Reviewed, UniProtKB/Swiss-Prot P87228 (SERA_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative D-3-phosphoglycerate dehydrogenase
      Short name=3-PGDH
    EC=1.1.1.95

Gene names

ORF Names:

SPCC364.07, SPCC4G3.01

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glyceric acid: step 1/3.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro phosphoglycerate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Putative D-3-phosphoglycerate dehydrogenase		PRO_0000076017
Sites
	Active site	284	1	By similarity
	Active site	313	1	By similarity
	Active site	344	1	Proton donor By similarity
Amino acid modifications
	Modified residue	87	1	Phosphoserine Ref.2
	Modified residue	258	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P87228-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 1E6EA04BF137C628	FASTA	466	50,883
	10 20 30 40 50 60 MDIKGGRRGN VEDSLNKLSL SPPDNNSSFL SNHFQVRKSY SQAPARTLKP FASEDIKILL 70 80 90 100 110 120 LENVNQSALS NLKDEGYQVE FLKTSMSEDD LVEKIKGVHA IGIRSKTRLT RRVLEAADSL 130 140 150 160 170 180 IVIGCFCIGT NQVDLDFAAE RGIAVFNSPY ANSRSVAELV IGYIISLARQ VGDRSLELHR 190 200 210 220 230 240 GEWNKVSSGC WEIRGKTLGI IGYGHIGSQL SVLAEAMGLH VVYYDILPIM PLGSAKQLSS 250 260 270 280 290 300 LPELLHRADF VSLHVPASPE TKNMISSKEF AAMKEGSYLI NASRGTVVDI PALVDASKSG 310 320 330 340 350 360 KIAGAAIDVY PSEPAGNGKD KFVDSLNSWT SELTHCKNII LTPHIGGSTE EAQYNIGIEV 370 380 390 400 410 420 SEALTRYINE GNSIGAVNFP EVSLRSLTEA DRNAARVLFV HRNVPGVLRQ VNELFIDHNI 430 440 450 460 KSQFSDSRGD IAYLVADISD CTPGSLEALH QKLESLPCKI NTRLLY

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-258, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAB09778.1.
PIR	T41375.
RefSeq	NP_587837.1.
3D structure databases
HSSP	HSSP built from PDB template 1PSD based on UniProtKB P08328.
ModBase	Search...
Genome annotation databases
GeneID	2539490.
KEGG	spo:SPCC364.07.
NMPDR	fig|4896.1.peg.175.
Organism-specific databases
GeneDB_Spombe	SPCC364.07.
Phylogenomic databases
OMA	P87228. GIRSKTK.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002412-MON.
BRENDA	1.1.1.95. 653.
Gene expression databases
ArrayExpress	P87228.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERA_SCHPO

Accession

Primary (citable) accession number: P87228
Secondary accession number(s): O59798

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)