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Protein

Mitochondrial import inner membrane translocase subunit TIM10

Gene

TIM10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. Compared to TIM9, it may function as a substrate sensor.13 Publications

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein transporter activity Source: SGD
  • unfolded protein binding Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31244-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM10
Alternative name(s):
Mitochondrial intermembrane protein MRS11
Gene namesi
Name:TIM10
Synonyms:MRS11
Ordered Locus Names:YHR005C-A
ORF Names:YHR005BC
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR005C-A.
SGDiS000003530. TIM10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40C → S: Induces impairment in folding and loss of zinc-binding. 2 Publications1
Mutagenesisi44C → S: Loss of function due to severely affected folding and the presence of non-native disulfide bonds; loss of zinc-binding. 2 Publications1
Mutagenesisi61C → S: Loss of function due to severely affected folding and the presence of non-native disulfide bonds; loss of zinc-binding. 2 Publications1
Mutagenesisi65C → S: Induces impairment in folding and loss of zinc-binding. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL1741194.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001936221 – 93Mitochondrial import inner membrane translocase subunit TIM10Add BLAST93

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 65
Disulfide bondi44 ↔ 61

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP87108.
PRIDEiP87108.

PTM databases

iPTMnetiP87108.

Interactioni

Subunit structurei

Heterohexamer; composed of 3 copies of TIM9 and 3 copies of TIM10, named soluble 70 kDa complex. Associates directly with the TIM12 component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM12P328302EBI-9115,EBI-11303
TIM18Q087492EBI-9115,EBI-30499
TIM9O747007EBI-9115,EBI-9108

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi36431. 25 interactors.
DIPiDIP-1141N.
IntActiP87108. 4 interactors.
MINTiMINT-4987878.

Chemistry databases

BindingDBiP87108.

Structurei

Secondary structure

193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni16 – 19Combined sources4
Helixi20 – 44Combined sources25
Helixi56 – 82Combined sources27

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DXRX-ray2.50B1-93[»]
ProteinModelPortaliP87108.
SMRiP87108.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP87108.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 31Interaction with transmembrane regions of transmembrane proteins in transitAdd BLAST31
Regioni73 – 93Required for heterohexamerizationAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 65Twin CX3C motifAdd BLAST26

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM10 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane.

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003068.
HOGENOMiHOG000211421.
InParanoidiP87108.
KOiK17778.
OMAiYREADLN.
OrthoDBiEOG092C5IPN.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR027100. Tim10.
IPR004217. Tim10/DDP_fam_Znf.
IPR027247. Tim10/Tim12.
[Graphical view]
PANTHERiPTHR11038. PTHR11038. 1 hit.
PTHR11038:SF16. PTHR11038:SF16. 1 hit.
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.

Sequencei

Sequence statusi: Complete.

P87108-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFLGFGGGQ PQLSSQQKIQ AAEAELDLVT DMFNKLVNNC YKKCINTSYS
60 70 80 90
EGELNKNESS CLDRCVAKYF ETNVQVGENM QKMGQSFNAA GKF
Length:93
Mass (Da):10,305
Last modified:July 1, 1997 - v1
Checksum:i70DD4764B944FF17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80875 Genomic DNA. Translation: CAB02581.1.
U10555 Genomic DNA. Translation: AAB68435.1.
BK006934 Genomic DNA. Translation: DAA06693.1.
PIRiS72314.
RefSeqiNP_011869.1. NM_001181427.1.

Genome annotation databases

EnsemblFungiiYHR005C-A; YHR005C-A; YHR005C-A.
GeneIDi856395.
KEGGisce:YHR005C-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80875 Genomic DNA. Translation: CAB02581.1.
U10555 Genomic DNA. Translation: AAB68435.1.
BK006934 Genomic DNA. Translation: DAA06693.1.
PIRiS72314.
RefSeqiNP_011869.1. NM_001181427.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DXRX-ray2.50B1-93[»]
ProteinModelPortaliP87108.
SMRiP87108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36431. 25 interactors.
DIPiDIP-1141N.
IntActiP87108. 4 interactors.
MINTiMINT-4987878.

Chemistry databases

BindingDBiP87108.
ChEMBLiCHEMBL1741194.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

PTM databases

iPTMnetiP87108.

Proteomic databases

MaxQBiP87108.
PRIDEiP87108.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR005C-A; YHR005C-A; YHR005C-A.
GeneIDi856395.
KEGGisce:YHR005C-A.

Organism-specific databases

EuPathDBiFungiDB:YHR005C-A.
SGDiS000003530. TIM10.

Phylogenomic databases

GeneTreeiENSGT00390000003068.
HOGENOMiHOG000211421.
InParanoidiP87108.
KOiK17778.
OMAiYREADLN.
OrthoDBiEOG092C5IPN.

Enzyme and pathway databases

BioCyciYEAST:G3O-31244-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTraceiP87108.
PROiP87108.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR027100. Tim10.
IPR004217. Tim10/DDP_fam_Znf.
IPR027247. Tim10/Tim12.
[Graphical view]
PANTHERiPTHR11038. PTHR11038. 1 hit.
PTHR11038:SF16. PTHR11038:SF16. 1 hit.
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTIM10_YEAST
AccessioniPrimary (citable) accession number: P87108
Secondary accession number(s): D3DKU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.