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Protein

Alkaline protease 2

Gene

alp2

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alkaline protease that allows assimilation of proteinaceous substrates. Acts as a significant virulence factor in invasive aspergillosis. Required for regular sporulation.1 Publication

Catalytic activityi

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei182Charge relay systemBy similarity1
Active sitei214Charge relay systemBy similarity1
Active sitei380Charge relay systemBy similarity1

GO - Molecular functioni

  • IgE binding Source: UniProtKB
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • asexual sporulation resulting in formation of a cellular spore Source: AspGD
  • pathogenesis Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processSporulation, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline protease 2 (EC:3.4.21.63)
Short name:
ALP2
Alternative name(s):
Autophagic serine protease alp2
Allergen: Asp f 18
Gene namesi
Name:alp2
ORF Names:AFUA_5G09210
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu5g09210

Subcellular locationi

Pathology & Biotechi

Allergenic propertiesi

Acts as a major allergen in patients suffering from extrinsic bronchial asthma. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3114 Asp f 18.0101
70 Asp f 18

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000041230417 – 1361 PublicationAdd BLAST120
ChainiPRO_0000412305137 – 495Alkaline protease 2Add BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi284N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi447N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi460N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Zymogen

Interactioni

Protein-protein interaction databases

STRINGi5085.CADAFUBP00005557

Structurei

3D structure databases

ProteinModelPortaliP87184
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini166 – 432Peptidase S8Add BLAST267

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000199176
InParanoidiP87184
KOiK01336
OMAiTKHKGSV
OrthoDBiEOG092C4A4B

Family and domain databases

CDDicd04077 Peptidases_S8_PCSK9_Proteinase, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR034193 PCSK9_ProteinaseK-like
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P87184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGYLSLSIL PLLVAASPVV VDSIHNGAAP ILSSMNAKEV PDSYIVVFKK
60 70 80 90 100
HVNAESAAAH HSWVQDIHSA QNERVELRKR SLFGFGEEAY LGLKNTFDIA
110 120 130 140 150
GSLVGYSGHF HEDVIEQVRK HPDVEYIEKD SEVHTMEDPT VEKSAPWGLA
160 170 180 190 200
RISHRDSLSF GTFNKYLYAS EGGEGVDAYT IDTGINVDHV DFEGRAQWGK
210 220 230 240 250
TIPTDDEDAD GNGHGTHCSG TIAGRKYGVA KKANLYAVKV LRSSGSGTMS
260 270 280 290 300
DVVAGVEWAV KSHLKKVKDA KDGKIKGFKG SVANMSLGGG KSRTLEAAVN
310 320 330 340 350
AGVEAGLHFA VAAGNDNADA CNYSPAAAEN PITVGASTLQ DERAYFSNYG
360 370 380 390 400
KCTDIFAPGL NILSTWIGSK HAVNTISGTS MASPHIAGLL AYFVSLQPSK
410 420 430 440 450
DSAFAVDELT PKKLKKDIIA IATQGALTDI PSDTPNLLAW NGGGSSNYTD
460 470 480 490
IIASGGYKVN ASVKDRFEGL VHKAEKLLTE ELGAIYSEIH DAAVA
Length:495
Mass (Da):52,640
Last modified:July 1, 1997 - v1
Checksum:iA358FE8D6EE533D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13338 mRNA Translation: CAA73782.1
AJ243145 Genomic DNA Translation: CAB45520.1
AAHF01000003 Genomic DNA Translation: EAL91680.1
RefSeqiXP_753718.1, XM_748625.1

Genome annotation databases

EnsemblFungiiCADAFUAT00006386; CADAFUAP00006386; CADAFUAG00006386
GeneIDi3510885
KEGGiafm:AFUA_5G09210

Similar proteinsi

Entry informationi

Entry nameiALP2_ASPFU
AccessioniPrimary (citable) accession number: P87184
Secondary accession number(s): E9QST4, Q4WUP6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: July 1, 1997
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health