49	Phorbol-ester/DAG-type 1
	Zinc finger	525 – 576	52	Phorbol-ester/DAG-type 2
	Nucleotide binding	823 – 831	9	ATP By similarity
	Compositional bias	670 – 679	10	Poly-Ala
	Compositional bias	714 – 717	4	Poly-Gln
	Compositional bias	746 – 754	9	Poly-Gln
Sites
	Active site	942	1	Proton acceptor By similarity
	Binding site	846	1	ATP By similarity
Experimental info
	Sequence conflict	914	1	F → L in CAA72731. Ref.1
	Sequence conflict	1055 – 1056	2	EP → DA in CAA72731. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P87253-1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: A7462CDFD1848DAD	FASTA	1,142	127,972
	10 20 30 40 50 60 MNDEDKVHDI SKKIEREKAL INAAQAMRQQ TNNEQVRSKL DTQMREGRRN LEFFEEKLRE 70 80 90 100 110 120 LQMRRLGHGV DNMSLGASPM SGSHRQSVDD FEGYGAPSPP PKEDVRGHSS HQSQGSGPLM 130 140 150 160 170 180 PASAPYPGGP PDSNVPRARP NYTRLDLIKF DTPHLGPRIQ LMLSQIQFKL NVEEQYLKGI 190 200 210 220 230 240 EKMVQLYQME GDKKSKLDAA AKRVESKQKI VLLKQALKRY EELHIDIDVD GPDDDSINLP 250 260 270 280 290 300 ALRKPLSGTL SIRILAVKDV DHAPLGRFAR SPETFIAVKA EDIVVARTKP SRNDKWEAEF 310 320 330 340 350 360 HTFPVDKTNE IEFTVYDKPA EHPVPIAMLW VRISDIVEEL RRKKIEAEMT SAGWVSADRV 370 380 390 400 410 420 GSRAPPPQFP MGAQSPQFAA PPTSPGSQEQ NTMIPPQAPP PSQVVSQPVD GWFNLEPYGQ 430 440 450 460 470 480 IHLSFNFIKG ARPQGMDRLG RKGAVRQRKE EVHEMYGHKF VQKQFYNIMR CALCGDFLKY 490 500 510 520 530 540 SAGMQCEDCK YTCHTKCYTS VVTKCISKSN AETDPDEEKI NHRIPHRFIP FSNLTANWCC 550 560 570 580 590 600 HCGYMLPIGS KKNSRKCSEC ALTAHAQCVH LVPDFCGMSM AVANQILEGM RTQKKTHKDK 610 620 630 640 650 660 ASSMSERTLR PGSKTSISSG SIAQASTYSG STAYTSIASP EATEAAKLMY SQTTPRPGGP 670 680 690 700 710 720 DRTSTSSTTA SAAAAAAMAP KHSSQPSQAG SIPDFGGSPG YGRPDSRDDE YSAQQQQGYG 730 740 750 760 770 780 SPQQRKYNPA DYANIDAYSS PQARPQQQQQ QQQQTPQQVS PMYQQNPQTP ISKPQPVAPS 790 800 810 820 830 840 YDNQVVPSAS GVPVPTKKPL PSATDPGTGM RIGLDHFNFL AVLGKGNFGK VMLAETKKSR 850 860 870 880 890 900 KLYAIKVLKK EFIIENDEVE SIRSEKRVFL IANRERHPFL TNLHACFQTE TRVYFVMEYI 910 920 930 940 950 960 SGGDLMLHIQ RGMFGTKRAQ FYAAEVCLAL KYFHENGVIY RDLKLDNILL TLDGHIKIAD 970 980 990 1000 1010 1020 YGLCKEDMWY GSTTSTFCGT PEFMAPEILL DKKYGRAVDW WAFGVLIYQM LLQQSPFRGE 1030 1040 1050 1060 1070 1080 DEDEIYDAIL ADEPLYPIHM PRDSVSILQK LLTREPDQRL GSGPTDAQEI MSQPFFRNIN 1090 1100 1110 1120 1130 1140 WDDIYHKRVQ PPFLPQIKSA TDTSNFDSEF TSVTPVLTPV QSVLSQAMQE EFRGFSYTAD FE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a protein kinase C homologue from Neurospora crassa." Arpaia G., Macino G. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y12002 Genomic DNA. Translation: CAA72731.1. AABX02000028 Genomic DNA. Translation: EAA33015.1.
RefSeq	XP_962251.1.
3D structure databases
SMR	P87253. Positions 162-222, 439-509, 510-579, 814-1140.
ModBase	Search...
Genome annotation databases
GeneID	3878409.
KEGG	ncr:NCU06544.
NMPDR	fig|5141.1.peg.4332.
Phylogenomic databases
eggNOG	fuNOG05723.
OrthoDB	EOG97H763.
PhylomeDB	P87253.
Enzyme and pathway databases
BRENDA	2.7.11.13. 266.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR000861. HR1-like_rho-bd. IPR011072. HR1_rho-bd. IPR011009. Kinase-like_dom. IPR015745. PKC. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF02185. HR1. 2 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00742. Hr1. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPC1_NEUCR

Accession

Primary (citable) accession number: P87253
Secondary accession number(s): Q7RVG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 7, 2005
Last modified:	January 19, 2010
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)