Reviewed, UniProtKB/Swiss-Prot P87131 (CYSK2_SCHPO)

Names and origin

Protein names

Recommended name:
    Cysteine synthase 2
      Short name=CSase 2
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase 2
    O-acetylserine (thiol)-lyase 2
      Short name=OAS-TL 2

Gene names

Name:	cys12
Synonyms:	cys1b
ORF Names:	SPAC3A12.17c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Ref.2 Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	cysteine synthase activity Ref.2 Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Cysteine synthase 2		PRO_0000167129
Regions
	Region	228 – 232	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	335	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	83	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P87131-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 94C3096B7761A64D	FASTA	395	43,086
	10 20 30 40 50 60 MAKKYQDLAS GIAMGAVFMY LLRRLYESLR VKSSADSLEE DIVNGVEGLI GNTKMVRIKS 70 80 90 100 110 120 LSQATGCDIL AKAEFLNPGN SPKDRVALQM IRTAEENGDL VPYQSNAVYE GTAGSTGISI 130 140 150 160 170 180 AMLCCSLGYD SRIYMPSDQS KEKSDILELL GAHVQRVTPA PIVDPNHFVN TARRNAANHT 190 200 210 220 230 240 VDESIPGKGY FANQFENPAN WQAHFNSTGP EIWRQCAGKL DAFIAGSGTG GTIAGISRYL 250 260 270 280 290 300 KSKDPSITVC LADPPGSGLY HKVLHGVMFD LAEREGTRRR HQVDTIVEGV GINRMTRNFS 310 320 330 340 350 360 IAEPLIDMAY RVTDEQAVAM SRYLVTHDGL FVGSSSAVNC VAAVRLAKKL GPGHRIVTLL 370 380 390 CDPGSRHFSK LYNEEFLRKK NIVPQVPSSL DFVEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Characterization of two genes encoding putative cysteine synthase required for cysteine biosynthesis in Schizosaccharomyces pombe." Fujita Y., Takegawa K. Biosci. Biotechnol. Biochem. 68:306-311(2004) [PubMed: 14981292] [Abstract] Cited for: IDENTIFICATION. Strain: ATCC 38399.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB08745.1.
PIR	T38685.
RefSeq	NP_593343.1.
3D structure databases
HSSP	HSSP built from PDB template 1J6N based on UniProtKB Q9WZD3.
ModBase	Search...
Protein-protein interaction databases
STRING	P87131.
Genome annotation databases
GeneID	2542583.
GenomeReviews	Gene locus cys12 in contig CU329670_GR.
KEGG	spo:SPAC3A12.17c.
NMPDR	fig|4896.1.peg.3313.
Organism-specific databases
GeneDB_Spombe	SPAC3A12.17c.
Phylogenomic databases
OMA	RVRPVSI.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001039-MON.
BRENDA	2.5.1.47. 653.
Gene expression databases
ArrayExpress	P87131.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK2_SCHPO

Accession

Primary (citable) accession number: P87131

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)