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Protein

Crossover junction endonuclease mus81

Gene

mus81

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with eme1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).9 Publications

Cofactori

Mg2+1 Publication

GO - Molecular functioni

GO - Biological processi

  • DNA catabolic process, endonucleolytic Source: PomBase
  • double-strand break repair Source: PomBase
  • double-strand break repair via homologous recombination Source: PomBase
  • intra-S DNA damage checkpoint Source: PomBase
  • meiotic joint molecule formation Source: PomBase
  • mitotic DNA replication checkpoint Source: PomBase
  • postreplication repair Source: PomBase
  • reciprocal meiotic recombination Source: PomBase
  • replication fork processing Source: PomBase
  • resolution of meiotic recombination intermediates Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Meiosis

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endonuclease mus81 (EC:3.1.22.-)
Gene namesi
Name:mus81
ORF Names:SPCC4G3.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC4G3.05c.
PomBaseiSPCC4G3.05c. mus81.

Subcellular locationi

GO - Cellular componenti

  • Holliday junction resolvase complex Source: PomBase
  • mitochondrion Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi275 – 2751T → A in allele mus81.T239A; abrogates phosphorylation in response to hydroxyurea and abolishes interaction with cds1. 1 Publication
Mutagenesisi395 – 3962DD → AA in allele mus81-DD; abrogates endonuclease activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Crossover junction endonuclease mus81PRO_0000198860Add
BLAST

Post-translational modificationi

Phosphorylated in a cds1-dependent manner in response to hydroxyurea (HU) treatment. This phosphorylation promotes dissociation of mus81 from chromatin which may prevent cleavage of intact stalled replication forks.2 Publications

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with eme1. Associates with chromatin. Interacts with the FHA domain of cds1, and this may require prior phosphorylation of Thr-275.4 Publications

Protein-protein interaction databases

BioGridi275578. 41 interactions.
IntActiP87231. 1 interaction.
MINTiMINT-4692881.

Structurei

3D structure databases

ProteinModelPortaliP87231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini331 – 42999ERCC4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni267 – 30337Interaction with cds1Add
BLAST

Sequence similaritiesi

Belongs to the XPF family.Curated
Contains 1 ERCC4 domain.Curated

Phylogenomic databases

HOGENOMiHOG000198700.
InParanoidiP87231.
KOiK08991.
OMAiGWIKEWL.
OrthoDBiEOG76MKJ6.
PhylomeDBiP87231.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR033309. Mus81.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PANTHERiPTHR13451:SF0. PTHR13451:SF0. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P87231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDCGNPLFLQ WIQEWMEEST RRFPKSYQTW RKAYDSMKSC PITFHRPSQA
60 70 80 90 100
LALKGIGPTI CAKLEKKWNA YCLENNIPIS THNEQNDSHV NANKSSSETS
110 120 130 140 150
SEKPRSVKKP TTRKRKVYVP SYRSGAYSIL CALYMLNKHE FATKPQIVTM
160 170 180 190 200
AQPYCDSSFG SATDRNMRYT AWSAMKTLIT KNLVYQTGHP SKYCLTDDGE
210 220 230 240 250
EVCIRLAKVD DSFQRKHTVS NFSVSKSDDH DSSLCQPPNF VTSINKAGSS
260 270 280 290 300
SDHGGELHVT YCPVDHNEVS DGVETDIDVD QVDSLTGIHD HHIINNEQLI
310 320 330 340 350
DLTEQEKKQP NESNLSNLKI ETVLFSNCTV FLLIDTREIR SPLDRNLIID
360 370 380 390 400
KLTNDFGVNC QVRSLELGDA LWVARDMESG QEVVLDFVVE RKRYDDLVAS
410 420 430 440 450
IKDGRFHEQK ARLKKSGIRS VTYILEESSY DESFTESIRT AVSNTQVDQL
460 470 480 490 500
FHVRHTRSLE HSVSLLAEMT KQINLFYEKR KTLAVIPDLS IEAKTYESLR
510 520 530 540 550
EQLLKIDPST PYHISYHAFS SVLSKSSTLT VGDIFIRMLM TIKGISASKA
560 570 580 590 600
IEIQKKYPTF MHLFEAYEKS SSSQERNLLL NKTCQGYGFQ TIGPALSAKV

ASVFFPES
Length:608
Mass (Da):69,044
Last modified:May 16, 2006 - v2
Checksum:i481EBE3846931732
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAJ77393.1.
PIRiT41371.
RefSeqiXP_001713161.1. XM_001713109.1.

