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Protein

Beta-glucosidase A

Gene

bglA

Organism
Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass. Catalyzes the last step releasing glucose from the inhibitory cellobiose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei280 – 2801By similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00696.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase A (EC:3.2.1.21)
Alternative name(s):
Beta-D-glucoside glucohydrolase A
Cellobiase A
Gentiobiase A
Gene namesi
Name:bglA
Synonyms:bgl1
ORF Names:AKAW_01481
OrganismiAspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
Taxonomic identifieri1033177 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006812: Unassembled WGS sequence

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 860841Beta-glucosidase APRO_0000394096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi442 – 4421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi542 – 5421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi564 – 5641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi658 – 6581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi690 – 6901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi712 – 7121N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiP87076.
OrthoDBiEOG7HMS8F.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR019800. Glyco_hydro_3_AS.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 2 hits.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P87076-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRFTLIEAVA LTAVSLASAD ELAYSPPYYP SPWANGQGDW AQAYQRAVDI
60 70 80 90 100
VSQMTLAEKV NLTTGTGWEL ELCVGQTGGV PRLGVPGMCL QDSPLGVRDS
110 120 130 140 150
DYNSAFPSGM NVAATWDKNL AYLRGKAMGQ EFSDKGADIQ LGPAAGPLGR
160 170 180 190 200
SPDGGRNWEG FSPDPALSGV LFAETIKGIQ DAGVVATAKH YIAYEQEHFR
210 220 230 240 250
QAPEAQGYGF NISESGSANL DDKTMHELYL WPFADAIRAG AGAVMCSYNQ
260 270 280 290 300
INNSYGCQNS YTLNKLLKAE LGFQGFVMSD WAAHHAGVSG ALAGLDMSMP
310 320 330 340 350
GDVDYDSGTS YWGTNLTVSV LNGTVPQWRV DDMAVRIMAA YYKVGRDRLW
360 370 380 390 400
TPPNFSSWTR DEYGYKYYYV SEGPYEKVNH YVNVQRNHSE LIRRIGADST
410 420 430 440 450
VLLKNDGALP LTGKERLVAL IGEDAGSNPY GANGCSDRGC DNGTLAMGWG
460 470 480 490 500
SGTANFPYLV TPEQAISNEV LKNKNGVFTA TDNWAIDQIE ALAKTASVSL
510 520 530 540 550
VFVNADSGEG YINVDGNLGD RRNLTLWRNG DNVIKAAASN CNNTIVIIHS
560 570 580 590 600
VGPVLVNEWY DNPNVTAILW GGLPGQESGN SLADVLYGRV NPGAKSPFTW
610 620 630 640 650
GKTREAYQDY LVTEPNNGNG APQEDFVEGV FIDYRGFDKR NETPIYEFGY
660 670 680 690 700
GLSYTTFNYS NLEVQVLSAP AYEPASGETE AAPTFGEVGN ASNYLYPDGL
710 720 730 740 750
QKITKFIYPW LNSTDLEASS GDASYGQDSS DYLPEGATDG SAQPILPAGG
760 770 780 790 800
GPGGNPRLYD ELIRVSVTIK NTGKVAGDEV PQLYVSLGGP NEPKIVLRQF
810 820 830 840 850
ERITLQPSEE TKWSTTLTRR DLANWNVEKQ DWEITSYPKM VFVGSSSRKL
860
PLRASLPTVH
Length:860
Mass (Da):93,349
Last modified:March 21, 2012 - v2
Checksum:iDA0FEF8C42CDBA37
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti522 – 5221R → K in BAA19913. (PubMed:10584016)Curated
Sequence conflicti850 – 8501L → P in BAA19913. (PubMed:10584016)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003470 Genomic DNA. Translation: BAA19913.1.
DF126449 Genomic DNA. Translation: GAA83366.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003470 Genomic DNA. Translation: BAA19913.1.
DF126449 Genomic DNA. Translation: GAA83366.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiP87076.
OrthoDBiEOG7HMS8F.

Enzyme and pathway databases

UniPathwayiUPA00696.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR019800. Glyco_hydro_3_AS.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 2 hits.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The bglA gene of Aspergillus kawachii encodes both extracellular and cell wall-bound beta-glucosidases."
    Iwashita K., Nagahara T., Kimura H., Takano M., Shimoi H., Ito K.
    Appl. Environ. Microbiol. 65:5546-5553(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 178-189; 405-414 AND 603-618, SUBCELLULAR LOCATION, FUNCTION.
    Strain: NBRC 4308.
  2. "Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu."
    Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M.
    Eukaryot. Cell 10:1586-1587(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 4308.

Entry informationi

Entry nameiBGLA_ASPKW
AccessioniPrimary (citable) accession number: P87076
Secondary accession number(s): G7X8K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: March 21, 2012
Last modified: January 7, 2015
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.