Reviewed, UniProtKB/Swiss-Prot P87172 (GPI3_SCHPO)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol N-acetylglucosaminyltransferase gpi3 subunit
    EC=2.4.1.198
Alternative name(s):
    GlcNAc-PI synthesis protein

Gene names

Name:	gpi3
ORF Names:	SPBC3D6.07

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subunit structure	Component of a Phosphatidylinositol N-acetylglucosaminyltransferase complex By similarity.
Sequence similarities	Belongs to the glycosyltransferase 1 family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phosphatidylinositol N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Phosphatidylinositol N-acetylglucosaminyltransferase gpi3 subunit		PRO_0000339138

Sequences

	Sequence		Length	Mass (Da)
	P87172-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 829C189D186A1919	FASTA	456	51,778
	10 20 30 40 50 60 MVSDFFFPQP GGIESHIFQL SQRLIDLGHK VIVITHAYKD RVGVRYLTNG LTVYYVPLHT 70 80 90 100 110 120 VYRETTFPSF FSFFPIFRNI VIRENIEIVH GHGSLSFLCH DAILHARTMG LKTCFTDHSL 130 140 150 160 170 180 FGFADAGSIV TNKLLKFTMS DVNHVICVSH TCRENTVLRA VLNPKRVSVI PNALVAENFQ 190 200 210 220 230 240 PDPSKASKDF LTIVVISRLY YNKGIDLLIA VIPRICAQHP KVRFVIAGDG PKSIDLEQMR 250 260 270 280 290 300 EKYMLQDRVE MLGSVRHDQV RDVMVRGHIY LHPSLTEAFG TVLVEAASCG LYVISTKVGG 310 320 330 340 350 360 VPEVLPSHMT RFARPEEDDL ADTLSSVITD YLDHKIKTET FHEEVKQMYS WIDVAERTEK 370 380 390 400 410 420 VYDSICSENN LRLIDRLKLY YGCGQWAGKL FCLLIAIDYL VMVLLEWIWP ASDIDPAVDR 430 440 450 VSSTFKISKQ NFDESLVLTD PKKKTKIKTA CLKDAQ

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB09127.1.
PIR	T40367.
RefSeq	NP_595519.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P87172.
Protein family/group databases
CAZy	GT4. Glycosyltransferase Family 4.
Genome annotation databases
GeneID	2540986.
GenomeReviews	Gene locus gpi3 in contig CU329671_GR.
KEGG	spo:SPBC3D6.07.
NMPDR	fig|4896.1.peg.1385.
Organism-specific databases
GeneDB_Spombe	SPBC3D6.07.
Phylogenomic databases
eggNOG	fuNOG06031.
HOGENOM	HBG315887.
OMA	HVICVSH.
OrthoDB	EOG9RR816.
PhylomeDB	P87172.
Gene expression databases
ArrayExpress	P87172.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. IPR013234. PIGA_GPI_anchor_biosynthesis. [Graphical view]
Pfam	PF00534. Glycos_transf_1. 1 hit. PF08288. PIGA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPI3_SCHPO

Accession

Primary (citable) accession number: P87172

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	July 1, 1997
Last modified:	January 19, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)