Genome annotation databases

EnsemblFungiiSPCC4G3.05c.1; SPCC4G3.05c.1:pep; SPCC4G3.05c.
GeneIDi2539004.
KEGGispo:SPCC4G3.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAJ77393.1.
PIRiT41371.
RefSeqiXP_001713161.1. XM_001713109.1.

3D structure databases

ProteinModelPortaliP87231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275578. 41 interactions.
IntActiP87231. 1 interaction.
MINTiMINT-4692881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC4G3.05c.1; SPCC4G3.05c.1:pep; SPCC4G3.05c.
GeneIDi2539004.
KEGGispo:SPCC4G3.05c.

Organism-specific databases

EuPathDBiFungiDB:SPCC4G3.05c.
PomBaseiSPCC4G3.05c. mus81.

Phylogenomic databases

HOGENOMiHOG000198700.
InParanoidiP87231.
KOiK08991.
OMAiGWIKEWL.
OrthoDBiEOG76MKJ6.
PhylomeDBiP87231.

Miscellaneous databases

NextBioi20800179.
PROiP87231.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR033309. Mus81.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PANTHERiPTHR13451:SF0. PTHR13451:SF0. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1."
    Boddy M.N., Lopez-Girona A., Shanahan P., Interthal H., Heyer W.-D., Russell P.
    Mol. Cell. Biol. 20:8758-8766(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDS1, PHOSPHORYLATION.
  3. "Mus81-Eme1 are essential components of a Holliday junction resolvase."
    Boddy M.N., Gaillard P.-H.L., McDonald W.H., Shanahan P., Yates J.R. III, Russell P.
    Cell 107:537-548(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COFACTOR, INTERACTION WITH EME1, SUBCELLULAR LOCATION, MUTAGENESIS OF 395-ASP-ASP-396.
  4. "Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks."
    Doe C.L., Ahn J.S., Dixon J., Whitby M.C.
    J. Biol. Chem. 277:32753-32759(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EME1.
  5. "Fission yeast Mus81.Eme1 Holliday junction resolvase is required for meiotic crossing over but not for gene conversion."
    Smith G.R., Boddy M.N., Shanahan P., Russell P.
    Genetics 165:2289-2293(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Cleavage of model replication forks by fission yeast Mus81-Eme1 and budding yeast Mus81-Mms4."
    Whitby M.C., Osman F., Dixon J.
    J. Biol. Chem. 278:6928-6935(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The endogenous Mus81-Eme1 complex resolves Holliday junctions by a nick and counternick mechanism."
    Gaillard P.-H.L., Noguchi E., Shanahan P., Russell P.
    Mol. Cell 12:747-759(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CHARACTERIZATION OF MUTANT 395-ALA-ALA-396.
  8. "Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis."
    Osman F., Dixon J., Doe C.L., Whitby M.C.
    Mol. Cell 12:761-774(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51."
    Doe C.L., Osman F., Dixon J., Whitby M.C.
    Nucleic Acids Res. 32:5570-5581(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress."
    Kai M., Boddy M.N., Russell P., Wang T.S.-F.
    Genes Dev. 19:919-932(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDS1, ASSOCIATION WITH CHROMATIN, PHOSPHORYLATION, MUTAGENESIS OF THR-275, CHARACTERIZATION OF MUTANT 395-ALA-ALA-396.

Entry informationi

Entry nameiMUS81_SCHPO
AccessioniPrimary (citable) accession number: P87231
Secondary accession number(s): Q2EEM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 16, 2006
Last modified: May 11, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

S.pombe appears to be critically dependent on the mus81-eme1 endonuclease for the resolution of meiotic crossovers. This may be due to the absence of an alternate pathway for crossover resolution such as the MSH4-MSH5 pathway which exists in S.cerevisiae and other eukaryotes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.