ID   EI2BE_CANAL             Reviewed;         732 AA.
AC   P87163; A0A1D8PEB7; Q59QB1;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 3.
DT   27-MAR-2024, entry version 109.
DE   RecName: Full=Translation initiation factor eIF2B subunit epsilon;
DE   AltName: Full=GCD complex subunit GCD6;
DE   AltName: Full=Guanine nucleotide exchange factor subunit GCD6;
DE   AltName: Full=eIF2B GDP-GTP exchange factor subunit epsilon;
GN   Name=GCD6; Synonyms=TIF225; OrderedLocusNames=CAALFM_C108600CA;
GN   ORFNames=CaO19.407, CaO19.8037;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 544-732.
RC   STRAIN=SFI-0124;
RX   PubMed=9161422; DOI=10.1016/s0378-1119(96)00844-x;
RA   Favre B., Ryder N.S.;
RT   "Cloning and expression of squalene epoxidase from the pathogenic yeast
RT   Candida albicans.";
RL   Gene 189:119-126(1997).
CC   -!- FUNCTION: Acts as a component of the translation initiation factor 2B
CC       (eIF2B) complex, which catalyzes the exchange of GDP for GTP on the
CC       eukaryotic initiation factor 2 (eIF2) complex gamma subunit. Its
CC       guanine nucleotide exchange factor activity is repressed when bound to
CC       eIF2 complex phosphorylated on the alpha subunit, thereby limiting the
CC       amount of methionyl-initiator methionine tRNA available to the ribosome
CC       and consequently global translation is repressed.
CC       {ECO:0000250|UniProtKB:P56287}.
CC   -!- SUBUNIT: Component of the translation initiation factor 2B (eIF2B)
CC       complex which is a heterodecamer of two sets of five different
CC       subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta
CC       and delta comprise a regulatory subcomplex and subunits epsilon and
CC       gamma comprise a catalytic subcomplex. Within the complex, the
CC       hexameric regulatory complex resides at the center, with the two
CC       heterodimeric catalytic subcomplexes bound on opposite sides.
CC       {ECO:0000250|UniProtKB:P56287}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:P56287}.
CC   -!- SIMILARITY: Belongs to the eIF-2B gamma/epsilon subunits family.
CC       {ECO:0000305}.
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DR   EMBL; CP017623; AOW26498.1; -; Genomic_DNA.
DR   EMBL; U69674; AAC49714.1; -; Genomic_DNA.
DR   RefSeq; XP_711895.2; XM_706802.2.
DR   AlphaFoldDB; P87163; -.
DR   SMR; P87163; -.
DR   BioGRID; 1229552; 2.
DR   STRING; 237561.P87163; -.
DR   EnsemblFungi; C1_08600C_A-T; C1_08600C_A-T-p1; C1_08600C_A.
DR   GeneID; 3646510; -.
DR   KEGG; cal:CAALFM_C108600CA; -.
DR   CGD; CAL0000183518; GCD6.
DR   VEuPathDB; FungiDB:C1_08600C_A; -.
DR   eggNOG; KOG1461; Eukaryota.
DR   HOGENOM; CLU_012507_1_0_1; -.
DR   InParanoid; P87163; -.
DR   OrthoDB; 5474157at2759; -.
DR   PRO; PR:P87163; -.
DR   Proteomes; UP000000559; Chromosome 1.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005851; C:eukaryotic translation initiation factor 2B complex; ISS:UniProtKB.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR   GO; GO:0003743; F:translation initiation factor activity; ISS:CGD.
DR   GO; GO:0031369; F:translation initiation factor binding; IBA:GO_Central.
DR   GO; GO:0002183; P:cytoplasmic translational initiation; ISS:UniProtKB.
DR   GO; GO:0006413; P:translational initiation; ISS:CGD.
DR   CDD; cd04197; eIF-2B_epsilon_N; 1.
DR   CDD; cd05787; LbH_eIF2B_epsilon; 1.
DR   CDD; cd11558; W2_eIF2B_epsilon; 1.
DR   Gene3D; 1.25.40.180; -; 1.
DR   Gene3D; 2.160.10.10; Hexapeptide repeat proteins; 1.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR035543; eIF-2B_epsilon_N.
DR   InterPro; IPR005835; NTP_transferase_dom.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   InterPro; IPR011004; Trimer_LpxA-like_sf.
DR   InterPro; IPR003307; W2_domain.
DR   InterPro; IPR044123; W2_eIF2B_epsilon.
DR   PANTHER; PTHR45887; TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON; 1.
DR   PANTHER; PTHR45887:SF1; TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON; 1.
DR   Pfam; PF00483; NTP_transferase; 1.
DR   Pfam; PF02020; W2; 1.
DR   SMART; SM00515; eIF5C; 1.
DR   SUPFAM; SSF48371; ARM repeat; 1.
DR   SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
DR   SUPFAM; SSF51161; Trimeric LpxA-like enzymes; 1.
DR   PROSITE; PS51363; W2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Initiation factor; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..732
FT                   /note="Translation initiation factor eIF2B subunit epsilon"
FT                   /id="PRO_0000156076"
FT   DOMAIN          559..726
FT                   /note="W2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00695"
SQ   SEQUENCE   732 AA;  81952 MW;  A7C2E826A30376BB CRC64;
     MAPKSKKQAA TQSKSKKSKD LVDERFQAIV LTDSFETRFM PLTAVHPRCL LPLANVPLIE
     YTLEFLANAG VNEVYLMCSA HADQIQEYIE NSKWMGDNSP FSVTTIMSIE SRSVGDTMRD
     LDNRGLIAGD FLLVSGDVVT NMDFSKALQF HKQKKAQDKD HIATMVLNQA SPLHRTRSQI
     DPAAFVLDKE TNRCIFYQSI PPVSGKKTCI SIDPELLEDF QGELQVRNDL IDCHVDICSP
     HVPQIFQENF DYQYLRSDFL KGVLTSDLLK KTIYAYISKD SSEYAARVES WSTYDAISQD
     ILARWCYPLV PDSNLVEGNS YSYELNNIYK EDKIILAQSC KIGTSTSIGR NSSVGEGTQI
     KNSVIGRNCT IGKNVVIENS YIWDNAVIKD NSVLNRSIVA ADAQIGNNVT LSPGSVIGFN
     VIIGDDKVIP HNVKIVETPI VTENEFGDFD DESNSEDENE YEDGNAVPVL AVKDVELVGE
     TGKGFAYESE IESGDEDDEE FVGNGTYSGI IYQMKSLNVS DDSIASVSNK KVKKHSHRRR
     LSMNSMISDN GGAFESDEGE EEEDFGVEGL ATVTRAIENN HDIDTALLEL NTLRMSMNVT
     YHDVRSVTTQ ALVNKIVDFI TTGTLTPQEA ATKIFTKWGI MFKRQVFSPE EEVDLLNIVE
     EKSSVLDKAY NQIVLFLGVK SFYDMEVVEE ENILKWWNDG ENDEVRTLAA KFITWLQEAD
     EEDSDEDDED SE
//