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Protein

RNA cytidine acetyltransferase

Gene

nat10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) at positions 1297 and 1815 in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis (PubMed:25402480). Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA-binding adapter protein tan1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation (By similarity).UniRule annotationBy similarity1 Publication

Catalytic activityi

A cytidine in 18S rRNA + ATP + acetyl-CoA + H2O = an N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA.UniRule annotation1 Publication
A cytidine in tRNA + ATP + acetyl-CoA + H2O = an N(4)-acetylcytidine in tRNAA + ADP + phosphate + CoA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei465ATPUniRule annotation1
Binding sitei727Acetyl-CoAUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi285 – 294ATPUniRule annotation10

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • rRNA cytidine N-acetyltransferase activity Source: PomBase

GO - Biological processi

  • negative regulation of G0 to G1 transition Source: PomBase
  • ribosomal small subunit biogenesis Source: PomBase
  • rRNA acetylation involved in maturation of SSU-rRNA Source: PomBase
  • tRNA acetylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA cytidine acetyltransferase1 PublicationUniRule annotation (EC:2.3.1.-UniRule annotation)
Alternative name(s):
18S rRNA cytosine acetyltransferaseUniRule annotation
Gene namesi
Name:nat101 PublicationUniRule annotation
ORF Names:SPAC20G8.09cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC20G8.09c.
PomBaseiSPAC20G8.09c. nat10.

Subcellular locationi

GO - Cellular componenti

  • 90S preribosome Source: PomBase
  • nucleolus Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi285G → D: Completely lacks 18S rRNA acetylation and produces only trace amounts of the 40S small ribosomal subunit. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002158891 – 1033RNA cytidine acetyltransferaseAdd BLAST1033

Proteomic databases

MaxQBiP87115.
PRIDEiP87115.

PTM databases

SwissPalmiP87115.

Interactioni

Subunit structurei

Interacts with tan1.UniRule annotation

Protein-protein interaction databases

BioGridi278978. 8 interactors.
MINTiMINT-4692370.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini560 – 694N-acetyltransferaseUniRule annotationAdd BLAST135

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni626 – 628Acetyl-CoA bindingUniRule annotation3
Regioni633 – 639Acetyl-CoA bindingUniRule annotation7

Sequence similaritiesi

Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.UniRule annotation
Contains 1 N-acetyltransferase domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000210833.
InParanoidiP87115.
KOiK14521.
OMAiHYKNTPD.
OrthoDBiEOG092C0F0X.
PhylomeDBiP87115.

