P87168 (RNZ2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease Z 2, mitochondrial
Short name=RNase Z 2
EC=3.1.26.11

Alternative name(s):
tRNA 3 endonuclease 2
tRNase Z 2

Gene names

Name:	trz2
ORF Names:	SPBC3D6.03c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. May be involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA. Ref.3
Catalytic activity	Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.
Cofactor	Zinc Probable.
Subcellular location	Mitochondrion. Cytoplasm Ref.2 Ref.3.
Sequence similarities	Belongs to the RNase Z family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial tRNA 3'-trailer cleavage, endonucleolytic Inferred from direct assay Ref.3. Source: PomBase
Cellular_component	cell division site Inferred from direct assay Ref.2. Source: PomBase cytosol Inferred from direct assay Ref.2. Source: PomBase mitochondrion Inferred from direct assay Ref.3. Source: PomBase
Molecular_function	3'-tRNA processing endoribonuclease activity Inferred from direct assay Ref.3. Source: PomBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 37	37	Mitochondrion Potential
	Chain	38 – 678	641	Ribonuclease Z 2, mitochondrial		PRO_0000315962

Sequences

	Sequence		Length	Mass (Da)
	P87168 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 6C10654707315B78	FASTA	678	75,987
	10 20 30 40 50 60 MKASLLVPRR ALLFGQLLPP KYSWYSVKRW QSQLTFRNKS KRNTNRIMLS VVSSLNPDSL 70 80 90 100 110 120 IAPLLCVSLD NRKYLIGSMG ELTQMKFRSQ ASNYGGKSVS VFLMPPSLQS LNAWGITAGL 130 140 150 160 170 180 FGYLQSSGIQ NTWGLHAPKP VISIIKKSHH LFSGSPLRLD LNSFSSEDNA DATNSFYLDE 190 200 210 220 230 240 PEFCTIKGNI YSNWSFLSFN SKEAAGVFNA DKALALGVPF GPSNGKLCAG EAVLSKDGTT 250 260 270 280 290 300 WIYPHQVVGP PRKRQYFYVL GCSSLSALNQ MSKHVDSFSD VYPTCIIHIL EKGIWGPEYI 310 320 330 340 350 360 KFLSHPKFSR AQHFISCIEL ASNNPVFQRN KGRNVLPACR DFAAFDIKPS TLDTQTQLPE 370 380 390 400 410 420 NTYVLKEETS MVLYDEQCKI SESPSYSPVK LAKKFSSFNP LPFENEGYTL DVLGTSATCP 430 440 450 460 470 480 TWRRSLSSYS VAIDGTVIML DCGEGAISQF FRQYGTNTEP MLRKLKAIFI THLHSDHYLG 490 500 510 520 530 540 LLNVLQAWNK ANTNNSMHIN IIGPKFLWQW LQRLKSPANL QALLNRIIFI IAKETVTTPL 550 560 570 580 590 600 QLTSDLSISS VPSIHINDSY SCIISHTKYG KLVYSGDTRP NEKLVKAGIG ASLLLHESTF 610 620 630 640 650 660 EDDLKHEAIQ RQHSTASEAL SVAQSMKAKA LILTHFSQRS YDADFLPPDW TIYPKSKTIY 670 ANDGLQWQQF QSKQRETI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"The fission yeast Schizosaccharomyces pombe has two distinct tRNase Z(L)s encoded by two different genes and differentially targeted to the nucleus and mitochondria." Gan X., Yang J., Li J., Yu H., Dai H., Liu J., Huang Y. Biochem. J. 435:103-111(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB09123.1.
PIR	T40362.
RefSeq	NP_595514.1. NM_001021423.2.
3D structure databases
HSSP	HSSP built from PDB template 1Y44 based on UniProtKB P54548.
ProteinModelPortal	P87168.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC3D6.03c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC3D6.03c.1; SPBC3D6.03c.1:pep; SPBC3D6.03c.
GeneID	2540985.
KEGG	spo:SPBC3D6.03c.
Organism-specific databases
PomBase	SPBC3D6.03c.
Phylogenomic databases
eggNOG	COG1234.
KO	K00784.
OrthoDB	EOG4H49DX.
Family and domain databases
InterPro	IPR001279. Beta-lactamas-like. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802100.

Entry information

Entry name

RNZ2_SCHPO

Accession

Primary (citable) accession number: P87168

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program