Reviewed, UniProtKB/Swiss-Prot P87118 (IPYR2_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative inorganic pyrophosphatase C3A12.02
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phosphohydrolase
      Short name=PPase

Gene names

ORF Names:

SPAC3A12.02

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Putative inorganic pyrophosphatase C3A12.02		PRO_0000137590
Sites
	Metal binding	122	1	Magnesium 1 By similarity
	Metal binding	127	1	Magnesium 1 By similarity
	Metal binding	127	1	Magnesium 2 By similarity
	Metal binding	159	1	Magnesium 1 By similarity
	Binding site	85	1	Inorganic pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P87118-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: FBE4A7EEF9132212	FASTA	286	32,899
	10 20 30 40 50 60 MASLAKNILQ FRSKITGKLN TPDFRVYCYK NNKPISFFHD VPLTSDKDTF NMVTEIPRWT 70 80 90 100 110 120 QAKCEISLTS PFHPIKQDLK NGKLRYVANS FPYHGFIWNY GALPQTWEDP NVIDSRTKMK 130 140 150 160 170 180 GDGDPLDVCE IGGSIGYIGQ IKQVKVLGAL GLIDQGETDW KILAIDINDP RAKLLNDISD 190 200 210 220 230 240 VQNLMPRLLP CTRDWFAIYK IPDGKPKNRF FFDGNYLPKS DALDIIAQCH QHWKVSRDRK 250 260 270 280 QYIKNFHNES VNNVDLINKI NSLKEEVSQN VSNYPSFPYF HTIPNL

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB08747.1.
PIR	T38670.
RefSeq	NP_593328.1.
3D structure databases
SMR	P87118. Positions 11-238.
ModBase	Search...
Protein-protein interaction databases
STRING	P87118.
Genome annotation databases
GeneID	2542957.
KEGG	spo:SPAC3A12.02.
NMPDR	fig|4896.1.peg.3298.
Organism-specific databases
GeneDB_Spombe	SPAC3A12.02.
Phylogenomic databases
eggNOG	fuNOG04419.
HOGENOM	HBG434750.
OMA	FNGQFKD.
PhylomeDB	P87118.
Enzyme and pathway databases
BRENDA	3.6.1.1. 653.
Gene expression databases
ArrayExpress	P87118.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR2_SCHPO

Accession

Primary (citable) accession number: P87118

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	January 19, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)