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Protein

DNA-(apurinic or apyrimidinic site) lyase 2

Gene

apn2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. Provides the majority of the AP-endonuclease (APE) activity. Repairs phleomycin D1-induced DNA damage. Plays a role in oxidative damage repair.2 Publications

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Probably binds two magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Magnesium 1By similarity1
Active sitei151By similarity1
Active sitei191Proton donor/acceptorBy similarity1
Metal bindingi191Magnesium 2By similarity1
Metal bindingi193Magnesium 2By similarity1
Sitei193Transition state stabilizerBy similarity1
Sitei269Important for catalytic activityBy similarity1
Metal bindingi294Magnesium 1By similarity1

GO - Molecular functioni

  • class I DNA-(apurinic or apyrimidinic site) endonuclease activity Source: UniProtKB-EC
  • DNA-(apurinic or apyrimidinic site) endonuclease activity Source: PomBase
  • DNA binding Source: InterPro
  • double-stranded DNA 3'-5' exodeoxyribonuclease activity Source: PomBase
  • endonuclease activity Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • phosphodiesterase I activity Source: PomBase

GO - Biological processi

  • base-excision repair Source: GO_Central
  • cellular response to reactive oxygen species Source: PomBase
  • positive regulation of DNA N-glycosylase activity Source: PomBase

Keywordsi

Molecular functionLyase
Biological processDNA damage, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-(apurinic or apyrimidinic site) lyase 2 (EC:4.2.99.18)
Alternative name(s):
AP endonuclease 2
Apurinic-apyrimidinic endonuclease 2
Gene namesi
Name:apn2
ORF Names:SPBC3D6.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC3D6.10
PomBaseiSPBC3D6.10 apn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi402F → A: No change in activity; when associated with A-403. 1 Publication1
Mutagenesisi403F → A: No change in activity; when associated with A-402. 1 Publication1
Mutagenesisi456P → A: No change in activity; when associated with A-457 and A-458. 1 Publication1
Mutagenesisi457L → A: No change in activity; when associated with A-456 and A-458. 1 Publication1
Mutagenesisi458C → A: No change in activity; when associated with A-456 and A-457. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002000191 – 523DNA-(apurinic or apyrimidinic site) lyase 2Add BLAST523

Proteomic databases

MaxQBiP87175
PaxDbiP87175
PRIDEiP87175

PTM databases

iPTMnetiP87175

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei295Interaction with DNA substrateBy similarity1

Protein-protein interaction databases

BioGridi277204, 25 interactors
STRINGi4896.SPBC3D6.10.1

Structurei

3D structure databases

ProteinModelPortaliP87175
SMRiP87175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA repair enzymes AP/ExoA family.Curated

Phylogenomic databases

InParanoidiP87175
KOiK10772
OMAiGAFTCWS
OrthoDBiEOG092C1JOF
PhylomeDBiP87175

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR004808 AP_endonuc_1
IPR020847 AP_endonuclease_F1_BS
IPR020848 AP_endonuclease_F1_CS
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PANTHERiPTHR22748 PTHR22748, 1 hit
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SUPFAMiSSF56219 SSF56219, 2 hits
TIGRFAMsiTIGR00633 xth, 1 hit
PROSITEiView protein in PROSITE
PS00726 AP_NUCLEASE_F1_1, 1 hit
PS00727 AP_NUCLEASE_F1_2, 1 hit
PS51435 AP_NUCLEASE_F1_4, 1 hit

Sequencei

Sequence statusi: Complete.

P87175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILSWNVNG IQNPFNYFPW NKKNSYKEIF QELQADVICV QELKMQKDSF
60 70 80 90 100
PQQYAVVEGF DSYFTFPKIR KGYSGVGFYV KKDVAIPVKA EEGITGILPV
110 120 130 140 150
RGQKYSYSEA PEHEKIGFFP KDIDRKTANW IDSEGRCILL DFQMFILIGV
160 170 180 190 200
YCPVNSGENR LEYRRAFYKA LRERIERLIK EGNRKIILVG DVNILCNPID
210 220 230 240 250
TADQKDIIRE SLIPSIMESR QWIRDLLLPS RLGLLLDIGR IQHPTRKGMF
260 270 280 290 300
TCWNTRLNTR PTNYGTRIDY TLATPDLLPW VQDADIMAEV MGSDHCPVYL
310 320 330 340 350
DLKEEYEGKK LSNFLSHSKE PPLLSTAHHS AYRPSKNIHS MFQHFNSMKK
360 370 380 390 400
NKNNSPTQSE NVSASASSGS SPTVSRANSV IDVDAYPPEK RRRKEQSKLL
410 420 430 440 450
SFFAKQKEEK EETNKTEDVS IEVLDNNNES DIGLTVKKKV ENGNAWKQIF
460 470 480 490 500
SERAPPLCEG HKEPCKYLTV RKPGINYGRK FWICARPVGE LIKNSNAVSE
510 520
EDTQPFQCRF FIWDSDWRAN SKD
Length:523
Mass (Da):60,313
Last modified:July 1, 1997 - v1
Checksum:iB2A79AE61579FA1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY483158 mRNA Translation: AAR83752.1
CU329671 Genomic DNA Translation: CAB09119.1
PIRiT40370
RefSeqiNP_595522.1, NM_001021431.2

Genome annotation databases

EnsemblFungiiSPBC3D6.10.1; SPBC3D6.10.1:pep; SPBC3D6.10
GeneIDi2540679
KEGGispo:SPBC3D6.10

Similar proteinsi

Entry informationi

Entry nameiAPN2_SCHPO
AccessioniPrimary (citable) accession number: P87175
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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