Family and domain databases

HAMAPiMF_03211. RNA_acetyltr_Nat10. 1 hit.
InterProiIPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR033688. NAT10.
IPR032672. TmcA/NAT10/Kre33.
IPR013562. TmcA_N.
IPR027992. tRNA_bind_dom.
[Graphical view]
PANTHERiPTHR10925. PTHR10925. 1 hit.
PTHR10925:SF5. PTHR10925:SF5. 1 hit.
PfamiPF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
PF13725. tRNA_bind_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P87115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKKALDSRI PTLIKNGCQE KQRSFFVVVG DRARDQVVNL HWLLSQSKVA
60 70 80 90 100
ARPNVLWMYK KDLLGFTSHR KKRENKIKKE IKRGIRDPNS EDPFELFCSI
110 120 130 140 150
TNIRYCYYKE SEKILGQTYG MLVLQDFEAL TPNLLARTIE TVEGGGIVVL
160 170 180 190 200
LLHKLNSLKQ LYTMSMDIHS RYRTEAHSDV TARFNERFIL SLGNCENCLV
210 220 230 240 250
IDDELNVLPI SGGKNVKALP PTLEEDNSTQ NSIKELQESL GEDHPAGALV
260 270 280 290 300
GVTKTLDQAR AVLTFVESIV EKSLKGTVSL TAGRGRGKSA ALGLAIAAAI
310 320 330 340 350
AHGYSNIFIT SPSPENLKTL FEFIFKGFDA LNYEEHVDYD IIQSTNPAYH
360 370 380 390 400
NAIVRVNIFR DHRQTIQYIS PEDSNVLGQA ELVVIDEAAA IPLPLVRKLI
410 420 430 440 450
GPYLVFMAST INGYEGTGRS LSLKLLQQLR EQSRIYSGSG NNKSDSQSHI
460 470 480 490 500
SGRTLKEISL DEPIRYAMGD RIELWLNKLL CLDAASYVSR MATQGFPHPS
510 520 530 540 550
ECSLYRVSRD TLFSYHPISE AFLQRMMSLY VASHYKNSPN DLQLMSDAPA
560 570 580 590 600
HQLFVLLPPV DLKNPKLPDP ICVIQLALEG SISRESIMNS LSRGQRAGGD
610 620 630 640 650
LIPWLISQQF QDENFAALGG ARIVRIAVSP EHVKMGYGTR AMQLLHEYFE
660 670 680 690 700
GKFISASEEF KAVKHSLKRI GDEEIENTAL QTEKIHVRDA KTMPPLLLKL
710 720 730 740 750
SELQPEPLHY VGVSYGLTPS LQKFWKREGY CPLYLRQTAN DLTGEHTCVM
760 770 780 790 800
LRVLEGRDSE WLGAFAQNFY RRFLSLLGYQ FREFAAITAL SVLDACNNGT
810 820 830 840 850
KYVVNSTSKL TNEEINNVFE SYDLKRLESY SNNLLDYHVI VDLLPKLAHL
860 870 880 890 900
YFSGKFPDSV KLSPVQQSVL LALGLQYKTI DTLEKEFNLP SNQLLAMLVK
910 920 930 940 950
LSKKIMKCID EIETKDIEEE LGSNKKTESS NSKLPEFTPL QQSLEEELQE
960 970 980 990 1000
GADEAMLALR EKQRELINAI DLEKYAIRGN EEDWKAAENQ IQKTNGKGAR
1010 1020 1030
VVSIKGEKRK NNSLDASDKK TKEKPSSKKK FRK
Length:1,033
Mass (Da):116,463
Last modified:July 1, 1997 - v1
Checksum:i8432B313D818E135
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB08603.1.
PIRiT38131.
RefSeqiNP_593326.1. NM_001018757.2.

Genome annotation databases

EnsemblFungiiSPAC20G8.09c.1; SPAC20G8.09c.1:pep; SPAC20G8.09c.
GeneIDi2542520.
KEGGispo:SPAC20G8.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB08603.1.
PIRiT38131.
RefSeqiNP_593326.1. NM_001018757.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278978. 8 interactors.
MINTiMINT-4692370.

PTM databases

SwissPalmiP87115.

Proteomic databases

MaxQBiP87115.
PRIDEiP87115.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC20G8.09c.1; SPAC20G8.09c.1:pep; SPAC20G8.09c.
GeneIDi2542520.
KEGGispo:SPAC20G8.09c.

Organism-specific databases

EuPathDBiFungiDB:SPAC20G8.09c.
PomBaseiSPAC20G8.09c. nat10.

Phylogenomic databases

HOGENOMiHOG000210833.
InParanoidiP87115.
KOiK14521.
OMAiHYKNTPD.
OrthoDBiEOG092C0F0X.
PhylomeDBiP87115.

Miscellaneous databases

PROiP87115.

Family and domain databases

HAMAPiMF_03211. RNA_acetyltr_Nat10. 1 hit.
InterProiIPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR033688. NAT10.
IPR032672. TmcA/NAT10/Kre33.
IPR013562. TmcA_N.
IPR027992. tRNA_bind_dom.
[Graphical view]
PANTHERiPTHR10925. PTHR10925. 1 hit.
PTHR10925:SF5. PTHR10925:SF5. 1 hit.
PfamiPF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
PF13725. tRNA_bind_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAT10_SCHPO
AccessioniPrimary (citable) accession number: P87115
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.